TY - JOUR
T1 - Expression of EBV-encoded oncogenes and EBV-like virions in multiple canine tumors
AU - Chiu, Hung Chuan
AU - Chow, Kuan Chih
AU - Fan, Yi Hsin
AU - Chang, Shih Chieh
AU - Chiou, Shiow Her
AU - Chiang, Shu Fen
AU - Chiou, Che Hao
AU - Wu, Guo Hua
AU - Yang, Hsiu Ching
AU - Ho, Shu Peng
AU - Chen, Yuh Kun
AU - Lee, Wei Cheng
AU - Sun, H. Sunny
N1 - Funding Information:
We thank Pei-Shan Wu, Ting-Er Lin, Chun-Hua Huang, and Che-Yuan Chang for their technical assistance. We thank Pei-Chi Chao for her assistance in thin sectioning and electron microscope operation. We thank Dr. Yong-Sheng Zong for kindly providing us with CNE2 cells. This work was supported by the National Science Council, Taiwan, ROC (grant numbers NSC95-2313-B-005-033-MY2 , NSC97-2321-B-005-015 , NSC98-2313-B-005-012 , and NSC99-2313-B-005-010-MY3 ) and the Ministry of Education, Taiwan, ROC (ATU plan).
PY - 2013
Y1 - 2013
N2 - Epstein-Barr virus (EBV) is a ubiquitous human oncovirus. Previous studies by us and others have indicated that pet dogs frequently encounter EBV or EBV-related viral infection. In this study, we explored whether EBV is involved in canine malignancies in dogs. EBV-specific BamHI W sequence was detected by polymerase chain reaction (PCR) in 10 of 12 canine tumor specimens, including 8 of 10 oral tumors. Using reverse transcription-PCR, gene expressions of latent membrane protein 1 (LMP 1) and BamHI H rightward reading frame 1 (BHRF1) were identified in 8 and 7 of 12 specimens, respectively. A novel LMP1 variant, T0905, was predominant in 5 canine tumor specimens and found to exist in EBV positive human BC-2 cells. Another LMP1 variant, T0902, was similar to human tumor variant JB7. The BHRF1 sequence identified from these canine tumors was identical to that of the B95-8 viral strain. LMP1 protein and EBV-encoded RNA (EBER) were detected by immunohistochemistry and fluorescent in situ hybridization, respectively, in several tumors, particularly in tumor nests of oral amelanotic melanomas. Furthermore, EBV-like virions adopting a herpesvirus egress pathway were detected in a canthal fibroblastic osteosarcoma and an oral amelanotic melanoma.In conclusion, we report the expressions of BHRF1 transcript (a viral anti-apoptotic protein), LMP1 (a viral oncoprotein) transcript and protein, EBER (a viral oncogenic RNA), and EBV-like virions in multiple canine tumors. The identity of BHRF1 and the resemblance of LMP1 variants between canine and human tumors indicate either a close evolutionary relationship between canine and human EBV, or the possibility of zoonotic transmission.
AB - Epstein-Barr virus (EBV) is a ubiquitous human oncovirus. Previous studies by us and others have indicated that pet dogs frequently encounter EBV or EBV-related viral infection. In this study, we explored whether EBV is involved in canine malignancies in dogs. EBV-specific BamHI W sequence was detected by polymerase chain reaction (PCR) in 10 of 12 canine tumor specimens, including 8 of 10 oral tumors. Using reverse transcription-PCR, gene expressions of latent membrane protein 1 (LMP 1) and BamHI H rightward reading frame 1 (BHRF1) were identified in 8 and 7 of 12 specimens, respectively. A novel LMP1 variant, T0905, was predominant in 5 canine tumor specimens and found to exist in EBV positive human BC-2 cells. Another LMP1 variant, T0902, was similar to human tumor variant JB7. The BHRF1 sequence identified from these canine tumors was identical to that of the B95-8 viral strain. LMP1 protein and EBV-encoded RNA (EBER) were detected by immunohistochemistry and fluorescent in situ hybridization, respectively, in several tumors, particularly in tumor nests of oral amelanotic melanomas. Furthermore, EBV-like virions adopting a herpesvirus egress pathway were detected in a canthal fibroblastic osteosarcoma and an oral amelanotic melanoma.In conclusion, we report the expressions of BHRF1 transcript (a viral anti-apoptotic protein), LMP1 (a viral oncoprotein) transcript and protein, EBER (a viral oncogenic RNA), and EBV-like virions in multiple canine tumors. The identity of BHRF1 and the resemblance of LMP1 variants between canine and human tumors indicate either a close evolutionary relationship between canine and human EBV, or the possibility of zoonotic transmission.
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U2 - 10.1016/j.vetmic.2012.12.022
DO - 10.1016/j.vetmic.2012.12.022
M3 - Article
C2 - 23380461
AN - SCOPUS:84874578035
VL - 163
SP - 79
EP - 89
JO - Veterinary Microbiology
JF - Veterinary Microbiology
SN - 0378-1135
IS - 1-2
ER -