TY - JOUR
T1 - Evaluating new bio-hydrogen producers
T2 - Clostridium perfringens strain JJC, Clostridium bifermentans strain WYM and Clostridium sp. strain Ade.TY
AU - Wong, Yee Meng
AU - Wu, Ta Yeong
AU - Ling, Tau Chuan
AU - Show, Pau Loke
AU - Lee, Sze Ying
AU - Chang, Jo Shu
AU - Ibrahim, Shaliza
AU - Juan, Joon Ching
N1 - Publisher Copyright:
© 2017 The Society for Biotechnology, Japan
PY - 2018/5
Y1 - 2018/5
N2 - Three newly discovered H2 producing bacteria namely Clostridium perfringens strain JJC, Clostridium bifermentans strain WYM and Clostridium sp. strain Ade.TY originated from landfill leachate sludge have demonstrated highly efficient H2 production. The maximum H2 production attained from these isolates are in the descending order of strain C. perfringens strain JJC > C. bifermentans strain WYM > Clostridium sp. strain Ade.TY with yield of 4.68 ± 0.12, 3.29 ± 0.11, and 2.87 ± 0.10 mol H2/mol glucose, respectively. The result has broken the conventional theoretical yield of 4 mol H2/mol glucose. These isolates were thermodynamically favourable with Gibbs free energy between −33 and −35 kJ/mol (under process conditions: pH 6, 37 °C and 5 g/L glucose). All three isolates favour butyrate pathway for H2 production with the ratio of acetate and butyrate of 0.77, 0.65 and 0.80 for strain JJC, WYM and Ade.TY, respectively. This study reported provides a new insight on the potential of unique bacteria in H2 production.
AB - Three newly discovered H2 producing bacteria namely Clostridium perfringens strain JJC, Clostridium bifermentans strain WYM and Clostridium sp. strain Ade.TY originated from landfill leachate sludge have demonstrated highly efficient H2 production. The maximum H2 production attained from these isolates are in the descending order of strain C. perfringens strain JJC > C. bifermentans strain WYM > Clostridium sp. strain Ade.TY with yield of 4.68 ± 0.12, 3.29 ± 0.11, and 2.87 ± 0.10 mol H2/mol glucose, respectively. The result has broken the conventional theoretical yield of 4 mol H2/mol glucose. These isolates were thermodynamically favourable with Gibbs free energy between −33 and −35 kJ/mol (under process conditions: pH 6, 37 °C and 5 g/L glucose). All three isolates favour butyrate pathway for H2 production with the ratio of acetate and butyrate of 0.77, 0.65 and 0.80 for strain JJC, WYM and Ade.TY, respectively. This study reported provides a new insight on the potential of unique bacteria in H2 production.
UR - http://www.scopus.com/inward/record.url?scp=85040522111&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85040522111&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2017.12.012
DO - 10.1016/j.jbiosc.2017.12.012
M3 - Article
C2 - 29352712
AN - SCOPUS:85040522111
SN - 1389-1723
VL - 125
SP - 590
EP - 598
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 5
ER -