Exploring physical stability characteristics of positively charged catanionic vesicle/DNA complexes

Chen Hsuan Lee, Yu Min Yang, Kuen Lin Leu, Hung Yin Lin, Chia Hua Liang, Chien-Hsiang Chang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cationic vesicles fabricated from various novel materials have been recently developed for DNA delivery applications, and the physical stability of the vesicle/DNA complexes played an important role in the transfection efficiency. In this study, positively charged catanionic vesicles composed of hexadecyltrimethylammonium-dodecylsulfate (HTMA-DS), ditetradecyldimethylammonium bromide (DTDAB), and cholesterol were prepared in buffer solution by a forced formation approach, and the association behavior of the catanionic vesicles with two types of DNA molecules with the consideration of the vesicle/DNA complex stability was explored by dynamic light scattering, fluorescence polarization, and DNA electrophoresis techniques. In a buffer solution of pH 7.4, HTMA-DS/DTDAB/cholesterol catanionic vesicles with high physical stability and positive charge character were successfully fabricated. A toxicity examination then implied that the catanionic vesicles have the potential of being applied in transfection applications. It is noted that the catanionic vesicle dispersions became unstable as prepared from a concentrated one by dilution due to the less charged vesicle surfaces and the more rigid vesicular bilayers, which could be explained by the enhanced dissolution of HTMA+ from the vesicles. The physical stability and charge character of the catanionic vesicle/DNA complexes were found strongly dependent on the mixing ratio of the vesicle to DNA. The formation of the catanionic vesicle/DNA complexes was also confirmed by an electrophoresis analysis. Moreover, not only the electrostatic repulsion between the vesicle/DNA complexes but also the molecular packing of the vesicular bilayers played roles in governing the physical stability of the vesicle/DNA complexes.

Original languageEnglish
Pages (from-to)2239-2247
Number of pages9
JournalColloid and Polymer Science
Volume293
Issue number8
DOIs
Publication statusPublished - 2015 Aug 24

Fingerprint

DNA
deoxyribonucleic acid
Cholesterol
cholesterol
electrophoresis
Electrophoresis
Bromides
bromides
Buffers
buffers
Dynamic light scattering
mixing ratios
Dispersions
toxicity
Dilution
Toxicity
dilution
Electrostatics
delivery
dissolving

All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry
  • Polymers and Plastics
  • Colloid and Surface Chemistry
  • Materials Chemistry

Cite this

Lee, Chen Hsuan ; Yang, Yu Min ; Leu, Kuen Lin ; Lin, Hung Yin ; Liang, Chia Hua ; Chang, Chien-Hsiang. / Exploring physical stability characteristics of positively charged catanionic vesicle/DNA complexes. In: Colloid and Polymer Science. 2015 ; Vol. 293, No. 8. pp. 2239-2247.
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Exploring physical stability characteristics of positively charged catanionic vesicle/DNA complexes. / Lee, Chen Hsuan; Yang, Yu Min; Leu, Kuen Lin; Lin, Hung Yin; Liang, Chia Hua; Chang, Chien-Hsiang.

In: Colloid and Polymer Science, Vol. 293, No. 8, 24.08.2015, p. 2239-2247.

Research output: Contribution to journalArticle

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