TY - GEN
T1 - Fabrication of cellulose-gelatin based endothelialed vascular graft with SMCs/ADSCs seeding in bioreactor
AU - Yu, Ts Ching
AU - Yeh, Ming Long
N1 - Publisher Copyright:
© Springer International Publishing Switzerland 2015.
PY - 2015
Y1 - 2015
N2 - Cardiovascular disease (CVD) is a major health issue over the worldwide. The application of tissue engineering technology holds great promise for improving outcomes in CVD patients. Currently, the use of synthetic vascular grafts has several limitations, including thrombogenicity, increased risk of infection, and lack of growth potential. Natural vascular grafts have many advantages including excellent degradation, biocompatibility, but low mechanical property. Therefore, to form a biodegradable and antithrombotic vessel graft as temporary substitute is a problem to overcome in recent researches. The purpose of our study is using natural materials to rapidly form an endothelialized vascular graft in vitro. We utilized cellulose and gelatin to fabricate a composite scaffold. In order to enhance the mechanical property and decrease the degradation rate of scaffold, we added genipin to crosslink cellulose and gelatin. We mixed the cellulose, gelatin, and genipin solution, and injected solution into self-made mold to form tubular hydrogel. At last, we could obtain the tubular scaffold by freeze-drying. With regard to fabrication of vascular graft, the smooth muscle cells (SMCs) extracted from rabbit’s carotid arteries would be seeded into scaffold to secret the collagen and elastic fiber, while adipose derived stem cell (AD- SCs) would be seeded onto the lumen of scaffold as the source of endothelial cells. The material property of scaffold were analyzed by swelling test, biocompability, and FTIR. Moreover, we observe the SMCs distribution and extracellular matrix (ECM) deposition of vascular graft by histology.
AB - Cardiovascular disease (CVD) is a major health issue over the worldwide. The application of tissue engineering technology holds great promise for improving outcomes in CVD patients. Currently, the use of synthetic vascular grafts has several limitations, including thrombogenicity, increased risk of infection, and lack of growth potential. Natural vascular grafts have many advantages including excellent degradation, biocompatibility, but low mechanical property. Therefore, to form a biodegradable and antithrombotic vessel graft as temporary substitute is a problem to overcome in recent researches. The purpose of our study is using natural materials to rapidly form an endothelialized vascular graft in vitro. We utilized cellulose and gelatin to fabricate a composite scaffold. In order to enhance the mechanical property and decrease the degradation rate of scaffold, we added genipin to crosslink cellulose and gelatin. We mixed the cellulose, gelatin, and genipin solution, and injected solution into self-made mold to form tubular hydrogel. At last, we could obtain the tubular scaffold by freeze-drying. With regard to fabrication of vascular graft, the smooth muscle cells (SMCs) extracted from rabbit’s carotid arteries would be seeded into scaffold to secret the collagen and elastic fiber, while adipose derived stem cell (AD- SCs) would be seeded onto the lumen of scaffold as the source of endothelial cells. The material property of scaffold were analyzed by swelling test, biocompability, and FTIR. Moreover, we observe the SMCs distribution and extracellular matrix (ECM) deposition of vascular graft by histology.
UR - http://www.scopus.com/inward/record.url?scp=84947419672&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84947419672&partnerID=8YFLogxK
U2 - 10.1007/978-3-319-19452-3_10
DO - 10.1007/978-3-319-19452-3_10
M3 - Conference contribution
AN - SCOPUS:84947419672
SN - 9783319194516
T3 - IFMBE Proceedings
SP - 35
EP - 38
BT - 7th WACBE World Congress on Bioengineering, 2015
A2 - Lim, Chwee Teck
A2 - Goh, James
PB - Springer Verlag
T2 - 7th World Congress on Bioengineering, WACBE 2015
Y2 - 6 July 2015 through 8 July 2015
ER -