Expression of cyclin-dependent kinase 2-associated protein 1 confers an independent prognosticator in nasopharyngeal carcinoma: A cohort study

Li Ching Wu, Yi Ling Chen, Wen Ren Wu, Chien Feng Li, Hsuan Ying Huang, Sung Wei Lee, Shih Lun Chang, Ching Yih Lin, Yi Hsien Chen, Han Ping Hsu, Pei Jung Lu, Yow Ling Shiue

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background and aim: Low expression of cyclin-dependent kinase 2-associated protein (CDK2AP1) is associated with tumour progression in oral and oesophageal carcinomas, but is not well studied in patients with head and neck cancer and nasopharyngeal carcinoma (NPC). Methods: A rabbit anti-human CDK2AP1 polyclonal antibody was prepared. Immunoblotting of CDK2AP1 was examined in three cell lines and immunoexpression was retrospectively assessed in biopsies of 124 consecutive NPC patients without initial distant metastasis and treated with consistent guidelines. Results: Higher CDK2AP1 expression level was identified in dysplastic oral keratinocytes, compared with two NPC-derived HONE-1 and TW01 cell lines. Low expression of CDK2AP1 (50.8%) was correlated with advanced nodal status (p=0.002) and American Joint Committee on Cancer (AJCC) stage (p=0.004). In multivariate analyses, low CDK2AP1 expression emerged as an independent prognosticator for worse disease-specific survival (DSS; p=0.037) and local recurrence-free survival (LRFS; p=0.042), along with AJCC stage III-IV (p=0.034, DSS; p=0.029, LRFS). Conclusions: Low CDK2AP1 expression is common and associated with adverse prognosticators, conferring tumour aggressiveness through cycle cycle, cell growth or apoptosis cellular processes.

Original languageEnglish
Pages (from-to)795-801
Number of pages7
JournalJournal of Clinical Pathology
Volume65
Issue number9
DOIs
Publication statusPublished - 2012 Sep 1

Fingerprint

Cyclin-Dependent Kinase 2
Cohort Studies
Neoplasms
Proteins
Cell Line
Survival
Head and Neck Neoplasms
Keratinocytes
Immunoblotting
Cell Cycle
Multivariate Analysis
Guidelines
Apoptosis
Neoplasm Metastasis
Rabbits
Carcinoma
Biopsy
Recurrence
Nasopharyngeal carcinoma
Antibodies

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Wu, Li Ching ; Chen, Yi Ling ; Wu, Wen Ren ; Li, Chien Feng ; Huang, Hsuan Ying ; Lee, Sung Wei ; Chang, Shih Lun ; Lin, Ching Yih ; Chen, Yi Hsien ; Hsu, Han Ping ; Lu, Pei Jung ; Shiue, Yow Ling. / Expression of cyclin-dependent kinase 2-associated protein 1 confers an independent prognosticator in nasopharyngeal carcinoma : A cohort study. In: Journal of Clinical Pathology. 2012 ; Vol. 65, No. 9. pp. 795-801.
@article{5885641246844a599cff3d0b51c56326,
title = "Expression of cyclin-dependent kinase 2-associated protein 1 confers an independent prognosticator in nasopharyngeal carcinoma: A cohort study",
abstract = "Background and aim: Low expression of cyclin-dependent kinase 2-associated protein (CDK2AP1) is associated with tumour progression in oral and oesophageal carcinomas, but is not well studied in patients with head and neck cancer and nasopharyngeal carcinoma (NPC). Methods: A rabbit anti-human CDK2AP1 polyclonal antibody was prepared. Immunoblotting of CDK2AP1 was examined in three cell lines and immunoexpression was retrospectively assessed in biopsies of 124 consecutive NPC patients without initial distant metastasis and treated with consistent guidelines. Results: Higher CDK2AP1 expression level was identified in dysplastic oral keratinocytes, compared with two NPC-derived HONE-1 and TW01 cell lines. Low expression of CDK2AP1 (50.8{\%}) was correlated with advanced nodal status (p=0.002) and American Joint Committee on Cancer (AJCC) stage (p=0.004). In multivariate analyses, low CDK2AP1 expression emerged as an independent prognosticator for worse disease-specific survival (DSS; p=0.037) and local recurrence-free survival (LRFS; p=0.042), along with AJCC stage III-IV (p=0.034, DSS; p=0.029, LRFS). Conclusions: Low CDK2AP1 expression is common and associated with adverse prognosticators, conferring tumour aggressiveness through cycle cycle, cell growth or apoptosis cellular processes.",
author = "Wu, {Li Ching} and Chen, {Yi Ling} and Wu, {Wen Ren} and Li, {Chien Feng} and Huang, {Hsuan Ying} and Lee, {Sung Wei} and Chang, {Shih Lun} and Lin, {Ching Yih} and Chen, {Yi Hsien} and Hsu, {Han Ping} and Lu, {Pei Jung} and Shiue, {Yow Ling}",
year = "2012",
month = "9",
day = "1",
doi = "10.1136/jclinpath-2012-200893",
language = "English",
volume = "65",
pages = "795--801",
journal = "Journal of Clinical Pathology",
issn = "0021-9746",
publisher = "BMJ Publishing Group",
number = "9",

}

Expression of cyclin-dependent kinase 2-associated protein 1 confers an independent prognosticator in nasopharyngeal carcinoma : A cohort study. / Wu, Li Ching; Chen, Yi Ling; Wu, Wen Ren; Li, Chien Feng; Huang, Hsuan Ying; Lee, Sung Wei; Chang, Shih Lun; Lin, Ching Yih; Chen, Yi Hsien; Hsu, Han Ping; Lu, Pei Jung; Shiue, Yow Ling.

In: Journal of Clinical Pathology, Vol. 65, No. 9, 01.09.2012, p. 795-801.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of cyclin-dependent kinase 2-associated protein 1 confers an independent prognosticator in nasopharyngeal carcinoma

T2 - A cohort study

AU - Wu, Li Ching

AU - Chen, Yi Ling

AU - Wu, Wen Ren

AU - Li, Chien Feng

AU - Huang, Hsuan Ying

AU - Lee, Sung Wei

AU - Chang, Shih Lun

AU - Lin, Ching Yih

AU - Chen, Yi Hsien

AU - Hsu, Han Ping

AU - Lu, Pei Jung

AU - Shiue, Yow Ling

PY - 2012/9/1

Y1 - 2012/9/1

N2 - Background and aim: Low expression of cyclin-dependent kinase 2-associated protein (CDK2AP1) is associated with tumour progression in oral and oesophageal carcinomas, but is not well studied in patients with head and neck cancer and nasopharyngeal carcinoma (NPC). Methods: A rabbit anti-human CDK2AP1 polyclonal antibody was prepared. Immunoblotting of CDK2AP1 was examined in three cell lines and immunoexpression was retrospectively assessed in biopsies of 124 consecutive NPC patients without initial distant metastasis and treated with consistent guidelines. Results: Higher CDK2AP1 expression level was identified in dysplastic oral keratinocytes, compared with two NPC-derived HONE-1 and TW01 cell lines. Low expression of CDK2AP1 (50.8%) was correlated with advanced nodal status (p=0.002) and American Joint Committee on Cancer (AJCC) stage (p=0.004). In multivariate analyses, low CDK2AP1 expression emerged as an independent prognosticator for worse disease-specific survival (DSS; p=0.037) and local recurrence-free survival (LRFS; p=0.042), along with AJCC stage III-IV (p=0.034, DSS; p=0.029, LRFS). Conclusions: Low CDK2AP1 expression is common and associated with adverse prognosticators, conferring tumour aggressiveness through cycle cycle, cell growth or apoptosis cellular processes.

AB - Background and aim: Low expression of cyclin-dependent kinase 2-associated protein (CDK2AP1) is associated with tumour progression in oral and oesophageal carcinomas, but is not well studied in patients with head and neck cancer and nasopharyngeal carcinoma (NPC). Methods: A rabbit anti-human CDK2AP1 polyclonal antibody was prepared. Immunoblotting of CDK2AP1 was examined in three cell lines and immunoexpression was retrospectively assessed in biopsies of 124 consecutive NPC patients without initial distant metastasis and treated with consistent guidelines. Results: Higher CDK2AP1 expression level was identified in dysplastic oral keratinocytes, compared with two NPC-derived HONE-1 and TW01 cell lines. Low expression of CDK2AP1 (50.8%) was correlated with advanced nodal status (p=0.002) and American Joint Committee on Cancer (AJCC) stage (p=0.004). In multivariate analyses, low CDK2AP1 expression emerged as an independent prognosticator for worse disease-specific survival (DSS; p=0.037) and local recurrence-free survival (LRFS; p=0.042), along with AJCC stage III-IV (p=0.034, DSS; p=0.029, LRFS). Conclusions: Low CDK2AP1 expression is common and associated with adverse prognosticators, conferring tumour aggressiveness through cycle cycle, cell growth or apoptosis cellular processes.

UR - http://www.scopus.com/inward/record.url?scp=84866102917&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84866102917&partnerID=8YFLogxK

U2 - 10.1136/jclinpath-2012-200893

DO - 10.1136/jclinpath-2012-200893

M3 - Article

C2 - 22791769

AN - SCOPUS:84866102917

VL - 65

SP - 795

EP - 801

JO - Journal of Clinical Pathology

JF - Journal of Clinical Pathology

SN - 0021-9746

IS - 9

ER -