Expression of various CDC25B isoforms in human spermatozoa

Yen Ni Teng, Chia Ling Chung, YungMing Lin, Hsien An Pan, Rui Wen Liao, Pao-Lin Kuo

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective: To explore the role of CDC25B protein in postmeiotic germ cells. Design: In vitro experiment. Setting: University-based reproductive genetics laboratory. Patient(s): Fertile and infertile volunteers. Intervention(s): Reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR, and immunostaining for CDC25B. Main Outcome Measure: Expression profiling of CDC25B in human spermatozoa. Result(s): Four splicing variants (CDC25B1, B2, B3, and B4) are expressed in human spermatozoa. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa, and weak staining in the principal piece and cytosol of the head. The messenger RNA (mRNA) transcript level of CDC25B was increased in sperm samples of men with asthenospermia. Conclusion(s): The expression of CDC25B in different cellular compartments of human spermatozoa suggests that there are diverse noncell-cycle-related functions of CDC25B in terminally differentiated human germ cells.

Original languageEnglish
Pages (from-to)379-382
Number of pages4
JournalFertility and Sterility
Volume88
Issue number2
DOIs
Publication statusPublished - 2007 Aug 1

Fingerprint

Spermatozoa
Protein Isoforms
Staining and Labeling
Germ Cells
Reverse Transcription
Cytosol
Fluorescent Antibody Technique
Real-Time Polymerase Chain Reaction
Volunteers
Head
Outcome Assessment (Health Care)
Polymerase Chain Reaction
Messenger RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

Teng, Yen Ni ; Chung, Chia Ling ; Lin, YungMing ; Pan, Hsien An ; Liao, Rui Wen ; Kuo, Pao-Lin. / Expression of various CDC25B isoforms in human spermatozoa. In: Fertility and Sterility. 2007 ; Vol. 88, No. 2. pp. 379-382.
@article{6798cc8144c14dfab11b27f00bcc4349,
title = "Expression of various CDC25B isoforms in human spermatozoa",
abstract = "Objective: To explore the role of CDC25B protein in postmeiotic germ cells. Design: In vitro experiment. Setting: University-based reproductive genetics laboratory. Patient(s): Fertile and infertile volunteers. Intervention(s): Reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR, and immunostaining for CDC25B. Main Outcome Measure: Expression profiling of CDC25B in human spermatozoa. Result(s): Four splicing variants (CDC25B1, B2, B3, and B4) are expressed in human spermatozoa. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa, and weak staining in the principal piece and cytosol of the head. The messenger RNA (mRNA) transcript level of CDC25B was increased in sperm samples of men with asthenospermia. Conclusion(s): The expression of CDC25B in different cellular compartments of human spermatozoa suggests that there are diverse noncell-cycle-related functions of CDC25B in terminally differentiated human germ cells.",
author = "Teng, {Yen Ni} and Chung, {Chia Ling} and YungMing Lin and Pan, {Hsien An} and Liao, {Rui Wen} and Pao-Lin Kuo",
year = "2007",
month = "8",
day = "1",
doi = "10.1016/j.fertnstert.2006.11.186",
language = "English",
volume = "88",
pages = "379--382",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "2",

}

Expression of various CDC25B isoforms in human spermatozoa. / Teng, Yen Ni; Chung, Chia Ling; Lin, YungMing; Pan, Hsien An; Liao, Rui Wen; Kuo, Pao-Lin.

In: Fertility and Sterility, Vol. 88, No. 2, 01.08.2007, p. 379-382.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of various CDC25B isoforms in human spermatozoa

AU - Teng, Yen Ni

AU - Chung, Chia Ling

AU - Lin, YungMing

AU - Pan, Hsien An

AU - Liao, Rui Wen

AU - Kuo, Pao-Lin

PY - 2007/8/1

Y1 - 2007/8/1

N2 - Objective: To explore the role of CDC25B protein in postmeiotic germ cells. Design: In vitro experiment. Setting: University-based reproductive genetics laboratory. Patient(s): Fertile and infertile volunteers. Intervention(s): Reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR, and immunostaining for CDC25B. Main Outcome Measure: Expression profiling of CDC25B in human spermatozoa. Result(s): Four splicing variants (CDC25B1, B2, B3, and B4) are expressed in human spermatozoa. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa, and weak staining in the principal piece and cytosol of the head. The messenger RNA (mRNA) transcript level of CDC25B was increased in sperm samples of men with asthenospermia. Conclusion(s): The expression of CDC25B in different cellular compartments of human spermatozoa suggests that there are diverse noncell-cycle-related functions of CDC25B in terminally differentiated human germ cells.

AB - Objective: To explore the role of CDC25B protein in postmeiotic germ cells. Design: In vitro experiment. Setting: University-based reproductive genetics laboratory. Patient(s): Fertile and infertile volunteers. Intervention(s): Reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR, and immunostaining for CDC25B. Main Outcome Measure: Expression profiling of CDC25B in human spermatozoa. Result(s): Four splicing variants (CDC25B1, B2, B3, and B4) are expressed in human spermatozoa. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa, and weak staining in the principal piece and cytosol of the head. The messenger RNA (mRNA) transcript level of CDC25B was increased in sperm samples of men with asthenospermia. Conclusion(s): The expression of CDC25B in different cellular compartments of human spermatozoa suggests that there are diverse noncell-cycle-related functions of CDC25B in terminally differentiated human germ cells.

UR - http://www.scopus.com/inward/record.url?scp=34547677886&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547677886&partnerID=8YFLogxK

U2 - 10.1016/j.fertnstert.2006.11.186

DO - 10.1016/j.fertnstert.2006.11.186

M3 - Article

C2 - 17336969

AN - SCOPUS:34547677886

VL - 88

SP - 379

EP - 382

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 2

ER -