TY - JOUR
T1 - Exploring the high lipid production potential of a thermotolerant microalga using statistical optimization and semi-continuous cultivation
AU - Ho, Shih Hsin
AU - Chen, Ching Nen Nathan
AU - Lai, Yen Ying
AU - Lu, Wei Bin
AU - Chang, Jo Shu
N1 - Funding Information:
This work was supported in part by Grants NSC 102-2221-E-110-053-MY2 , NSC 101-3113-P-110-003 and NSC 102-3113-P-006-016 from National Science Council Taiwan . This work was also financed by the Top University Grants (also known as the “5-year-50-billion” Grant) from the Ministry of Education, Taiwan .
PY - 2014/7
Y1 - 2014/7
N2 - A recently isolated thermotolerant microalga Desmodesmus sp. F2 has the traits of becoming potential biodiesel feedstock, such as high growth rate, high lipid content, and quick precipitation. Its overall lipid productivity was 113mg/L/d when grown under non-optimal conditions using batch cultivation. A two-step response surface methodology was adopted to optimize its cultivation conditions. The overall lipid productivity was increased to 263mg/L/d when the cells were grown under the optimized conditions of 6.6mM initial nitrogen level and 6days nitrogen depletion treatment in 700μmol/m2/s light intensity at 35°C using batch cultivation. Fed-batch and semi-continuous cultivations were employed to further increase its lipid productivity to 213 and 302mg/L/d, respectively. The 302mg/L/d is the highest overall lipid productivity of microalgae ever reported in the literature. This study provides the information required for the design and operation of photobioreactors for large scale outdoor cultivation of this species.
AB - A recently isolated thermotolerant microalga Desmodesmus sp. F2 has the traits of becoming potential biodiesel feedstock, such as high growth rate, high lipid content, and quick precipitation. Its overall lipid productivity was 113mg/L/d when grown under non-optimal conditions using batch cultivation. A two-step response surface methodology was adopted to optimize its cultivation conditions. The overall lipid productivity was increased to 263mg/L/d when the cells were grown under the optimized conditions of 6.6mM initial nitrogen level and 6days nitrogen depletion treatment in 700μmol/m2/s light intensity at 35°C using batch cultivation. Fed-batch and semi-continuous cultivations were employed to further increase its lipid productivity to 213 and 302mg/L/d, respectively. The 302mg/L/d is the highest overall lipid productivity of microalgae ever reported in the literature. This study provides the information required for the design and operation of photobioreactors for large scale outdoor cultivation of this species.
UR - https://www.scopus.com/pages/publications/84899886438
UR - https://www.scopus.com/pages/publications/84899886438#tab=citedBy
U2 - 10.1016/j.biortech.2014.04.028
DO - 10.1016/j.biortech.2014.04.028
M3 - Article
C2 - 24796513
AN - SCOPUS:84899886438
SN - 0960-8524
VL - 163
SP - 128
EP - 135
JO - Bioresource technology
JF - Bioresource technology
ER -