Fast and improved bioimaging via temporal focusing multiphoton excitation microscopy with binary digital-micromirror-device holography

Yong Da Sie, Chia-Yuan Chang, Chun Yu Lin, Nan-Shan Chang, Paul J. Campagnola, Shean Jen Chen

Research output: Contribution to journalArticle

Abstract

Conventional temporal focusing-based multiphoton excitation microscopy (TFMPEM) can offer widefield optical sectioning with an axial excitation confinement of a few microns. To improve the axial confinement of TFMPEM, a binary computer-generated Fourier hologram (CGFH) via a digital-micromirror-device (DMD) was implemented to intrinsically improve the axial confinement by filling the back-focal aperture of the objective lens. Experimental results show that the excitation focal volume can be condensed and the axial confinement improved about 24% according to the DMD holography. In addition, pseudouniform MPE can be achieved using two complementary CGFHs with rapid pulse-width modulation switching via the DMD. Furthermore, bioimaging of CV-1 in origin with SV40 genes-7 cells demonstrates that the TFMPEM with binary DMD holography can improve image quality by enhancing axial excitation confinement and rejecting out-of-focus excitation.

Original languageEnglish
Article number116502
JournalJournal of Biomedical Optics
Volume23
Issue number11
DOIs
Publication statusPublished - 2018 Jan 1

Fingerprint

Digital devices
Holography
holography
Microscopic examination
microscopy
excitation
Holograms
Pulse width modulation
Image quality
Lenses
Genes
pulse duration modulation
genes
apertures
lenses
cells

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Cite this

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title = "Fast and improved bioimaging via temporal focusing multiphoton excitation microscopy with binary digital-micromirror-device holography",
abstract = "Conventional temporal focusing-based multiphoton excitation microscopy (TFMPEM) can offer widefield optical sectioning with an axial excitation confinement of a few microns. To improve the axial confinement of TFMPEM, a binary computer-generated Fourier hologram (CGFH) via a digital-micromirror-device (DMD) was implemented to intrinsically improve the axial confinement by filling the back-focal aperture of the objective lens. Experimental results show that the excitation focal volume can be condensed and the axial confinement improved about 24{\%} according to the DMD holography. In addition, pseudouniform MPE can be achieved using two complementary CGFHs with rapid pulse-width modulation switching via the DMD. Furthermore, bioimaging of CV-1 in origin with SV40 genes-7 cells demonstrates that the TFMPEM with binary DMD holography can improve image quality by enhancing axial excitation confinement and rejecting out-of-focus excitation.",
author = "Sie, {Yong Da} and Chia-Yuan Chang and Lin, {Chun Yu} and Nan-Shan Chang and Campagnola, {Paul J.} and Chen, {Shean Jen}",
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Fast and improved bioimaging via temporal focusing multiphoton excitation microscopy with binary digital-micromirror-device holography. / Sie, Yong Da; Chang, Chia-Yuan; Lin, Chun Yu; Chang, Nan-Shan; Campagnola, Paul J.; Chen, Shean Jen.

In: Journal of Biomedical Optics, Vol. 23, No. 11, 116502, 01.01.2018.

Research output: Contribution to journalArticle

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AU - Lin, Chun Yu

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AU - Campagnola, Paul J.

AU - Chen, Shean Jen

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