Expression of Synthetic Phytoene Synthase Gene to Enhance β-Carotene Production in Scenedesmus sp. CPC2

Chun Yen Chen, Ai Ling Kao, Zheng Chia Tsai, Yu Mei Shen, Pei Hsun Kao, I. Son Ng, Jo Shu Chang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

β-carotene is a valuable pigment abundant in some microalgal species but the low β-carotene productivity of microalgae has become the major obstacles against its commercialization. This work aims to improve the productivity of algae-based β-carotene via genetic engineering approaches. First, a synthetic psy gene construct (891 bp) encoding 297 amino acids is expressed in Scenedesmus sp. CPC2 host to enhance the β-carotene production. The synthetic psy gene is designed by considering the highest consensus of amino acids (i.e., 62% identity) from Chlamydomonas reinhardtii, Dunaliella salina, and Mariella zofingiensis. The original β-carotene content in wild-type Scenedesmus sp. CPC2 is 10.8 mg g−1-cell when grown on BG11 medium under 2% CO2 aeration, 150 μmol m−2 s−1 light intensity and 25°C. After transformation of the psy gene into the microalgal host, the β-carotene content of the best recombinant strain (i.e., transformant CPC2-4) significantly increased to over 30 mg g−1-cell. The optimal production of β-carotene with the CPC2-4 recombinant strain was achieved when the strain is grown on BG11 medium amended with 0.075 g of MgSO4, giving approximately 3-fold higher β-carotene content than that of the wild-type strain. The best cellular β-carotene content obtained (i.e., 31.8 mg g−1) is superior to most algae-based β-carotene production performance reported in the literature.

Original languageEnglish
Article number1700204
JournalBiotechnology Journal
Volume12
Issue number11
DOIs
Publication statusPublished - 2017 Nov

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Scenedesmus
Carotenoids
Genes
Synthetic Genes
phytoene
Microalgae
Amino Acids
Chlamydomonas reinhardtii
Genetic Engineering

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Molecular Medicine

Cite this

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title = "Expression of Synthetic Phytoene Synthase Gene to Enhance β-Carotene Production in Scenedesmus sp. CPC2",
abstract = "β-carotene is a valuable pigment abundant in some microalgal species but the low β-carotene productivity of microalgae has become the major obstacles against its commercialization. This work aims to improve the productivity of algae-based β-carotene via genetic engineering approaches. First, a synthetic psy gene construct (891 bp) encoding 297 amino acids is expressed in Scenedesmus sp. CPC2 host to enhance the β-carotene production. The synthetic psy gene is designed by considering the highest consensus of amino acids (i.e., 62{\%} identity) from Chlamydomonas reinhardtii, Dunaliella salina, and Mariella zofingiensis. The original β-carotene content in wild-type Scenedesmus sp. CPC2 is 10.8 mg g−1-cell when grown on BG11 medium under 2{\%} CO2 aeration, 150 μmol m−2 s−1 light intensity and 25°C. After transformation of the psy gene into the microalgal host, the β-carotene content of the best recombinant strain (i.e., transformant CPC2-4) significantly increased to over 30 mg g−1-cell. The optimal production of β-carotene with the CPC2-4 recombinant strain was achieved when the strain is grown on BG11 medium amended with 0.075 g of MgSO4, giving approximately 3-fold higher β-carotene content than that of the wild-type strain. The best cellular β-carotene content obtained (i.e., 31.8 mg g−1) is superior to most algae-based β-carotene production performance reported in the literature.",
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year = "2017",
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Expression of Synthetic Phytoene Synthase Gene to Enhance β-Carotene Production in Scenedesmus sp. CPC2. / Chen, Chun Yen; Kao, Ai Ling; Tsai, Zheng Chia; Shen, Yu Mei; Kao, Pei Hsun; Ng, I. Son; Chang, Jo Shu.

In: Biotechnology Journal, Vol. 12, No. 11, 1700204, 11.2017.

Research output: Contribution to journalArticle

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AU - Chen, Chun Yen

AU - Kao, Ai Ling

AU - Tsai, Zheng Chia

AU - Shen, Yu Mei

AU - Kao, Pei Hsun

AU - Ng, I. Son

AU - Chang, Jo Shu

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