Extractive disruption process integration using ultrasonication and an aqueous two-phase system for protein recovery from Chlorella sorokiniana

Win Nee Phong, Cheng Foh Le, Pau Loke Show, Jo Shu Chang, Tau Chuan Ling

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Microalgae emerge as the most promising protein sources for aquaculture industry. However, the commercial proteins production at low cost remains a challenge. The process of harnessing microalgal proteins involves several steps such as cell disruption, isolation and extraction. The discrete processes are generally complicated, time-consuming and costly. To date, the notion of integrating microalgal cell disruption and proteins recovery process into one step is yet to explore. Hence, this study aimed to investigate the feasibility of applying methanol/potassium ATPS in the integrated process for proteins recovery from Chlorella sorokiniana. Parameters such as salt types, salt concentrations, methanol concentrations, NaCl addition were optimized. The possibility of upscaling and the effectiveness of recycling the phase components were also studied. The results showed that ATPS formed by 30% (w/w) K3PO4 and 20% (w/w) methanol with 3% (w/w) NaCl addition was optimum for proteins recovery. In this system, the partition coefficient and yield were 7.28 and 84.23%, respectively. There were no significant differences in the partition coefficient and yield when the integrated process was upscaled to 100-fold. The recovered phase components can still be recycled effectively at fifth cycle. In conclusions, this method is simple, rapid, environmental friendly and could be implemented at large scale.

Original languageEnglish
Pages (from-to)357-369
Number of pages13
JournalEngineering in Life Sciences
Volume17
Issue number4
DOIs
Publication statusPublished - 2017 Apr 1

Fingerprint

Chlorella
Proteins
Recovery
Methanol
Salts
Microalgae
Aquaculture
Cell Separation
Recycling
Potassium
Industry
Costs and Cost Analysis
Costs

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Environmental Engineering
  • Bioengineering

Cite this

@article{38fa552be7df4e7db82fe33664ed5c01,
title = "Extractive disruption process integration using ultrasonication and an aqueous two-phase system for protein recovery from Chlorella sorokiniana",
abstract = "Microalgae emerge as the most promising protein sources for aquaculture industry. However, the commercial proteins production at low cost remains a challenge. The process of harnessing microalgal proteins involves several steps such as cell disruption, isolation and extraction. The discrete processes are generally complicated, time-consuming and costly. To date, the notion of integrating microalgal cell disruption and proteins recovery process into one step is yet to explore. Hence, this study aimed to investigate the feasibility of applying methanol/potassium ATPS in the integrated process for proteins recovery from Chlorella sorokiniana. Parameters such as salt types, salt concentrations, methanol concentrations, NaCl addition were optimized. The possibility of upscaling and the effectiveness of recycling the phase components were also studied. The results showed that ATPS formed by 30{\%} (w/w) K3PO4 and 20{\%} (w/w) methanol with 3{\%} (w/w) NaCl addition was optimum for proteins recovery. In this system, the partition coefficient and yield were 7.28 and 84.23{\%}, respectively. There were no significant differences in the partition coefficient and yield when the integrated process was upscaled to 100-fold. The recovered phase components can still be recycled effectively at fifth cycle. In conclusions, this method is simple, rapid, environmental friendly and could be implemented at large scale.",
author = "Phong, {Win Nee} and Le, {Cheng Foh} and Show, {Pau Loke} and Chang, {Jo Shu} and Ling, {Tau Chuan}",
year = "2017",
month = "4",
day = "1",
doi = "10.1002/elsc.201600133",
language = "English",
volume = "17",
pages = "357--369",
journal = "Engineering in Life Sciences",
issn = "1618-0240",
publisher = "Wiley-VCH Verlag",
number = "4",

}

Extractive disruption process integration using ultrasonication and an aqueous two-phase system for protein recovery from Chlorella sorokiniana. / Phong, Win Nee; Le, Cheng Foh; Show, Pau Loke; Chang, Jo Shu; Ling, Tau Chuan.

In: Engineering in Life Sciences, Vol. 17, No. 4, 01.04.2017, p. 357-369.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Extractive disruption process integration using ultrasonication and an aqueous two-phase system for protein recovery from Chlorella sorokiniana

AU - Phong, Win Nee

AU - Le, Cheng Foh

AU - Show, Pau Loke

AU - Chang, Jo Shu

AU - Ling, Tau Chuan

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Microalgae emerge as the most promising protein sources for aquaculture industry. However, the commercial proteins production at low cost remains a challenge. The process of harnessing microalgal proteins involves several steps such as cell disruption, isolation and extraction. The discrete processes are generally complicated, time-consuming and costly. To date, the notion of integrating microalgal cell disruption and proteins recovery process into one step is yet to explore. Hence, this study aimed to investigate the feasibility of applying methanol/potassium ATPS in the integrated process for proteins recovery from Chlorella sorokiniana. Parameters such as salt types, salt concentrations, methanol concentrations, NaCl addition were optimized. The possibility of upscaling and the effectiveness of recycling the phase components were also studied. The results showed that ATPS formed by 30% (w/w) K3PO4 and 20% (w/w) methanol with 3% (w/w) NaCl addition was optimum for proteins recovery. In this system, the partition coefficient and yield were 7.28 and 84.23%, respectively. There were no significant differences in the partition coefficient and yield when the integrated process was upscaled to 100-fold. The recovered phase components can still be recycled effectively at fifth cycle. In conclusions, this method is simple, rapid, environmental friendly and could be implemented at large scale.

AB - Microalgae emerge as the most promising protein sources for aquaculture industry. However, the commercial proteins production at low cost remains a challenge. The process of harnessing microalgal proteins involves several steps such as cell disruption, isolation and extraction. The discrete processes are generally complicated, time-consuming and costly. To date, the notion of integrating microalgal cell disruption and proteins recovery process into one step is yet to explore. Hence, this study aimed to investigate the feasibility of applying methanol/potassium ATPS in the integrated process for proteins recovery from Chlorella sorokiniana. Parameters such as salt types, salt concentrations, methanol concentrations, NaCl addition were optimized. The possibility of upscaling and the effectiveness of recycling the phase components were also studied. The results showed that ATPS formed by 30% (w/w) K3PO4 and 20% (w/w) methanol with 3% (w/w) NaCl addition was optimum for proteins recovery. In this system, the partition coefficient and yield were 7.28 and 84.23%, respectively. There were no significant differences in the partition coefficient and yield when the integrated process was upscaled to 100-fold. The recovered phase components can still be recycled effectively at fifth cycle. In conclusions, this method is simple, rapid, environmental friendly and could be implemented at large scale.

UR - http://www.scopus.com/inward/record.url?scp=85006410850&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85006410850&partnerID=8YFLogxK

U2 - 10.1002/elsc.201600133

DO - 10.1002/elsc.201600133

M3 - Article

AN - SCOPUS:85006410850

VL - 17

SP - 357

EP - 369

JO - Engineering in Life Sciences

JF - Engineering in Life Sciences

SN - 1618-0240

IS - 4

ER -