Expanding the mesodermal transcriptional network by genome-wide identification of Zinc finger homeodomain 1 (Zfh1) targets

Wei Hang Wu, Tai Hong Kuo, Chia Wei Kao, Charles Girardot, Sheng Jou Hung, Tsung-lin Liu, Eileen E.M. Furlong, Ya-Hsin Liu

Research output: Contribution to journalArticle

Abstract

The Drosophila transcription factor (TF) Zfh1 has distinct roles compared to the cell lineage-determining TFs in almost all mesoderm-derived tissues. Here, we link Zfh1 to the well-characterized mesodermal transcriptional network. We identify five enhancers integrating upstream regulatory inputs from mesodermal TFs and directing zfh1 expression in mesoderm. Most downstream Zfh1-target genes are co-bound by mesodermal TFs, suggesting that Zfh1 and mesodermal TFs act on the same sets of co-regulated genes during the development of certain mesodermal tissues. Furthermore, we demonstrate that Zfh1 is critical for the expression of a hemocyte marker gene peroxidasin and helps restrict the activity of a hemocyte-specific enhancer of serpent to hemocyte-deriving head mesoderm, suggesting a potential role of Zfh1 in hemocyte development.

Original languageEnglish
Pages (from-to)1698-1710
Number of pages13
JournalFEBS Letters
Volume593
Issue number14
DOIs
Publication statusPublished - 2019 Jul 1

Fingerprint

Gene Regulatory Networks
Zinc Fingers
Zinc
Hemocytes
Genes
Genome
Mesoderm
Tissue
Cell Lineage
Drosophila
Transcription Factors
Head

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Wu, Wei Hang ; Kuo, Tai Hong ; Kao, Chia Wei ; Girardot, Charles ; Hung, Sheng Jou ; Liu, Tsung-lin ; Furlong, Eileen E.M. ; Liu, Ya-Hsin. / Expanding the mesodermal transcriptional network by genome-wide identification of Zinc finger homeodomain 1 (Zfh1) targets. In: FEBS Letters. 2019 ; Vol. 593, No. 14. pp. 1698-1710.
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Expanding the mesodermal transcriptional network by genome-wide identification of Zinc finger homeodomain 1 (Zfh1) targets. / Wu, Wei Hang; Kuo, Tai Hong; Kao, Chia Wei; Girardot, Charles; Hung, Sheng Jou; Liu, Tsung-lin; Furlong, Eileen E.M.; Liu, Ya-Hsin.

In: FEBS Letters, Vol. 593, No. 14, 01.07.2019, p. 1698-1710.

Research output: Contribution to journalArticle

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