Evidence of adenosine 5'-triphosphate release from nerve and P2x- purinoceptor mediated contraction during electrical stimulation of rat urinary bladder smooth muscle

Yat Ching Tong, Ying Cho Hung, Kazumasa Shinozuka, Masaru Kunitomo, Juei Tang Cheng

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Abstract

Purpose: We provided direct evidence for the existence of purinergic innervation in the rat urinary bladder. Materials and Methods: The non- adrenergic non-cholinergic (NANC) innervation was studied in 4-month-old Wistar rats. Electric-field stimulation (EFS) of the detrusor muscle strips in the presence of four autonomic blockers (atropine 10-6 M, guanethidine 10-6 M, phentolamine 10-6 M and propranolol 10-6 M) showed NANC contractions accounted for about 50% of the maximum contractile response. The adenyl purines released from nerves by EFS were detected by HPLC after conversion to ethenopurines. The amount of total purine released was frequency-dependent and could be totally suppressed by tetradotoxin (10- 6 M). The amount of ATP released was significantly greater than those for ADP, AMP and adenosine (p <0.05, n = 4). Desensitization induced by α, β- MeATP (10-6 to 10-4 M), a P2x receptor agonist, reduced the NANC contraction. In addition, the NANC contraction was also abolished by P2 receptor blocker suramin (10-4 to 10-3 M) and P2x receptor blocker PPADS (10-5 to 10-4 M.). Conclusion: The results of the present study give evidence to support purinergic nerve-mediated bladder smooth muscle contractions in the rat. Among the purine nucleotides, ATP is the dominant purinergic neurotransmitter released and P2x receptor activation is responsible for the NANC contractile response.

Original languageEnglish
Pages (from-to)1973-1977
Number of pages5
JournalJournal of Urology
Volume158
Issue number5
DOIs
Publication statusPublished - 1997 Nov

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Purinergic Receptors
Adrenergic Agents
Electric Stimulation
Smooth Muscle
Urinary Bladder
Adenosine Triphosphate
Purine Nucleotides
Guanethidine
Suramin
Purines
Phentolamine
Tetrodotoxin
Adenosine Monophosphate
Muscle Contraction
Atropine
Propranolol
Adenosine
Adenosine Diphosphate
Neurotransmitter Agents
Wistar Rats

All Science Journal Classification (ASJC) codes

  • Urology

Cite this

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title = "Evidence of adenosine 5'-triphosphate release from nerve and P2x- purinoceptor mediated contraction during electrical stimulation of rat urinary bladder smooth muscle",
abstract = "Purpose: We provided direct evidence for the existence of purinergic innervation in the rat urinary bladder. Materials and Methods: The non- adrenergic non-cholinergic (NANC) innervation was studied in 4-month-old Wistar rats. Electric-field stimulation (EFS) of the detrusor muscle strips in the presence of four autonomic blockers (atropine 10-6 M, guanethidine 10-6 M, phentolamine 10-6 M and propranolol 10-6 M) showed NANC contractions accounted for about 50{\%} of the maximum contractile response. The adenyl purines released from nerves by EFS were detected by HPLC after conversion to ethenopurines. The amount of total purine released was frequency-dependent and could be totally suppressed by tetradotoxin (10- 6 M). The amount of ATP released was significantly greater than those for ADP, AMP and adenosine (p <0.05, n = 4). Desensitization induced by α, β- MeATP (10-6 to 10-4 M), a P2x receptor agonist, reduced the NANC contraction. In addition, the NANC contraction was also abolished by P2 receptor blocker suramin (10-4 to 10-3 M) and P2x receptor blocker PPADS (10-5 to 10-4 M.). Conclusion: The results of the present study give evidence to support purinergic nerve-mediated bladder smooth muscle contractions in the rat. Among the purine nucleotides, ATP is the dominant purinergic neurotransmitter released and P2x receptor activation is responsible for the NANC contractile response.",
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Evidence of adenosine 5'-triphosphate release from nerve and P2x- purinoceptor mediated contraction during electrical stimulation of rat urinary bladder smooth muscle. / Tong, Yat Ching; Hung, Ying Cho; Shinozuka, Kazumasa; Kunitomo, Masaru; Cheng, Juei Tang.

In: Journal of Urology, Vol. 158, No. 5, 11.1997, p. 1973-1977.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evidence of adenosine 5'-triphosphate release from nerve and P2x- purinoceptor mediated contraction during electrical stimulation of rat urinary bladder smooth muscle

AU - Tong, Yat Ching

AU - Hung, Ying Cho

AU - Shinozuka, Kazumasa

AU - Kunitomo, Masaru

AU - Cheng, Juei Tang

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N2 - Purpose: We provided direct evidence for the existence of purinergic innervation in the rat urinary bladder. Materials and Methods: The non- adrenergic non-cholinergic (NANC) innervation was studied in 4-month-old Wistar rats. Electric-field stimulation (EFS) of the detrusor muscle strips in the presence of four autonomic blockers (atropine 10-6 M, guanethidine 10-6 M, phentolamine 10-6 M and propranolol 10-6 M) showed NANC contractions accounted for about 50% of the maximum contractile response. The adenyl purines released from nerves by EFS were detected by HPLC after conversion to ethenopurines. The amount of total purine released was frequency-dependent and could be totally suppressed by tetradotoxin (10- 6 M). The amount of ATP released was significantly greater than those for ADP, AMP and adenosine (p <0.05, n = 4). Desensitization induced by α, β- MeATP (10-6 to 10-4 M), a P2x receptor agonist, reduced the NANC contraction. In addition, the NANC contraction was also abolished by P2 receptor blocker suramin (10-4 to 10-3 M) and P2x receptor blocker PPADS (10-5 to 10-4 M.). Conclusion: The results of the present study give evidence to support purinergic nerve-mediated bladder smooth muscle contractions in the rat. Among the purine nucleotides, ATP is the dominant purinergic neurotransmitter released and P2x receptor activation is responsible for the NANC contractile response.

AB - Purpose: We provided direct evidence for the existence of purinergic innervation in the rat urinary bladder. Materials and Methods: The non- adrenergic non-cholinergic (NANC) innervation was studied in 4-month-old Wistar rats. Electric-field stimulation (EFS) of the detrusor muscle strips in the presence of four autonomic blockers (atropine 10-6 M, guanethidine 10-6 M, phentolamine 10-6 M and propranolol 10-6 M) showed NANC contractions accounted for about 50% of the maximum contractile response. The adenyl purines released from nerves by EFS were detected by HPLC after conversion to ethenopurines. The amount of total purine released was frequency-dependent and could be totally suppressed by tetradotoxin (10- 6 M). The amount of ATP released was significantly greater than those for ADP, AMP and adenosine (p <0.05, n = 4). Desensitization induced by α, β- MeATP (10-6 to 10-4 M), a P2x receptor agonist, reduced the NANC contraction. In addition, the NANC contraction was also abolished by P2 receptor blocker suramin (10-4 to 10-3 M) and P2x receptor blocker PPADS (10-5 to 10-4 M.). Conclusion: The results of the present study give evidence to support purinergic nerve-mediated bladder smooth muscle contractions in the rat. Among the purine nucleotides, ATP is the dominant purinergic neurotransmitter released and P2x receptor activation is responsible for the NANC contractile response.

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