Fabrication of cellulose-gelatin based endothelialed vascular graft with SMCs/ADSCs seeding in bioreactor

Ts Ching Yu, Ming-Long Yeh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Cardiovascular disease (CVD) is a major health issue over the worldwide. The application of tissue engineering technology holds great promise for improving outcomes in CVD patients. Currently, the use of synthetic vascular grafts has several limitations, including thrombogenicity, increased risk of infection, and lack of growth potential. Natural vascular grafts have many advantages including excellent degradation, biocompatibility, but low mechanical property. Therefore, to form a biodegradable and antithrombotic vessel graft as temporary substitute is a problem to overcome in recent researches. The purpose of our study is using natural materials to rapidly form an endothelialized vascular graft in vitro. We utilized cellulose and gelatin to fabricate a composite scaffold. In order to enhance the mechanical property and decrease the degradation rate of scaffold, we added genipin to crosslink cellulose and gelatin. We mixed the cellulose, gelatin, and genipin solution, and injected solution into self-made mold to form tubular hydrogel. At last, we could obtain the tubular scaffold by freeze-drying. With regard to fabrication of vascular graft, the smooth muscle cells (SMCs) extracted from rabbit’s carotid arteries would be seeded into scaffold to secret the collagen and elastic fiber, while adipose derived stem cell (AD- SCs) would be seeded onto the lumen of scaffold as the source of endothelial cells. The material property of scaffold were analyzed by swelling test, biocompability, and FTIR. Moreover, we observe the SMCs distribution and extracellular matrix (ECM) deposition of vascular graft by histology.

Original languageEnglish
Title of host publication7th WACBE World Congress on Bioengineering, 2015
EditorsChwee Teck Lim, James Goh
PublisherSpringer Verlag
Pages35-38
Number of pages4
ISBN (Print)9783319194516
DOIs
Publication statusPublished - 2015 Jan 1
Event7th World Congress on Bioengineering, WACBE 2015 - Kent Ridge, Singapore
Duration: 2015 Jul 62015 Jul 8

Publication series

NameIFMBE Proceedings
Volume52
ISSN (Print)1680-0737

Other

Other7th World Congress on Bioengineering, WACBE 2015
CountrySingapore
CityKent Ridge
Period15-07-0615-07-08

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomedical Engineering

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