Expression of genes encoding the rice translation initiation factor, eIF5A, is involved in developmental and environmental responses

Wan Chi Chou, Ya Wen Huang, Wen Su Tsay, Tzen Yuh Chiang, Dinq Ding Huang, Hao Jen Huang

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

Eukaryotic translation initiation factor 5 A (eIF5A) is the only cellular protein known to contain the unusual amino acid hypusine. However, the precise cellular function of eIF5A is to date unknown. In the present study, we report on the characterization of two cDNA clones encoding eIF5A in rice (Oryza sativa). Sequence analysis revealed that the two cDNAs share 93% amino acid sequence identity. Phylogenetic analysis of the eIF5A genes revealed paraphyly of OseIF5A-1 and OseIF5A-2. Analysis at the mRNA level has shown that OseIF5A-1 and OseIF5A-2 are expressed in rice leaves and panicles and high relative amounts of both genes were detected in old leaves. In addition, both OseIF5A-1 and OseIF5A-2 were spatially regulated during rice leaf development. In suspension-cultured cells, the transcripts of OseIF5A genes were strongly reduced after sugar starvation. Abiotic stresses, salt and heavy metal, induce the accumulation of OseIF5A-1 and OseIF5A-2 mRNAs in rice cells. These results suggested that both OseIF5A genes might be regulated by plant development and environmental stresses.

Original languageEnglish
Pages (from-to)50-57
Number of pages8
JournalPhysiologia plantarum
Volume121
Issue number1
DOIs
Publication statusPublished - 2004 May 1

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

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