Abstract
Objective: To explore the role of CDC25B protein in postmeiotic germ cells. Design: In vitro experiment. Setting: University-based reproductive genetics laboratory. Patient(s): Fertile and infertile volunteers. Intervention(s): Reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR, and immunostaining for CDC25B. Main Outcome Measure: Expression profiling of CDC25B in human spermatozoa. Result(s): Four splicing variants (CDC25B1, B2, B3, and B4) are expressed in human spermatozoa. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa, and weak staining in the principal piece and cytosol of the head. The messenger RNA (mRNA) transcript level of CDC25B was increased in sperm samples of men with asthenospermia. Conclusion(s): The expression of CDC25B in different cellular compartments of human spermatozoa suggests that there are diverse noncell-cycle-related functions of CDC25B in terminally differentiated human germ cells.
| Original language | English |
|---|---|
| Pages (from-to) | 379-382 |
| Number of pages | 4 |
| Journal | Fertility and Sterility |
| Volume | 88 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2007 Aug 1 |
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All Science Journal Classification (ASJC) codes
- Reproductive Medicine
- Obstetrics and Gynaecology
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Expression of various CDC25B isoforms in human spermatozoa. / Teng, Yen Ni; Chung, Chia Ling; Lin, YungMing; Pan, Hsien An; Liao, Rui Wen; Kuo, Pao-Lin.
In: Fertility and Sterility, Vol. 88, No. 2, 01.08.2007, p. 379-382.Research output: Contribution to journal › Article
TY - JOUR
T1 - Expression of various CDC25B isoforms in human spermatozoa
AU - Teng, Yen Ni
AU - Chung, Chia Ling
AU - Lin, YungMing
AU - Pan, Hsien An
AU - Liao, Rui Wen
AU - Kuo, Pao-Lin
PY - 2007/8/1
Y1 - 2007/8/1
N2 - Objective: To explore the role of CDC25B protein in postmeiotic germ cells. Design: In vitro experiment. Setting: University-based reproductive genetics laboratory. Patient(s): Fertile and infertile volunteers. Intervention(s): Reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR, and immunostaining for CDC25B. Main Outcome Measure: Expression profiling of CDC25B in human spermatozoa. Result(s): Four splicing variants (CDC25B1, B2, B3, and B4) are expressed in human spermatozoa. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa, and weak staining in the principal piece and cytosol of the head. The messenger RNA (mRNA) transcript level of CDC25B was increased in sperm samples of men with asthenospermia. Conclusion(s): The expression of CDC25B in different cellular compartments of human spermatozoa suggests that there are diverse noncell-cycle-related functions of CDC25B in terminally differentiated human germ cells.
AB - Objective: To explore the role of CDC25B protein in postmeiotic germ cells. Design: In vitro experiment. Setting: University-based reproductive genetics laboratory. Patient(s): Fertile and infertile volunteers. Intervention(s): Reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR, and immunostaining for CDC25B. Main Outcome Measure: Expression profiling of CDC25B in human spermatozoa. Result(s): Four splicing variants (CDC25B1, B2, B3, and B4) are expressed in human spermatozoa. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa, and weak staining in the principal piece and cytosol of the head. The messenger RNA (mRNA) transcript level of CDC25B was increased in sperm samples of men with asthenospermia. Conclusion(s): The expression of CDC25B in different cellular compartments of human spermatozoa suggests that there are diverse noncell-cycle-related functions of CDC25B in terminally differentiated human germ cells.
UR - http://www.scopus.com/inward/record.url?scp=34547677886&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34547677886&partnerID=8YFLogxK
U2 - 10.1016/j.fertnstert.2006.11.186
DO - 10.1016/j.fertnstert.2006.11.186
M3 - Article
C2 - 17336969
AN - SCOPUS:34547677886
VL - 88
SP - 379
EP - 382
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 2
ER -