Expression and characterization of the chitin-binding domain of chitinase A1 from Bacillus circulans WL-12

Masayuki Hashimoto, Takahisa Ikegami, Shizuka Seino, Nobuhumi Ohuchi, Harumi Fukada, Junji Sugiyama, Masahiro Shirakawa, Takeshi Watanabe

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

Chitinase A1 from Bacillus circulans WL-12 comprises an N-terminal catalytic domain, two fibronectin type III-like domains, and a C-terminal chitin-binding domain (ChBB). In order to study the biochemical properties and structure of the ChBD, ChBD(ChiA1) was produced in Escherichia coli using a pET expression system and purified by chitin affinity column chromatography. Purified ChBD(ChiA1) specifically bound to various forms of insoluble chitin but not to other polysaccharides, including chitosan, cellulose, and starch. Interaction of soluble chitinous substrates with ChBD(ChiA1) was not detected by means of nuclear magnetic resonance and isothermal titration calorimetry. In addition, the presence of soluble substrates did not interfere with the binding of ChBD(ChiA1) to regenerated chitin. These observations suggest that ChBD(ChiA1) recognizes a structure which is present in insoluble or crystalline chitin but not in chito- oligosaccharides or in soluble derivatives of chitin. ChBD(ChiA1) exhibited binding activity over a wide range of pHs, and the binding activity was enhanced at pHs near its pI and by the presence of NaCl, suggesting that the binding of ChBD(ChiA1) is mediated mainly by hydrophobic interactions. Hydrolysis of β-chitin microcrystals by intact chitinase A1 and by a deletion derivative lacking the ChBD suggested that the ChBD is not absolutely required for hydrolysis of β-chitin microcrystals but greatly enhances the efficiency of degradation.

Original languageEnglish
Pages (from-to)3045-3054
Number of pages10
JournalJournal of Bacteriology
Volume182
Issue number11
DOIs
Publication statusPublished - 2000 Jun 1

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Chitinases
Chitin
Bacillus
Hydrolysis
Calorimetry
Chitosan
Oligosaccharides
Hydrophobic and Hydrophilic Interactions
Affinity Chromatography
Cellulose
Starch
Polysaccharides
Catalytic Domain
Magnetic Resonance Spectroscopy
Escherichia coli

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Hashimoto, Masayuki ; Ikegami, Takahisa ; Seino, Shizuka ; Ohuchi, Nobuhumi ; Fukada, Harumi ; Sugiyama, Junji ; Shirakawa, Masahiro ; Watanabe, Takeshi. / Expression and characterization of the chitin-binding domain of chitinase A1 from Bacillus circulans WL-12. In: Journal of Bacteriology. 2000 ; Vol. 182, No. 11. pp. 3045-3054.
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Hashimoto, M, Ikegami, T, Seino, S, Ohuchi, N, Fukada, H, Sugiyama, J, Shirakawa, M & Watanabe, T 2000, 'Expression and characterization of the chitin-binding domain of chitinase A1 from Bacillus circulans WL-12', Journal of Bacteriology, vol. 182, no. 11, pp. 3045-3054. https://doi.org/10.1128/JB.182.11.3045-3054.2000

Expression and characterization of the chitin-binding domain of chitinase A1 from Bacillus circulans WL-12. / Hashimoto, Masayuki; Ikegami, Takahisa; Seino, Shizuka; Ohuchi, Nobuhumi; Fukada, Harumi; Sugiyama, Junji; Shirakawa, Masahiro; Watanabe, Takeshi.

In: Journal of Bacteriology, Vol. 182, No. 11, 01.06.2000, p. 3045-3054.

Research output: Contribution to journalArticle

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T1 - Expression and characterization of the chitin-binding domain of chitinase A1 from Bacillus circulans WL-12

AU - Hashimoto, Masayuki

AU - Ikegami, Takahisa

AU - Seino, Shizuka

AU - Ohuchi, Nobuhumi

AU - Fukada, Harumi

AU - Sugiyama, Junji

AU - Shirakawa, Masahiro

AU - Watanabe, Takeshi

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N2 - Chitinase A1 from Bacillus circulans WL-12 comprises an N-terminal catalytic domain, two fibronectin type III-like domains, and a C-terminal chitin-binding domain (ChBB). In order to study the biochemical properties and structure of the ChBD, ChBD(ChiA1) was produced in Escherichia coli using a pET expression system and purified by chitin affinity column chromatography. Purified ChBD(ChiA1) specifically bound to various forms of insoluble chitin but not to other polysaccharides, including chitosan, cellulose, and starch. Interaction of soluble chitinous substrates with ChBD(ChiA1) was not detected by means of nuclear magnetic resonance and isothermal titration calorimetry. In addition, the presence of soluble substrates did not interfere with the binding of ChBD(ChiA1) to regenerated chitin. These observations suggest that ChBD(ChiA1) recognizes a structure which is present in insoluble or crystalline chitin but not in chito- oligosaccharides or in soluble derivatives of chitin. ChBD(ChiA1) exhibited binding activity over a wide range of pHs, and the binding activity was enhanced at pHs near its pI and by the presence of NaCl, suggesting that the binding of ChBD(ChiA1) is mediated mainly by hydrophobic interactions. Hydrolysis of β-chitin microcrystals by intact chitinase A1 and by a deletion derivative lacking the ChBD suggested that the ChBD is not absolutely required for hydrolysis of β-chitin microcrystals but greatly enhances the efficiency of degradation.

AB - Chitinase A1 from Bacillus circulans WL-12 comprises an N-terminal catalytic domain, two fibronectin type III-like domains, and a C-terminal chitin-binding domain (ChBB). In order to study the biochemical properties and structure of the ChBD, ChBD(ChiA1) was produced in Escherichia coli using a pET expression system and purified by chitin affinity column chromatography. Purified ChBD(ChiA1) specifically bound to various forms of insoluble chitin but not to other polysaccharides, including chitosan, cellulose, and starch. Interaction of soluble chitinous substrates with ChBD(ChiA1) was not detected by means of nuclear magnetic resonance and isothermal titration calorimetry. In addition, the presence of soluble substrates did not interfere with the binding of ChBD(ChiA1) to regenerated chitin. These observations suggest that ChBD(ChiA1) recognizes a structure which is present in insoluble or crystalline chitin but not in chito- oligosaccharides or in soluble derivatives of chitin. ChBD(ChiA1) exhibited binding activity over a wide range of pHs, and the binding activity was enhanced at pHs near its pI and by the presence of NaCl, suggesting that the binding of ChBD(ChiA1) is mediated mainly by hydrophobic interactions. Hydrolysis of β-chitin microcrystals by intact chitinase A1 and by a deletion derivative lacking the ChBD suggested that the ChBD is not absolutely required for hydrolysis of β-chitin microcrystals but greatly enhances the efficiency of degradation.

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