Evaluation of the bruker biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of blood isolates of vibrio species

Wern Cherng Cheng, I. Shiow Jan, Jong Min Chen, Shih Hua Teng, Lee Jene Teng, Wang Huei Sheng, Wen Chien Ko, Po Ren Hsueh

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Among 56 blood isolates of Vibrio species identified by sequencing analysis of 16S rRNA and rpoB genes, the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) system correctly identified all isolates of Vibrio vulnificus (n = 20), V. parahaemolyticus (n = 2), and V. fluvialis (n = 1) but none of the isolates of serogroup non-O1/O139 (non-serogroup O1, non-O139) V. cholerae (n=33) to the species level. All of these serogroup non-O1/O139 V. cholerae isolates were correctly identified using the newly created MALDI-TOF MS database.

Original languageEnglish
Pages (from-to)1741-1744
Number of pages4
JournalJournal of Clinical Microbiology
Volume53
Issue number5
DOIs
Publication statusPublished - 2015 May 1

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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