Estrogen and mechanical loading-related regulation of estrogen receptor-β and apoptosis in tendinopathy

Jeng Long Hsieh, I. Ming Jou, Chao Liang Wu, Po Ting Wu, Ai Li Shiau, Hao Earn Chong, Yu Ting Lo, Po Chuan Shen, Shih Yao Chen

Research output: Contribution to journalArticle

Abstract

Female-dominant tendinopathies are musculoskeletal disorders caused by repetitive hand posture and motion; they are considered overuse syndromes. Both external mechanical stress and changes in hormone levels might affect disease progression. We have previously reported that estrogen receptor-β (ER)-β expression was associated with the pathogenesis of de Quervain's disease. To study the underlying mechanisms, a cyclic stretching culture system was applied to tendon tissue from ovariectomized (OVX) rats. Furthermore, a collagenase I-induced rat tendinopathy model was established to examine the association of ER-β with disease progression. Our results showed that ER-β expression and the number of apoptotic cells were higher and associated with disease severity in rats with tendinopathy. Mechanical stress altered the morphology of primary tenocytes and collagen fiber alignment in tendons, and up-regulated the expression of matrix metalloproteinase-9, ER-β, and interleukin-1β, as well as induced apoptosis in tenocytes and tendon tissue from OVX rats. This is the first report on the effects of ER-β and mechanical stress in tendinopathy. We hope these findings contribute to new pharmacological therapies targeting ER-β signaling pathways to treat tendon-related diseases.

Original languageEnglish
Article numbere0204603
JournalPloS one
Volume13
Issue number10
DOIs
Publication statusPublished - 2018 Oct

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Tendinopathy
Estrogen Receptors
estrogens
Tendons
Estrogens
apoptosis
Mechanical Stress
Apoptosis
Rats
tendons
mechanical stress
Disease Progression
De Quervain Disease
disease course
microbial collagenase
Tissue
rats
Cumulative Trauma Disorders
Estrogen Receptor alpha
musculoskeletal diseases

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Hsieh, Jeng Long ; Jou, I. Ming ; Wu, Chao Liang ; Wu, Po Ting ; Shiau, Ai Li ; Chong, Hao Earn ; Lo, Yu Ting ; Shen, Po Chuan ; Chen, Shih Yao. / Estrogen and mechanical loading-related regulation of estrogen receptor-β and apoptosis in tendinopathy. In: PloS one. 2018 ; Vol. 13, No. 10.
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Estrogen and mechanical loading-related regulation of estrogen receptor-β and apoptosis in tendinopathy. / Hsieh, Jeng Long; Jou, I. Ming; Wu, Chao Liang; Wu, Po Ting; Shiau, Ai Li; Chong, Hao Earn; Lo, Yu Ting; Shen, Po Chuan; Chen, Shih Yao.

In: PloS one, Vol. 13, No. 10, e0204603, 10.2018.

Research output: Contribution to journalArticle

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AU - Shen, Po Chuan

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