Expression of WW domain–Containing oxidoreductase WWOX in pterygium

Yi-Hsun Huang, Nan-Shan Chang, Sung Huei Tseng

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Purpose: Pterygium was traditionally regarded as a degenerative disease, but certain characteristics suggest that pterygium is probably premalignant tissue. The human WWOX gene, encoding the WW domain containing oxidoreductase (WWOX, FOR, or WOX1), is a candidate tumor suppressor gene. In this study, we investigated the WWOX gene and protein expression in pterygium. Methods: Pterygium tissues were obtained from patients (n=16, primary=8, recurrent=8) who received surgical excisions. Each tissue sample was further divided into head and body regions. The WWOX gene and protein expression were examined with immunohistochemistry, western blot, and quantitative PCR. For comparison, normal superior temporal bulbar conjunctivas were used as controls. Results: Compared to the controls, upregulation of WWOX and its Tyr33 phosphorylation was observed in the head region of all pterygium specimens. In the head and body of the pterygium specimens, WWOX expression was significantly higher than in the controls. In addition, WWOX expression was stronger in recurrent pterygia than in primary pterygia. Conclusions: Increased WWOX expression, especially in the head region, is probably due to the invasiveness of the pterygium. Our results indicate that WWOX may play a role in pterygium progression and recurrence.

Original languageEnglish
Pages (from-to)711-717
Number of pages7
JournalMolecular Vision
Volume21
Publication statusPublished - 2015 Jun 25

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Pterygium
Oxidoreductases
Head
Gene Expression
Body Regions
Conjunctiva
Tumor Suppressor Genes
Proteins
Up-Regulation
Western Blotting
Immunohistochemistry
Phosphorylation

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

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abstract = "Purpose: Pterygium was traditionally regarded as a degenerative disease, but certain characteristics suggest that pterygium is probably premalignant tissue. The human WWOX gene, encoding the WW domain containing oxidoreductase (WWOX, FOR, or WOX1), is a candidate tumor suppressor gene. In this study, we investigated the WWOX gene and protein expression in pterygium. Methods: Pterygium tissues were obtained from patients (n=16, primary=8, recurrent=8) who received surgical excisions. Each tissue sample was further divided into head and body regions. The WWOX gene and protein expression were examined with immunohistochemistry, western blot, and quantitative PCR. For comparison, normal superior temporal bulbar conjunctivas were used as controls. Results: Compared to the controls, upregulation of WWOX and its Tyr33 phosphorylation was observed in the head region of all pterygium specimens. In the head and body of the pterygium specimens, WWOX expression was significantly higher than in the controls. In addition, WWOX expression was stronger in recurrent pterygia than in primary pterygia. Conclusions: Increased WWOX expression, especially in the head region, is probably due to the invasiveness of the pterygium. Our results indicate that WWOX may play a role in pterygium progression and recurrence.",
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Expression of WW domain–Containing oxidoreductase WWOX in pterygium. / Huang, Yi-Hsun; Chang, Nan-Shan; Tseng, Sung Huei.

In: Molecular Vision, Vol. 21, 25.06.2015, p. 711-717.

Research output: Contribution to journalArticle

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