Expression patterns and transcript concentrations of the autosomal DAZL gene in testes of azoospermic men

Yung Ming Lin, Chaio Wei Chen, H. Sunny Sun, Shaw Jeng Tsai, Chao Chin Hsu, Yen Ni Teng, Johnny Shinn Nan Lin, Pao Lin Kuo

Research output: Contribution to journalArticle

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Abstract

The DAZ (Deleted in AZoospermia) gene cluster on the Y chromosome is a strong candidate for the azoospermia factor. The DAZ gene was derived from an autosomal homologue, DAZL (DAZ-Like). This study was designed to assess the functional role of DAZL in human spermatogenesis. The expression patterns and mRNA transcript levels of DAZL in the testes of 17 azoospermic men were therefore examined by immunohistochemical staining and quantitative competitive reverse transcription-polymerase chain reaction. DAZL protein was expressed in the cytoplasm of primary spermatocytes and weakly in spermatogonia. It was detected in the testicular tissues of all subjects with germ cells present. The copy number of the DAZL transcript in normal spermatogenesis (n = 4), hypospermatogenesis or maturation arrest (n = 6), and Sertoli cell-only syndrome (n = 7) ranged from 1.22 × 106 to 1.63 × 106 per ng of RNA, 1.19 × 105 to 2.82 × 105 per ng of RNA and 2.83 × 104 to 1.23 × 105 per ng of RNA respectively. DAZL transcripts were lower in men with spermatogenic failure, and a significant difference was found between the three groups (P < 0.0001). This study suggests that DAZL may play an important role in the human spermatogenic processes of both mitosis and meiosis.

Original languageEnglish
Pages (from-to)1015-1022
Number of pages8
JournalMolecular Human Reproduction
Volume7
Issue number11
Publication statusPublished - 2001 Nov 20

Fingerprint

Azoospermia
Testis
RNA
Spermatogenesis
Genes
Sertoli Cell-Only Syndrome
Oligospermia
Spermatogonia
Spermatocytes
Y Chromosome
Meiosis
Multigene Family
Mitosis
Germ Cells
Reverse Transcription
Cytoplasm
Staining and Labeling
Polymerase Chain Reaction
Messenger RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Embryology
  • Molecular Biology
  • Genetics
  • Obstetrics and Gynaecology
  • Developmental Biology
  • Cell Biology

Cite this

Lin, Yung Ming ; Chen, Chaio Wei ; Sun, H. Sunny ; Tsai, Shaw Jeng ; Hsu, Chao Chin ; Teng, Yen Ni ; Lin, Johnny Shinn Nan ; Kuo, Pao Lin. / Expression patterns and transcript concentrations of the autosomal DAZL gene in testes of azoospermic men. In: Molecular Human Reproduction. 2001 ; Vol. 7, No. 11. pp. 1015-1022.
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abstract = "The DAZ (Deleted in AZoospermia) gene cluster on the Y chromosome is a strong candidate for the azoospermia factor. The DAZ gene was derived from an autosomal homologue, DAZL (DAZ-Like). This study was designed to assess the functional role of DAZL in human spermatogenesis. The expression patterns and mRNA transcript levels of DAZL in the testes of 17 azoospermic men were therefore examined by immunohistochemical staining and quantitative competitive reverse transcription-polymerase chain reaction. DAZL protein was expressed in the cytoplasm of primary spermatocytes and weakly in spermatogonia. It was detected in the testicular tissues of all subjects with germ cells present. The copy number of the DAZL transcript in normal spermatogenesis (n = 4), hypospermatogenesis or maturation arrest (n = 6), and Sertoli cell-only syndrome (n = 7) ranged from 1.22 × 106 to 1.63 × 106 per ng of RNA, 1.19 × 105 to 2.82 × 105 per ng of RNA and 2.83 × 104 to 1.23 × 105 per ng of RNA respectively. DAZL transcripts were lower in men with spermatogenic failure, and a significant difference was found between the three groups (P < 0.0001). This study suggests that DAZL may play an important role in the human spermatogenic processes of both mitosis and meiosis.",
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Expression patterns and transcript concentrations of the autosomal DAZL gene in testes of azoospermic men. / Lin, Yung Ming; Chen, Chaio Wei; Sun, H. Sunny; Tsai, Shaw Jeng; Hsu, Chao Chin; Teng, Yen Ni; Lin, Johnny Shinn Nan; Kuo, Pao Lin.

In: Molecular Human Reproduction, Vol. 7, No. 11, 20.11.2001, p. 1015-1022.

Research output: Contribution to journalArticle

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