Expression of sex-determining genes in human sebaceous glands and their possible role in the pathogenesis of acne

W. Chen, Chao-Chun Yang, C. L. Liao, C. L. Hung, Shaw-Jenq Tsai, K. F. Chen, H. M. Sheu, C. C. Zouboulis

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background: The human skin, especially the sebaceous gland, is a steroidogenic organ similar to the gonads and adrenal cortex, possessing all the enzymes required for steroid sex-hormone synthesis and metabolism. Factors regulating cutaneous steroidogenesis associated with disease status remain largely unknown. Objective: We hypothesized that transcription factors involved in sex formation and regulation of steroidogenesis in the classical steroidogenic organs are also expressed in the sebaceous glands. Their possible role in the pathogenesis of acne were investigated. Methods: We used reverse transcription polymerase chain reaction (RT-PCR), in situ hybridization and Western blotting to analyse the expression of SF-1, WT-1, SRY, SOX-9 and DAX-1 mRNAs and their proteins in cultured human sebocytes and the facial skin of acne patients. Results: The in situ hybridization study showed SOX-9 mRNA mainly localized in basal keratinocytes, the basal layer of the sebaceous glands and eccrine glands. Immortalized human sebaceous gland cells (SZ95) expressed mRNA for SOX-9, WT-1 and DAX-1 but not for SF-1 or SRY. The expression of DAX-1 protein was slightly inhibited by 10 -6 m oestradiol (E2) at 6 h but enhanced by 10 -6 m dihydrotestosterone (DHT) at 48 h. The facial expression of SOX-9 seemed to be higher in the acne-prone male patients, while DAX-1 was stronger in subjects without acne, although both were statistically insignificant. Conclusion: Our findings confirm the expression of some sex-determining genes in human sebaceous glands. Further studies on a larger patient population including the normal controls are needed to elucidate the functional significance of these transcription factors in the pathogenesis of acne.

Original languageEnglish
Pages (from-to)846-852
Number of pages7
JournalJournal of the European Academy of Dermatology and Venereology
Volume20
Issue number7
DOIs
Publication statusPublished - 2006 Aug 1

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Sebaceous Glands
Acne Vulgaris
Genes
Messenger RNA
Skin
In Situ Hybridization
Transcription Factors
Eccrine Glands
Facial Expression
Dihydrotestosterone
Adrenal Cortex
Gonads
Gonadal Steroid Hormones
Keratinocytes
Reverse Transcription
Estradiol
Proteins
Western Blotting
Polymerase Chain Reaction
Enzymes

All Science Journal Classification (ASJC) codes

  • Dermatology
  • Infectious Diseases

Cite this

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title = "Expression of sex-determining genes in human sebaceous glands and their possible role in the pathogenesis of acne",
abstract = "Background: The human skin, especially the sebaceous gland, is a steroidogenic organ similar to the gonads and adrenal cortex, possessing all the enzymes required for steroid sex-hormone synthesis and metabolism. Factors regulating cutaneous steroidogenesis associated with disease status remain largely unknown. Objective: We hypothesized that transcription factors involved in sex formation and regulation of steroidogenesis in the classical steroidogenic organs are also expressed in the sebaceous glands. Their possible role in the pathogenesis of acne were investigated. Methods: We used reverse transcription polymerase chain reaction (RT-PCR), in situ hybridization and Western blotting to analyse the expression of SF-1, WT-1, SRY, SOX-9 and DAX-1 mRNAs and their proteins in cultured human sebocytes and the facial skin of acne patients. Results: The in situ hybridization study showed SOX-9 mRNA mainly localized in basal keratinocytes, the basal layer of the sebaceous glands and eccrine glands. Immortalized human sebaceous gland cells (SZ95) expressed mRNA for SOX-9, WT-1 and DAX-1 but not for SF-1 or SRY. The expression of DAX-1 protein was slightly inhibited by 10 -6 m oestradiol (E2) at 6 h but enhanced by 10 -6 m dihydrotestosterone (DHT) at 48 h. The facial expression of SOX-9 seemed to be higher in the acne-prone male patients, while DAX-1 was stronger in subjects without acne, although both were statistically insignificant. Conclusion: Our findings confirm the expression of some sex-determining genes in human sebaceous glands. Further studies on a larger patient population including the normal controls are needed to elucidate the functional significance of these transcription factors in the pathogenesis of acne.",
author = "W. Chen and Chao-Chun Yang and Liao, {C. L.} and Hung, {C. L.} and Shaw-Jenq Tsai and Chen, {K. F.} and Sheu, {H. M.} and Zouboulis, {C. C.}",
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Expression of sex-determining genes in human sebaceous glands and their possible role in the pathogenesis of acne. / Chen, W.; Yang, Chao-Chun; Liao, C. L.; Hung, C. L.; Tsai, Shaw-Jenq; Chen, K. F.; Sheu, H. M.; Zouboulis, C. C.

In: Journal of the European Academy of Dermatology and Venereology, Vol. 20, No. 7, 01.08.2006, p. 846-852.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Yang, Chao-Chun

AU - Liao, C. L.

AU - Hung, C. L.

AU - Tsai, Shaw-Jenq

AU - Chen, K. F.

AU - Sheu, H. M.

AU - Zouboulis, C. C.

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N2 - Background: The human skin, especially the sebaceous gland, is a steroidogenic organ similar to the gonads and adrenal cortex, possessing all the enzymes required for steroid sex-hormone synthesis and metabolism. Factors regulating cutaneous steroidogenesis associated with disease status remain largely unknown. Objective: We hypothesized that transcription factors involved in sex formation and regulation of steroidogenesis in the classical steroidogenic organs are also expressed in the sebaceous glands. Their possible role in the pathogenesis of acne were investigated. Methods: We used reverse transcription polymerase chain reaction (RT-PCR), in situ hybridization and Western blotting to analyse the expression of SF-1, WT-1, SRY, SOX-9 and DAX-1 mRNAs and their proteins in cultured human sebocytes and the facial skin of acne patients. Results: The in situ hybridization study showed SOX-9 mRNA mainly localized in basal keratinocytes, the basal layer of the sebaceous glands and eccrine glands. Immortalized human sebaceous gland cells (SZ95) expressed mRNA for SOX-9, WT-1 and DAX-1 but not for SF-1 or SRY. The expression of DAX-1 protein was slightly inhibited by 10 -6 m oestradiol (E2) at 6 h but enhanced by 10 -6 m dihydrotestosterone (DHT) at 48 h. The facial expression of SOX-9 seemed to be higher in the acne-prone male patients, while DAX-1 was stronger in subjects without acne, although both were statistically insignificant. Conclusion: Our findings confirm the expression of some sex-determining genes in human sebaceous glands. Further studies on a larger patient population including the normal controls are needed to elucidate the functional significance of these transcription factors in the pathogenesis of acne.

AB - Background: The human skin, especially the sebaceous gland, is a steroidogenic organ similar to the gonads and adrenal cortex, possessing all the enzymes required for steroid sex-hormone synthesis and metabolism. Factors regulating cutaneous steroidogenesis associated with disease status remain largely unknown. Objective: We hypothesized that transcription factors involved in sex formation and regulation of steroidogenesis in the classical steroidogenic organs are also expressed in the sebaceous glands. Their possible role in the pathogenesis of acne were investigated. Methods: We used reverse transcription polymerase chain reaction (RT-PCR), in situ hybridization and Western blotting to analyse the expression of SF-1, WT-1, SRY, SOX-9 and DAX-1 mRNAs and their proteins in cultured human sebocytes and the facial skin of acne patients. Results: The in situ hybridization study showed SOX-9 mRNA mainly localized in basal keratinocytes, the basal layer of the sebaceous glands and eccrine glands. Immortalized human sebaceous gland cells (SZ95) expressed mRNA for SOX-9, WT-1 and DAX-1 but not for SF-1 or SRY. The expression of DAX-1 protein was slightly inhibited by 10 -6 m oestradiol (E2) at 6 h but enhanced by 10 -6 m dihydrotestosterone (DHT) at 48 h. The facial expression of SOX-9 seemed to be higher in the acne-prone male patients, while DAX-1 was stronger in subjects without acne, although both were statistically insignificant. Conclusion: Our findings confirm the expression of some sex-determining genes in human sebaceous glands. Further studies on a larger patient population including the normal controls are needed to elucidate the functional significance of these transcription factors in the pathogenesis of acne.

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