TY - JOUR
T1 - Facile preparation of electroactive graphene derivative and its potential application in electrochemical detection
AU - Chen, Hsiao-Chien
AU - Yang, Hung Wei
AU - Yang, Kuang Hsuan
AU - Chen, Ching Hsiang
AU - Hou, Chung Che
AU - Tu, Yi Ming
N1 - Funding Information:
The authors thank the Vanung University and Ministry of Science and Technology (MOST) of ROC (MOST 104-2221-E-238-007 and MOST 104-2622-E-238-004-CC3) for their financial support
Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2017/3/1
Y1 - 2017/3/1
N2 - A one-step process combining chemical modification and exfoliation for obtaining a graphene (Gs) derivative from pristine graphite is conducted through Friedel–Crafts chemical acylation. This electrophilic substitution reaction results in the carboxylic group (–COOH) of niacin transforming into an acylium ion (–C+[dbnd]O), which is an active species that replaces the sp2 C[sbnd]H of graphite. In addition, highly viscous polyphosphoric acid under mechanical stirring was used to exert strong shear stress on and consequently exfoliate the graphite layers. Raman spectra and absorption spectra demonstrate that the obtained graphene-1-one-pyridine (GsNc) has a highly conjugated structure that facilitates electron transfer in electrochemical processes. This novel GsNc possess high sensitivity toward H2O2 (1157.1 μA mM−1 cm−2) within a linear concentration range of 1 μM to 5 mM. The proposed enzyme-free H2O2 sensor exhibits high selectivity and excellent stability. Furthermore, a choline biosensor is developed for detecting H2O2 released in bioreactions. In this biosensor, positively charged GsNc provides optimal conditions for immobilizing choline oxidase (ChOx) through electrostatic interactions. This immobilization retains 83.4% of ChOx bioactivity and is thus excellent for choline determination. This modified electrode has high potential for application in oxidase-based biosensors.
AB - A one-step process combining chemical modification and exfoliation for obtaining a graphene (Gs) derivative from pristine graphite is conducted through Friedel–Crafts chemical acylation. This electrophilic substitution reaction results in the carboxylic group (–COOH) of niacin transforming into an acylium ion (–C+[dbnd]O), which is an active species that replaces the sp2 C[sbnd]H of graphite. In addition, highly viscous polyphosphoric acid under mechanical stirring was used to exert strong shear stress on and consequently exfoliate the graphite layers. Raman spectra and absorption spectra demonstrate that the obtained graphene-1-one-pyridine (GsNc) has a highly conjugated structure that facilitates electron transfer in electrochemical processes. This novel GsNc possess high sensitivity toward H2O2 (1157.1 μA mM−1 cm−2) within a linear concentration range of 1 μM to 5 mM. The proposed enzyme-free H2O2 sensor exhibits high selectivity and excellent stability. Furthermore, a choline biosensor is developed for detecting H2O2 released in bioreactions. In this biosensor, positively charged GsNc provides optimal conditions for immobilizing choline oxidase (ChOx) through electrostatic interactions. This immobilization retains 83.4% of ChOx bioactivity and is thus excellent for choline determination. This modified electrode has high potential for application in oxidase-based biosensors.
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U2 - 10.1016/j.snb.2016.09.044
DO - 10.1016/j.snb.2016.09.044
M3 - Article
AN - SCOPUS:84990929583
SN - 0925-4005
VL - 240
SP - 1153
EP - 1159
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -