Evaluation of the associations between the single nucleotide polymorphisms of the promoter region of the tumor necrosis factor-α gene and nasopharyngeal carcinoma

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Abstract

Background: Tumor necrosis factor-α (TNF-α) is a pro-inflammatory cytokine and may act as an endogenous tumor promoter. Single nucleotide polymorphisms (SNPs) of the TNF-α gene promoter region have been found to be associated with certain cancers. We conducted a case-control study to evaluate the association between these SNPs and nasopharyngeal carcinoma (NPC). Methods: We used polymerase chain reaction followed by restriction fragment length polymorphism analysis to determine the -308 TNF-α promoter genotypes of 89 NPC patients and 360 healthy controls. In 23 NPC patients and 50 controls, we determined the sequence from -1065 to -101 nucleotides of the TNF-α gene promoter region to detect SNPs. Results: In comparison with the controls, the NPC patients had higher proportions of men and carriage of IgA antibodies against the capsid antigen of Epstein-Barr virus, but had a similar carrier rate of the -308A allele (odds ratio [OR], 1.2; 95% confidence interval [CI], 0.7-2.0). The carriage of the -308A allele was not associated with the occurrence of NPC in comparison with -308G homozygosity. We also found no significant differences in the distributions of allelic variants of the -1031, -863, -857, and -806 loci of the TNF-α promoter region, but observed a lower carrier rate of the novel -806T allele in the NPC patients (OR, 0.3; 95% CI, 0.0-2.9). Conclusion: Allelic variants of the TNF-α promoter gene may not be used as biomarkers of susceptibility to NPC. The role of the -806T allele needs to be studied further.

Original languageEnglish
Pages (from-to)351-357
Number of pages7
JournalJournal of the Chinese Medical Association
Volume69
Issue number8
DOIs
Publication statusPublished - 2006 Jan 1

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Genetic Promoter Regions
Single Nucleotide Polymorphism
Tumor Necrosis Factor-alpha
Genes
Alleles
Odds Ratio
Confidence Intervals
Capsid
Nasopharyngeal carcinoma
Human Herpesvirus 4
Restriction Fragment Length Polymorphisms
Carcinogens
Immunoglobulin A
Case-Control Studies
Nucleotides
Biomarkers
Genotype
Cytokines
Antigens
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

@article{1947ec20aaff45a3a4f4beeb0d2459bd,
title = "Evaluation of the associations between the single nucleotide polymorphisms of the promoter region of the tumor necrosis factor-α gene and nasopharyngeal carcinoma",
abstract = "Background: Tumor necrosis factor-α (TNF-α) is a pro-inflammatory cytokine and may act as an endogenous tumor promoter. Single nucleotide polymorphisms (SNPs) of the TNF-α gene promoter region have been found to be associated with certain cancers. We conducted a case-control study to evaluate the association between these SNPs and nasopharyngeal carcinoma (NPC). Methods: We used polymerase chain reaction followed by restriction fragment length polymorphism analysis to determine the -308 TNF-α promoter genotypes of 89 NPC patients and 360 healthy controls. In 23 NPC patients and 50 controls, we determined the sequence from -1065 to -101 nucleotides of the TNF-α gene promoter region to detect SNPs. Results: In comparison with the controls, the NPC patients had higher proportions of men and carriage of IgA antibodies against the capsid antigen of Epstein-Barr virus, but had a similar carrier rate of the -308A allele (odds ratio [OR], 1.2; 95{\%} confidence interval [CI], 0.7-2.0). The carriage of the -308A allele was not associated with the occurrence of NPC in comparison with -308G homozygosity. We also found no significant differences in the distributions of allelic variants of the -1031, -863, -857, and -806 loci of the TNF-α promoter region, but observed a lower carrier rate of the novel -806T allele in the NPC patients (OR, 0.3; 95{\%} CI, 0.0-2.9). Conclusion: Allelic variants of the TNF-α promoter gene may not be used as biomarkers of susceptibility to NPC. The role of the -806T allele needs to be studied further.",
author = "Ho, {Sheng Yow} and Ying-Jan Wang and Huang, {Po Chang} and Sen-Tien Tsai and Chih-Hung Chen and Chen, {Helen H.W} and Chih-Jen Chang and How-Ran Guo",
year = "2006",
month = "1",
day = "1",
doi = "10.1016/S1726-4901(09)70272-2",
language = "English",
volume = "69",
pages = "351--357",
journal = "Journal of the Chinese Medical Association",
issn = "1726-4901",
publisher = "Elsevier Taiwan LLC",
number = "8",

}

TY - JOUR

T1 - Evaluation of the associations between the single nucleotide polymorphisms of the promoter region of the tumor necrosis factor-α gene and nasopharyngeal carcinoma

AU - Ho, Sheng Yow

AU - Wang, Ying-Jan

AU - Huang, Po Chang

AU - Tsai, Sen-Tien

AU - Chen, Chih-Hung

AU - Chen, Helen H.W

AU - Chang, Chih-Jen

AU - Guo, How-Ran

PY - 2006/1/1

Y1 - 2006/1/1

N2 - Background: Tumor necrosis factor-α (TNF-α) is a pro-inflammatory cytokine and may act as an endogenous tumor promoter. Single nucleotide polymorphisms (SNPs) of the TNF-α gene promoter region have been found to be associated with certain cancers. We conducted a case-control study to evaluate the association between these SNPs and nasopharyngeal carcinoma (NPC). Methods: We used polymerase chain reaction followed by restriction fragment length polymorphism analysis to determine the -308 TNF-α promoter genotypes of 89 NPC patients and 360 healthy controls. In 23 NPC patients and 50 controls, we determined the sequence from -1065 to -101 nucleotides of the TNF-α gene promoter region to detect SNPs. Results: In comparison with the controls, the NPC patients had higher proportions of men and carriage of IgA antibodies against the capsid antigen of Epstein-Barr virus, but had a similar carrier rate of the -308A allele (odds ratio [OR], 1.2; 95% confidence interval [CI], 0.7-2.0). The carriage of the -308A allele was not associated with the occurrence of NPC in comparison with -308G homozygosity. We also found no significant differences in the distributions of allelic variants of the -1031, -863, -857, and -806 loci of the TNF-α promoter region, but observed a lower carrier rate of the novel -806T allele in the NPC patients (OR, 0.3; 95% CI, 0.0-2.9). Conclusion: Allelic variants of the TNF-α promoter gene may not be used as biomarkers of susceptibility to NPC. The role of the -806T allele needs to be studied further.

AB - Background: Tumor necrosis factor-α (TNF-α) is a pro-inflammatory cytokine and may act as an endogenous tumor promoter. Single nucleotide polymorphisms (SNPs) of the TNF-α gene promoter region have been found to be associated with certain cancers. We conducted a case-control study to evaluate the association between these SNPs and nasopharyngeal carcinoma (NPC). Methods: We used polymerase chain reaction followed by restriction fragment length polymorphism analysis to determine the -308 TNF-α promoter genotypes of 89 NPC patients and 360 healthy controls. In 23 NPC patients and 50 controls, we determined the sequence from -1065 to -101 nucleotides of the TNF-α gene promoter region to detect SNPs. Results: In comparison with the controls, the NPC patients had higher proportions of men and carriage of IgA antibodies against the capsid antigen of Epstein-Barr virus, but had a similar carrier rate of the -308A allele (odds ratio [OR], 1.2; 95% confidence interval [CI], 0.7-2.0). The carriage of the -308A allele was not associated with the occurrence of NPC in comparison with -308G homozygosity. We also found no significant differences in the distributions of allelic variants of the -1031, -863, -857, and -806 loci of the TNF-α promoter region, but observed a lower carrier rate of the novel -806T allele in the NPC patients (OR, 0.3; 95% CI, 0.0-2.9). Conclusion: Allelic variants of the TNF-α promoter gene may not be used as biomarkers of susceptibility to NPC. The role of the -806T allele needs to be studied further.

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U2 - 10.1016/S1726-4901(09)70272-2

DO - 10.1016/S1726-4901(09)70272-2

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AN - SCOPUS:33748505667

VL - 69

SP - 351

EP - 357

JO - Journal of the Chinese Medical Association

JF - Journal of the Chinese Medical Association

SN - 1726-4901

IS - 8

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