Fabrication of long poly(dimethyl siloxane) nanochannels by replicating protein deposit from confined solution evaporation

Kuo Feng Lo, Yi-Je Juang

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

A relatively simple, inexpensive and reliable technique was developed to fabricate an array of nanochannels. Moreover, the nanochannels are directly integrated to microchannels as a whole, which facilitates solution loading from the millimeter-scaled loading reservoirs into the nanochannels. It is found that continuous bovine serum albumin (BSA) line structures with triangle-like cross section at nanoscale can be obtained by evaporation of BSA solution with concentration between 0.5 wt. % and 1 wt. % inside the microchannels. The poly(dimethyl siloxane) nanochannels were replicated from these line structures, followed by sealing with the glass slide. The DNA molecules can be stretched inside the nanochannels as fabricated.

Original languageEnglish
Article number026504
JournalBiomicrofluidics
Volume6
Issue number2
DOIs
Publication statusPublished - 2012 Apr 26

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Materials Science(all)
  • Genetics
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

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