TY - JOUR
T1 - Exopolysaccharides and antimicrobial biosurfactants produced by paenibacillus macerans TKU029
AU - Liang, Tzu Wen
AU - Wu, Chia Chen
AU - Cheng, Wei Ting
AU - Chen, Yu Chi
AU - Wang, Chuan Lu
AU - Wang, I. Li
AU - Wang, San Lang
N1 - Funding Information:
Acknowledgments This work was supported in part by a grant of the National Science Council, Taiwan (NSC 101-2621-M-032-005-and NSC 101-2811-M-032-015-).
PY - 2014/1
Y1 - 2014/1
N2 - Paenibacillus macerans TKU029 can produce exopolysaccharides (EPSs; 3.46 g/L) and a biosurfactant (1.78 g/L) in a medium with 2 % (w/v) squid pen powder as the sole carbon/nitrogen source. The biosurfactant can reduce the surface tension of water from 72.30 to 35.34 mN/m at a concentration of 2.76 g/L and reach an emulsification index of 56 % after a 24-h reaction with machine oil. This biosurfactant is stable at 121 C for 20 min, over a pH range from 3 to 11, and in <5 % salt solutions. It also shows significant antimicrobial activity, which remains active after treatment at 121 C and at pH values from 4 to 10, against Escherichia coli BCRC13086, Staphylococcus aureus BCRC10780, Fusarium oxysporum BCRC32121 and Aspergillus fumigatus BCRC30099. Furthermore, human skin shows from 37.3 to 44.3 % hydration after being treated with TKU029 EPSs for 180 min. These results imply that EPSs and the biosurfactant from this strain have potential in cosmetics, for removal of oil contamination, and as antimicrobial agents.
AB - Paenibacillus macerans TKU029 can produce exopolysaccharides (EPSs; 3.46 g/L) and a biosurfactant (1.78 g/L) in a medium with 2 % (w/v) squid pen powder as the sole carbon/nitrogen source. The biosurfactant can reduce the surface tension of water from 72.30 to 35.34 mN/m at a concentration of 2.76 g/L and reach an emulsification index of 56 % after a 24-h reaction with machine oil. This biosurfactant is stable at 121 C for 20 min, over a pH range from 3 to 11, and in <5 % salt solutions. It also shows significant antimicrobial activity, which remains active after treatment at 121 C and at pH values from 4 to 10, against Escherichia coli BCRC13086, Staphylococcus aureus BCRC10780, Fusarium oxysporum BCRC32121 and Aspergillus fumigatus BCRC30099. Furthermore, human skin shows from 37.3 to 44.3 % hydration after being treated with TKU029 EPSs for 180 min. These results imply that EPSs and the biosurfactant from this strain have potential in cosmetics, for removal of oil contamination, and as antimicrobial agents.
UR - http://www.scopus.com/inward/record.url?scp=84894481507&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84894481507&partnerID=8YFLogxK
U2 - 10.1007/s12010-013-0568-5
DO - 10.1007/s12010-013-0568-5
M3 - Article
C2 - 24122708
AN - SCOPUS:84894481507
SN - 0273-2289
VL - 172
SP - 933
EP - 950
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
IS - 2
ER -