Expression of DAZL protein in the human corpus luteum

H. A. Pan, Shaw-Jenq Tsai, C. W. Chen, Y. C. Lee, YungMing Lin, Pao-Lin Kuo

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Abstract

The DAZL gene and its homologues are required for the development of male and female germ cells in different species. However, their role in other aspects of human reproduction is not known. We have generated a polyclonal antibody to the DAZL protein and developed a sensitive standard curve quantitative-competitive-RT-PCR assay to characterize the expression of DAZL in the human corpus luteum (CL). DAZL transcripts are expressed in the CL, but the concentrations decreased with advancing luteal phase. In accordance with the mRNA data, DAZL protein was most abundant in the early phase CL. Immunohistochemical staining showed DAZL protein in the cytoplasm of granulosa-luteal cells. The distinct expression pattern of DAZL protein in the human CL may play an important role in the regulation of luteal function.

Original languageEnglish
Pages (from-to)540-545
Number of pages6
JournalMolecular Human Reproduction
Volume8
Issue number6
Publication statusPublished - 2002 Jul 20

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All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Embryology
  • Molecular Biology
  • Genetics
  • Obstetrics and Gynaecology
  • Developmental Biology
  • Cell Biology

Cite this

Pan, H. A., Tsai, S-J., Chen, C. W., Lee, Y. C., Lin, Y., & Kuo, P-L. (2002). Expression of DAZL protein in the human corpus luteum. Molecular Human Reproduction, 8(6), 540-545.