Expression of chimeric monomer and dimer proteins on the plasma membrane of mammalian cells

Wan Chih Chou, Kuang Wen Liao, Yu Chih Lo, Shu Yaun Jiang, Ming Yang Yeh, Steve R. Roffler

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Targeting of proteins to the plasma membrane of cells may be useful for vaccine development, tissue engineering, genetic research, bioseparations, and disease treatment. The ability of different transmembrane domains (TM) to direct a reporter protein (human alphafeto protein, AFP) to the surface of mammalian cells was examined. High surface expression was achieved with chimeric proteins composed of AFP and the TM and cytosolic tail of murine B7- 1 (AFP-B7) as well as with AFP containing a GPI-anchor from decay- accelerating factor (AFP-DAF). Lower surface expression of AFP was observed when the TM of human platelet-derived growth factor receptor or the human asialoglycoprotein receptor H1 subunit were employed. Introduction of the hingeCH2-CH3 region of human IgG (γ1 domain) between AFP and TM allowed efficient formation of disulfide-linked dimers. Surface expression of AFP- γ1-B7 dimers was impaired compared to AFP-B7 whereas AFPγ 1-DAF dimers were efficiently targeted to the surface. Accumulation of chimeric proteins on the cell surface did not correlate with the level of protein expression. This study demonstrates that high levels of monomeric and dimeric proteins can be targeted to the cell membrane of mammalian cells by proper selection of TM.

Original languageEnglish
Pages (from-to)160-169
Number of pages10
JournalBiotechnology and Bioengineering
Volume65
Issue number2
DOIs
Publication statusPublished - 1999 Oct 20

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Expression of chimeric monomer and dimer proteins on the plasma membrane of mammalian cells'. Together they form a unique fingerprint.

Cite this