Expression of various CDC25B isoforms in human spermatozoa

Yen Ni Teng; Chia Ling Chung; Yung Ming Lin; Hsien An Pan; Rui Wen Liao; Pao Lin Kuo

doi:10.1016/j.fertnstert.2006.11.186

Expression of various CDC25B isoforms in human spermatozoa

Yen Ni Teng, Chia Ling Chung, Yung Ming Lin, Hsien An Pan, Rui Wen Liao, Pao Lin Kuo

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Objective: To explore the role of CDC25B protein in postmeiotic germ cells. Design: In vitro experiment. Setting: University-based reproductive genetics laboratory. Patient(s): Fertile and infertile volunteers. Intervention(s): Reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR, and immunostaining for CDC25B. Main Outcome Measure: Expression profiling of CDC25B in human spermatozoa. Result(s): Four splicing variants (CDC25B1, B2, B3, and B4) are expressed in human spermatozoa. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa, and weak staining in the principal piece and cytosol of the head. The messenger RNA (mRNA) transcript level of CDC25B was increased in sperm samples of men with asthenospermia. Conclusion(s): The expression of CDC25B in different cellular compartments of human spermatozoa suggests that there are diverse noncell-cycle-related functions of CDC25B in terminally differentiated human germ cells.

Original language	English
Pages (from-to)	379-382
Number of pages	4
Journal	Fertility and Sterility
Volume	88
Issue number	2
DOIs	https://doi.org/10.1016/j.fertnstert.2006.11.186
Publication status	Published - 2007 Aug

All Science Journal Classification (ASJC) codes

Reproductive Medicine
Obstetrics and Gynaecology

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10.1016/j.fertnstert.2006.11.186

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@article{6798cc8144c14dfab11b27f00bcc4349,

title = "Expression of various CDC25B isoforms in human spermatozoa",

abstract = "Objective: To explore the role of CDC25B protein in postmeiotic germ cells. Design: In vitro experiment. Setting: University-based reproductive genetics laboratory. Patient(s): Fertile and infertile volunteers. Intervention(s): Reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR, and immunostaining for CDC25B. Main Outcome Measure: Expression profiling of CDC25B in human spermatozoa. Result(s): Four splicing variants (CDC25B1, B2, B3, and B4) are expressed in human spermatozoa. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa, and weak staining in the principal piece and cytosol of the head. The messenger RNA (mRNA) transcript level of CDC25B was increased in sperm samples of men with asthenospermia. Conclusion(s): The expression of CDC25B in different cellular compartments of human spermatozoa suggests that there are diverse noncell-cycle-related functions of CDC25B in terminally differentiated human germ cells.",

author = "Teng, {Yen Ni} and Chung, {Chia Ling} and Lin, {Yung Ming} and Pan, {Hsien An} and Liao, {Rui Wen} and Kuo, {Pao Lin}",

note = "Funding Information: Supported by grants NSC 94-2314-B-006-075, NSC 94-2314-B-006-076, and NSC 94-2320-B-041-012 from the National Science Council of Taiwan, Taipei, Taiwan. ",

year = "2007",

month = aug,

doi = "10.1016/j.fertnstert.2006.11.186",

language = "English",

volume = "88",

pages = "379--382",

journal = "Fertility and Sterility",

issn = "0015-0282",

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number = "2",

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TY - JOUR

T1 - Expression of various CDC25B isoforms in human spermatozoa

AU - Teng, Yen Ni

AU - Chung, Chia Ling

AU - Lin, Yung Ming

AU - Pan, Hsien An

AU - Liao, Rui Wen

AU - Kuo, Pao Lin

N1 - Funding Information: Supported by grants NSC 94-2314-B-006-075, NSC 94-2314-B-006-076, and NSC 94-2320-B-041-012 from the National Science Council of Taiwan, Taipei, Taiwan.

PY - 2007/8

Y1 - 2007/8

N2 - Objective: To explore the role of CDC25B protein in postmeiotic germ cells. Design: In vitro experiment. Setting: University-based reproductive genetics laboratory. Patient(s): Fertile and infertile volunteers. Intervention(s): Reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR, and immunostaining for CDC25B. Main Outcome Measure: Expression profiling of CDC25B in human spermatozoa. Result(s): Four splicing variants (CDC25B1, B2, B3, and B4) are expressed in human spermatozoa. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa, and weak staining in the principal piece and cytosol of the head. The messenger RNA (mRNA) transcript level of CDC25B was increased in sperm samples of men with asthenospermia. Conclusion(s): The expression of CDC25B in different cellular compartments of human spermatozoa suggests that there are diverse noncell-cycle-related functions of CDC25B in terminally differentiated human germ cells.

AB - Objective: To explore the role of CDC25B protein in postmeiotic germ cells. Design: In vitro experiment. Setting: University-based reproductive genetics laboratory. Patient(s): Fertile and infertile volunteers. Intervention(s): Reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR, and immunostaining for CDC25B. Main Outcome Measure: Expression profiling of CDC25B in human spermatozoa. Result(s): Four splicing variants (CDC25B1, B2, B3, and B4) are expressed in human spermatozoa. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa, and weak staining in the principal piece and cytosol of the head. The messenger RNA (mRNA) transcript level of CDC25B was increased in sperm samples of men with asthenospermia. Conclusion(s): The expression of CDC25B in different cellular compartments of human spermatozoa suggests that there are diverse noncell-cycle-related functions of CDC25B in terminally differentiated human germ cells.

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Expression of various CDC25B isoforms in human spermatozoa

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