Expression of the carbohydrate recognition domain of lung surfactant protein d and demonstration of its binding to lipopolysaccharides of gram-negative bacteria

Boon Leong Lim, Jiu-Yao Wang, Uffe Holmskov, Hans Jürgen Hoppe, Kenneth B.M. Reid

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Surfactant protein D is a collagenous C-type lectin (collectin) that is found almost exclusively in the lung. A recombinant protein, composed of the neck-region and the carbohydrate binding domain of bovine lung surfactant protein D, has been overexpressed in E. coli. The recombinant protein showed the same sugar binding specificity as the native protein and was able to bind to the lipopolysaccharides of several strains of Gram-negative bacteria, such as Klebsiella pneumoniae, Pseudomonas aeruginosa and Escherichia coli, which are known to cause lung infections. This binding was calcium-dependent and was inhibited by maltose. Native bovine surfactant protein D was also shown to be able to bind to these lipopolysaccharides in the same manner.

Original languageEnglish
Pages (from-to)1674-1680
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume202
Issue number3
DOIs
Publication statusPublished - 1994 Aug 15

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Pulmonary Surfactant-Associated Proteins
Pulmonary Surfactant-Associated Protein D
Gram-Negative Bacteria
Lipopolysaccharides
Bacteria
Demonstrations
Carbohydrates
Recombinant Proteins
Escherichia coli
C-Type Lectins
Maltose
Collectins
Sugars
Lung
Klebsiella pneumoniae
Pseudomonas aeruginosa
Calcium
Neck
Proteins
Infection

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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Expression of the carbohydrate recognition domain of lung surfactant protein d and demonstration of its binding to lipopolysaccharides of gram-negative bacteria. / Lim, Boon Leong; Wang, Jiu-Yao; Holmskov, Uffe; Hoppe, Hans Jürgen; Reid, Kenneth B.M.

In: Biochemical and Biophysical Research Communications, Vol. 202, No. 3, 15.08.1994, p. 1674-1680.

Research output: Contribution to journalArticle

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