Expression of EBV-encoded oncogenes and EBV-like virions in multiple canine tumors

Hung Chuan Chiu, Kuan Chih Chow, Yi Hsin Fan, Shih Chieh Chang, Shiow Her Chiou, Shu Fen Chiang, Che Hao Chiou, Guo Hua Wu, Hsiu Ching Yang, Shu Peng Ho, Yuh Kun Chen, Wei Cheng Lee, Hsiao-Fang Sun

Research output: Contribution to journalArticle

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Abstract

Epstein-Barr virus (EBV) is a ubiquitous human oncovirus. Previous studies by us and others have indicated that pet dogs frequently encounter EBV or EBV-related viral infection. In this study, we explored whether EBV is involved in canine malignancies in dogs. EBV-specific BamHI W sequence was detected by polymerase chain reaction (PCR) in 10 of 12 canine tumor specimens, including 8 of 10 oral tumors. Using reverse transcription-PCR, gene expressions of latent membrane protein 1 (LMP 1) and BamHI H rightward reading frame 1 (BHRF1) were identified in 8 and 7 of 12 specimens, respectively. A novel LMP1 variant, T0905, was predominant in 5 canine tumor specimens and found to exist in EBV positive human BC-2 cells. Another LMP1 variant, T0902, was similar to human tumor variant JB7. The BHRF1 sequence identified from these canine tumors was identical to that of the B95-8 viral strain. LMP1 protein and EBV-encoded RNA (EBER) were detected by immunohistochemistry and fluorescent in situ hybridization, respectively, in several tumors, particularly in tumor nests of oral amelanotic melanomas. Furthermore, EBV-like virions adopting a herpesvirus egress pathway were detected in a canthal fibroblastic osteosarcoma and an oral amelanotic melanoma.In conclusion, we report the expressions of BHRF1 transcript (a viral anti-apoptotic protein), LMP1 (a viral oncoprotein) transcript and protein, EBER (a viral oncogenic RNA), and EBV-like virions in multiple canine tumors. The identity of BHRF1 and the resemblance of LMP1 variants between canine and human tumors indicate either a close evolutionary relationship between canine and human EBV, or the possibility of zoonotic transmission.

Original languageEnglish
Pages (from-to)79-89
Number of pages11
JournalVeterinary Microbiology
Volume163
Issue number1-2
DOIs
Publication statusPublished - 2013 Feb 4

Fingerprint

Human herpesvirus 4
oncogenes
Human Herpesvirus 4
virion
Oncogenes
Virion
Canidae
neoplasms
dogs
Reading Frames
Neoplasms
Amelanotic Melanoma
mouth
RNA
melanoma
Betaretrovirus
Dogs
osteosarcoma
Polymerase Chain Reaction
Apoptosis Regulatory Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • veterinary(all)

Cite this

Chiu, Hung Chuan ; Chow, Kuan Chih ; Fan, Yi Hsin ; Chang, Shih Chieh ; Chiou, Shiow Her ; Chiang, Shu Fen ; Chiou, Che Hao ; Wu, Guo Hua ; Yang, Hsiu Ching ; Ho, Shu Peng ; Chen, Yuh Kun ; Lee, Wei Cheng ; Sun, Hsiao-Fang. / Expression of EBV-encoded oncogenes and EBV-like virions in multiple canine tumors. In: Veterinary Microbiology. 2013 ; Vol. 163, No. 1-2. pp. 79-89.
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abstract = "Epstein-Barr virus (EBV) is a ubiquitous human oncovirus. Previous studies by us and others have indicated that pet dogs frequently encounter EBV or EBV-related viral infection. In this study, we explored whether EBV is involved in canine malignancies in dogs. EBV-specific BamHI W sequence was detected by polymerase chain reaction (PCR) in 10 of 12 canine tumor specimens, including 8 of 10 oral tumors. Using reverse transcription-PCR, gene expressions of latent membrane protein 1 (LMP 1) and BamHI H rightward reading frame 1 (BHRF1) were identified in 8 and 7 of 12 specimens, respectively. A novel LMP1 variant, T0905, was predominant in 5 canine tumor specimens and found to exist in EBV positive human BC-2 cells. Another LMP1 variant, T0902, was similar to human tumor variant JB7. The BHRF1 sequence identified from these canine tumors was identical to that of the B95-8 viral strain. LMP1 protein and EBV-encoded RNA (EBER) were detected by immunohistochemistry and fluorescent in situ hybridization, respectively, in several tumors, particularly in tumor nests of oral amelanotic melanomas. Furthermore, EBV-like virions adopting a herpesvirus egress pathway were detected in a canthal fibroblastic osteosarcoma and an oral amelanotic melanoma.In conclusion, we report the expressions of BHRF1 transcript (a viral anti-apoptotic protein), LMP1 (a viral oncoprotein) transcript and protein, EBER (a viral oncogenic RNA), and EBV-like virions in multiple canine tumors. The identity of BHRF1 and the resemblance of LMP1 variants between canine and human tumors indicate either a close evolutionary relationship between canine and human EBV, or the possibility of zoonotic transmission.",
author = "Chiu, {Hung Chuan} and Chow, {Kuan Chih} and Fan, {Yi Hsin} and Chang, {Shih Chieh} and Chiou, {Shiow Her} and Chiang, {Shu Fen} and Chiou, {Che Hao} and Wu, {Guo Hua} and Yang, {Hsiu Ching} and Ho, {Shu Peng} and Chen, {Yuh Kun} and Lee, {Wei Cheng} and Hsiao-Fang Sun",
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Chiu, HC, Chow, KC, Fan, YH, Chang, SC, Chiou, SH, Chiang, SF, Chiou, CH, Wu, GH, Yang, HC, Ho, SP, Chen, YK, Lee, WC & Sun, H-F 2013, 'Expression of EBV-encoded oncogenes and EBV-like virions in multiple canine tumors', Veterinary Microbiology, vol. 163, no. 1-2, pp. 79-89. https://doi.org/10.1016/j.vetmic.2012.12.022

Expression of EBV-encoded oncogenes and EBV-like virions in multiple canine tumors. / Chiu, Hung Chuan; Chow, Kuan Chih; Fan, Yi Hsin; Chang, Shih Chieh; Chiou, Shiow Her; Chiang, Shu Fen; Chiou, Che Hao; Wu, Guo Hua; Yang, Hsiu Ching; Ho, Shu Peng; Chen, Yuh Kun; Lee, Wei Cheng; Sun, Hsiao-Fang.

In: Veterinary Microbiology, Vol. 163, No. 1-2, 04.02.2013, p. 79-89.

Research output: Contribution to journalArticle

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AU - Chiu, Hung Chuan

AU - Chow, Kuan Chih

AU - Fan, Yi Hsin

AU - Chang, Shih Chieh

AU - Chiou, Shiow Her

AU - Chiang, Shu Fen

AU - Chiou, Che Hao

AU - Wu, Guo Hua

AU - Yang, Hsiu Ching

AU - Ho, Shu Peng

AU - Chen, Yuh Kun

AU - Lee, Wei Cheng

AU - Sun, Hsiao-Fang

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