Expression of snake venom toxins in Pichia pastoris

Raveendra Anangi, Chiu Yueh Chen, Chun Ho Cheng, Yi Chun Chen, Chu Chun Chen, Yuan Ping Chu, Chia Hao Chang, Wen Yih Jeng, Jia Hau Shiu, Woei Jer Chuang

Research output: Contribution to journalReview article

13 Citations (Scopus)

Abstract

Snake venom is a cocktail of hundreds to thousands of biologically active proteins and enzymes used in both attack and defense. Because these proteins are capable of modulating the physiological response of envenomated animals, they show promise as potential pharmacological tools and as drug leads. In order to study function and structure relationships of snake venom proteins, many protein expression systems, including E. coli, yeast, baculovirus infected insect cells, mammalian cells, and cell-free protein synthesis, were used to produce recombinant proteins. Many venom proteins cannot be expressed in E. coli with the correct fold because most of them are highly disulfide-bonded. In contrast, many proteins have been successfully expressed in eukaryotic systems. Even though eukaryotic expression systems may allow the expression of proteins with the correct fold, they have certain disadvantages. For example, most proteins obtained from these expression systems are expensive, time consuming to produce, and are in low yield. Recently the methylotrophic yeast Pichia pastoris has drawn a lot of attention because it combines several advantages of prokaryotic and eukaryotic expression systems. It is the only system that offers the quality of E. coli like high level expression, easy scale up, and inexpensive growth medium combined with the advantages of expression in a eukaryotic system (protein processing, folding, and posttranslational modifications). Therefore, the P. pastoris expression system has been used to produce a variety of heterologous proteins. The P. pastoris system has also been widely used to express many snake venom proteins. The focus of this review will summarize the results of snake venom toxins expressed in P. pastoris.

Original languageEnglish
Pages (from-to)169-187
Number of pages19
JournalToxin Reviews
Volume26
Issue number2
DOIs
Publication statusPublished - 2007 Apr 1

Fingerprint

Snake Venoms
Pichia
Proteins
Escherichia coli
Yeast
Yeasts
Baculoviridae
Protein Folding
Venoms
Post Translational Protein Processing
Recombinant Proteins
Disulfides
Insects
Animals
Pharmacology
Cells

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Anangi, Raveendra ; Chen, Chiu Yueh ; Cheng, Chun Ho ; Chen, Yi Chun ; Chen, Chu Chun ; Chu, Yuan Ping ; Chang, Chia Hao ; Jeng, Wen Yih ; Shiu, Jia Hau ; Chuang, Woei Jer. / Expression of snake venom toxins in Pichia pastoris. In: Toxin Reviews. 2007 ; Vol. 26, No. 2. pp. 169-187.
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Anangi, R, Chen, CY, Cheng, CH, Chen, YC, Chen, CC, Chu, YP, Chang, CH, Jeng, WY, Shiu, JH & Chuang, WJ 2007, 'Expression of snake venom toxins in Pichia pastoris', Toxin Reviews, vol. 26, no. 2, pp. 169-187. https://doi.org/10.1080/15569540701209815

Expression of snake venom toxins in Pichia pastoris. / Anangi, Raveendra; Chen, Chiu Yueh; Cheng, Chun Ho; Chen, Yi Chun; Chen, Chu Chun; Chu, Yuan Ping; Chang, Chia Hao; Jeng, Wen Yih; Shiu, Jia Hau; Chuang, Woei Jer.

In: Toxin Reviews, Vol. 26, No. 2, 01.04.2007, p. 169-187.

Research output: Contribution to journalReview article

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