TY - JOUR
T1 - Expression of avian reovirus σC protein in transgenic plants
AU - Huang, Liang Kai
AU - Liao, Sin Chung
AU - Chang, Ching Chun
AU - Liu, Hung Jen
N1 - Funding Information:
The authors would like to thank Mr. Kuo-Chih Wang for technical assistance. This work was financially supported by a grant (NSC90-2311-B-020-002) to Ching-Chun Chang from the National Science Council of Taiwan.
PY - 2006/6
Y1 - 2006/6
N2 - Avian reovirus (ARV) structural protein, σC encoded by S1 genome segment, is the prime candidate to become a vaccine against ARV infection. Two plant nuclear expression vectors with expression of σC-encoding gene driven by CaMV 35S promoter and rice actin promoter were constructed, respectively. Agrobacterium containing the S1 expression constructs were used to transform alfalfa, and transformants were selected using hygromysin. The integration of S1 transgene in alfalfa chromosome was confirmed by PCR and histochemical GUS staining. Western blot analysis using antiserum against σC was carried out to determine the expression of σC protein in transgenic alfalfa cells. The highest expression levels of σC protein in the cellular extracts of selected p35S-S1 and pAct1-S1 transgenic alfalfa lines were 0.008% and 0.007% of the total soluble protein, respectively. The transgenic alfalfa cells with expression of σC protein pave the way for the development of edible vaccine.
AB - Avian reovirus (ARV) structural protein, σC encoded by S1 genome segment, is the prime candidate to become a vaccine against ARV infection. Two plant nuclear expression vectors with expression of σC-encoding gene driven by CaMV 35S promoter and rice actin promoter were constructed, respectively. Agrobacterium containing the S1 expression constructs were used to transform alfalfa, and transformants were selected using hygromysin. The integration of S1 transgene in alfalfa chromosome was confirmed by PCR and histochemical GUS staining. Western blot analysis using antiserum against σC was carried out to determine the expression of σC protein in transgenic alfalfa cells. The highest expression levels of σC protein in the cellular extracts of selected p35S-S1 and pAct1-S1 transgenic alfalfa lines were 0.008% and 0.007% of the total soluble protein, respectively. The transgenic alfalfa cells with expression of σC protein pave the way for the development of edible vaccine.
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U2 - 10.1016/j.jviromet.2006.01.013
DO - 10.1016/j.jviromet.2006.01.013
M3 - Article
C2 - 16488486
AN - SCOPUS:33646149978
SN - 0166-0934
VL - 134
SP - 217
EP - 222
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 1-2
ER -
