Facile preparation of polydiacetylene-based uniform porous fluorescent microspheres for potential immunoassay applications

Wei Zhang, Yun Chen, Ya Shao, Li Juan Fan

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Fluorescent microspheres are prepared by attaching self-assembled polydiacetylene (PDA) vesicles with carboxyl side groups onto the substrate amino-modified poly(glycidylmethacrylate) (APGMA) microspheres. The characterization by SEM, confocal microscopy and flow cytometry demonstrated that the final resulting microspheres are highly uniform both in size (with a diameter of 5 μm) and in fluorescence emission (coefficient of variance < 3%). The Brunauer-Emmett-Teller (BET) surface area of these spheres is 114 m 2 g -1 . In addition, there are evenly distributed pores with an average size of 20.6 nm on the spheres. These spheres are found to have good thermal stability and photostability, and do not suffer from fluorophore leaching. Fluorescein isothiocyanate (FITC) labelled bovine serum albumin (BSA) as a representative biomolecule can be easily attached onto the fluorescent microspheres. All these characteristics possessed by the APGMA-PDA spheres allow them to be directly used as carriers of biomolecules in lab-on-a-chip immunoassay systems. This journal is

Original languageEnglish
Pages (from-to)5249-5255
Number of pages7
JournalJournal of Materials Chemistry B
Volume2
Issue number32
DOIs
Publication statusPublished - 2014 Aug 28

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Microspheres
Biomolecules
Lab-on-a-chip
Flow cytometry
Fluorophores
Confocal microscopy
Bovine Serum Albumin
Fluorescein
Leaching
Thermodynamic stability
Fluorescence
Scanning electron microscopy
polydiacetylene
Substrates

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)

Cite this

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abstract = "Fluorescent microspheres are prepared by attaching self-assembled polydiacetylene (PDA) vesicles with carboxyl side groups onto the substrate amino-modified poly(glycidylmethacrylate) (APGMA) microspheres. The characterization by SEM, confocal microscopy and flow cytometry demonstrated that the final resulting microspheres are highly uniform both in size (with a diameter of 5 μm) and in fluorescence emission (coefficient of variance < 3{\%}). The Brunauer-Emmett-Teller (BET) surface area of these spheres is 114 m 2 g -1 . In addition, there are evenly distributed pores with an average size of 20.6 nm on the spheres. These spheres are found to have good thermal stability and photostability, and do not suffer from fluorophore leaching. Fluorescein isothiocyanate (FITC) labelled bovine serum albumin (BSA) as a representative biomolecule can be easily attached onto the fluorescent microspheres. All these characteristics possessed by the APGMA-PDA spheres allow them to be directly used as carriers of biomolecules in lab-on-a-chip immunoassay systems. This journal is",
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Facile preparation of polydiacetylene-based uniform porous fluorescent microspheres for potential immunoassay applications. / Zhang, Wei; Chen, Yun; Shao, Ya; Fan, Li Juan.

In: Journal of Materials Chemistry B, Vol. 2, No. 32, 28.08.2014, p. 5249-5255.

Research output: Contribution to journalArticle

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