Expression of various CDC25B isoforms in human spermatozoa

Yen Ni Teng, Chia Ling Chung, YungMing Lin, Hsien An Pan, Rui Wen Liao, Pao-Lin Kuo

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Objective: To explore the role of CDC25B protein in postmeiotic germ cells. Design: In vitro experiment. Setting: University-based reproductive genetics laboratory. Patient(s): Fertile and infertile volunteers. Intervention(s): Reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR, and immunostaining for CDC25B. Main Outcome Measure: Expression profiling of CDC25B in human spermatozoa. Result(s): Four splicing variants (CDC25B1, B2, B3, and B4) are expressed in human spermatozoa. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa, and weak staining in the principal piece and cytosol of the head. The messenger RNA (mRNA) transcript level of CDC25B was increased in sperm samples of men with asthenospermia. Conclusion(s): The expression of CDC25B in different cellular compartments of human spermatozoa suggests that there are diverse noncell-cycle-related functions of CDC25B in terminally differentiated human germ cells.

Original languageEnglish
Pages (from-to)379-382
Number of pages4
JournalFertility and Sterility
Volume88
Issue number2
DOIs
Publication statusPublished - 2007 Aug 1

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology

Fingerprint Dive into the research topics of 'Expression of various CDC25B isoforms in human spermatozoa'. Together they form a unique fingerprint.

Cite this