Ethanol-induced alterations in the expression of endothelial-derived fibrinolytic components

C. D. Venkov; M. Su; Y. Shyr; D. E. Vaughan

doi:10.1016/S0268-9499(97)80104-X

Ethanol-induced alterations in the expression of endothelial-derived fibrinolytic components

C. D. Venkov, M. Su, Y. Shyr, D. E. Vaughan

Department of Statistics

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14 Citations (Scopus)

Abstract

Previous studies have identified a correlation between moderate alcohol consumption and plasma fibrinolytic activity. However, experimental studies designed to characterize the effect of alcohol on endothelial fibrinolytic parameters have provided inconsistent results. We investigated the effects of alcohol on the fibrinolytic components in a more simplified tissue culture system. The expression of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) in cultured human endothelial cells exposed to ethanol was studied using Northern blot hybridization. Treatment of endothelial cells with ethanol causes a gradual increase of the level of endothelial t-PA (mRNA) with a concomitant rapid decrease in PAI-1 mRNA levels. These divergent responses at the transcriptional level are associated with an increase in the extracellular t-PA activity in the conditioned medium of cells exposed to ethanol.

Original language	English
Pages (from-to)	115-118
Number of pages	4
Journal	Fibrinolysis and Proteolysis
Volume	11
Issue number	2
DOIs	https://doi.org/10.1016/S0268-9499(97)80104-X
Publication status	Published - 1997

All Science Journal Classification (ASJC) codes

Hematology

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10.1016/S0268-9499(97)80104-X

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title = "Ethanol-induced alterations in the expression of endothelial-derived fibrinolytic components",

abstract = "Previous studies have identified a correlation between moderate alcohol consumption and plasma fibrinolytic activity. However, experimental studies designed to characterize the effect of alcohol on endothelial fibrinolytic parameters have provided inconsistent results. We investigated the effects of alcohol on the fibrinolytic components in a more simplified tissue culture system. The expression of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) in cultured human endothelial cells exposed to ethanol was studied using Northern blot hybridization. Treatment of endothelial cells with ethanol causes a gradual increase of the level of endothelial t-PA (mRNA) with a concomitant rapid decrease in PAI-1 mRNA levels. These divergent responses at the transcriptional level are associated with an increase in the extracellular t-PA activity in the conditioned medium of cells exposed to ethanol.",

author = "Venkov, {C. D.} and M. Su and Y. Shyr and Vaughan, {D. E.}",

note = "Funding Information: This work was supported in part by a research grant from the Alcoholic Beverage Medical Research Foundation and by a Merit Award from the Department of Veteran Affairs Research Service. D.Vaughan is the recipient of a Clinical Investigator Award from the Department of Veteran Affairs. We gratefully acknowledge the expert technical assistance provided by Joseph Covington.",

year = "1997",

doi = "10.1016/S0268-9499(97)80104-X",

language = "English",

volume = "11",

pages = "115--118",

journal = "Fibrinolysis and Proteolysis",

issn = "1369-0191",

publisher = "Churchill Livingstone",

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T1 - Ethanol-induced alterations in the expression of endothelial-derived fibrinolytic components

AU - Venkov, C. D.

AU - Su, M.

AU - Shyr, Y.

AU - Vaughan, D. E.

N1 - Funding Information: This work was supported in part by a research grant from the Alcoholic Beverage Medical Research Foundation and by a Merit Award from the Department of Veteran Affairs Research Service. D.Vaughan is the recipient of a Clinical Investigator Award from the Department of Veteran Affairs. We gratefully acknowledge the expert technical assistance provided by Joseph Covington.

PY - 1997

Y1 - 1997

N2 - Previous studies have identified a correlation between moderate alcohol consumption and plasma fibrinolytic activity. However, experimental studies designed to characterize the effect of alcohol on endothelial fibrinolytic parameters have provided inconsistent results. We investigated the effects of alcohol on the fibrinolytic components in a more simplified tissue culture system. The expression of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) in cultured human endothelial cells exposed to ethanol was studied using Northern blot hybridization. Treatment of endothelial cells with ethanol causes a gradual increase of the level of endothelial t-PA (mRNA) with a concomitant rapid decrease in PAI-1 mRNA levels. These divergent responses at the transcriptional level are associated with an increase in the extracellular t-PA activity in the conditioned medium of cells exposed to ethanol.

AB - Previous studies have identified a correlation between moderate alcohol consumption and plasma fibrinolytic activity. However, experimental studies designed to characterize the effect of alcohol on endothelial fibrinolytic parameters have provided inconsistent results. We investigated the effects of alcohol on the fibrinolytic components in a more simplified tissue culture system. The expression of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) in cultured human endothelial cells exposed to ethanol was studied using Northern blot hybridization. Treatment of endothelial cells with ethanol causes a gradual increase of the level of endothelial t-PA (mRNA) with a concomitant rapid decrease in PAI-1 mRNA levels. These divergent responses at the transcriptional level are associated with an increase in the extracellular t-PA activity in the conditioned medium of cells exposed to ethanol.

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JO - Fibrinolysis and Proteolysis

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ER -

Ethanol-induced alterations in the expression of endothelial-derived fibrinolytic components

Abstract

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