Evaluation of cellular retinoic acid binding protein 2 gene expression through the retinoic acid pathway by co-incubation of Blastocystis ST-1 with HT29 cells in vitro

Chen Chieh Liao, Eing Ju Song, Tsuey Yu Chang, Wei Chen Lin, Hsiao-Sheng Liu, Lih Ren Chen, Lynn L.H. Huang, Jyh wei Shin

Research output: Contribution to journalArticle

Abstract

Blastocystis is a parasitic protist with a worldwide distribution that is commonly found in patients with colon and gastrointestinal pathological symptoms. Blastocystis infection has also commonly been reported in colorectal cancer and HIV/AIDS patients with gastrointestinal symptoms. To understand the pathway networks of gene regulation and the probable mechanisms influencing functions of HT-29 host cells in response to parasite infection, we examined the expression of 163 human oncogenes and kinases in human colon adenocarcinoma HT-29 cells co-incubated with Blastocystis by in-house cDNA microarray and PCR analysis. At least 10 genes were shown to be modified following Blastocystis co-incubation, including those with immunological, tumorigenesis, and antitumorigenesis functions. The expression of genes encoding cellular retinoic acid binding protein 2 (CRABP2) and proliferating cell nuclear antigen (PCNA) was markedly upregulated and downregulated, respectively. Reverse transcriptase-PCR validated the modified transcript expression of CRABP2 and other associated genes such as retinoic acid (RA)-related nuclear-receptor (RARα). Together, our data indicate that CRABP2, RARα, and PCNA expressions are involved in RA signaling regulatory networks that affect the growth, proliferation, and inflammation of HT-29 cells.

Original languageEnglish
Pages (from-to)1965-1975
Number of pages11
JournalParasitology Research
Volume115
Issue number5
DOIs
Publication statusPublished - 2016 May 1

Fingerprint

Blastocystis
HT29 Cells
Retinoic Acid Receptors
retinoic acid
Tretinoin
binding proteins
Proliferating Cell Nuclear Antigen
Cytoplasmic and Nuclear Receptors
Gene Expression
gene expression
Blastocystis Infections
Colon
proliferating cell nuclear antigen
Parasitic Diseases
Gene Regulatory Networks
signs and symptoms (animals and humans)
colon
cells
Microarray Analysis
Oligonucleotide Array Sequence Analysis

All Science Journal Classification (ASJC) codes

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Cite this

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title = "Evaluation of cellular retinoic acid binding protein 2 gene expression through the retinoic acid pathway by co-incubation of Blastocystis ST-1 with HT29 cells in vitro",
abstract = "Blastocystis is a parasitic protist with a worldwide distribution that is commonly found in patients with colon and gastrointestinal pathological symptoms. Blastocystis infection has also commonly been reported in colorectal cancer and HIV/AIDS patients with gastrointestinal symptoms. To understand the pathway networks of gene regulation and the probable mechanisms influencing functions of HT-29 host cells in response to parasite infection, we examined the expression of 163 human oncogenes and kinases in human colon adenocarcinoma HT-29 cells co-incubated with Blastocystis by in-house cDNA microarray and PCR analysis. At least 10 genes were shown to be modified following Blastocystis co-incubation, including those with immunological, tumorigenesis, and antitumorigenesis functions. The expression of genes encoding cellular retinoic acid binding protein 2 (CRABP2) and proliferating cell nuclear antigen (PCNA) was markedly upregulated and downregulated, respectively. Reverse transcriptase-PCR validated the modified transcript expression of CRABP2 and other associated genes such as retinoic acid (RA)-related nuclear-receptor (RARα). Together, our data indicate that CRABP2, RARα, and PCNA expressions are involved in RA signaling regulatory networks that affect the growth, proliferation, and inflammation of HT-29 cells.",
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Evaluation of cellular retinoic acid binding protein 2 gene expression through the retinoic acid pathway by co-incubation of Blastocystis ST-1 with HT29 cells in vitro. / Liao, Chen Chieh; Song, Eing Ju; Chang, Tsuey Yu; Lin, Wei Chen; Liu, Hsiao-Sheng; Chen, Lih Ren; Huang, Lynn L.H.; Shin, Jyh wei.

In: Parasitology Research, Vol. 115, No. 5, 01.05.2016, p. 1965-1975.

Research output: Contribution to journalArticle

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AU - Song, Eing Ju

AU - Chang, Tsuey Yu

AU - Lin, Wei Chen

AU - Liu, Hsiao-Sheng

AU - Chen, Lih Ren

AU - Huang, Lynn L.H.

AU - Shin, Jyh wei

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