Fast DNA hybridization chip by controling temperature and velocity of nucleic acids

Yung Chiang Chung, Yu-Cheng Lin, Wei Chieh Liao, Po Wen Chen, Fong Jian Sie, Chia Ming Hu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrate that the efficiency of DNA hybridization could be improved by introducing elevated temperature in the hot region and higher velocities in the cold region of a microfluidic chip. Compared with the conventional methods, this hybridization microchip was shown to increase the hybridization signal 4.6-fold within 30 minutes using a 1.4 kb target DNA as the test material. The increase in fluorescence intensity was apparent when the temperature was higher than 82°C, and the fluorescence intensity reached an asymptotic value as T > 90°C. A mathematical model was proposed to relate the fluorescence intensity of DNA hybridization with the temperature of hot region and the velocity of cold region. Based on these results, the new hybridization chip with the processes of temperature and velocity differences will provide additional efficiency in DNA detection.

Original languageEnglish
Title of host publication2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009
Pages108-111
Number of pages4
DOIs
Publication statusPublished - 2009
Event2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009 - Tainan, Taiwan
Duration: 2009 Oct 182009 Oct 21

Other

Other2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009
CountryTaiwan
CityTainan
Period09-10-1809-10-21

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology

Fingerprint Dive into the research topics of 'Fast DNA hybridization chip by controling temperature and velocity of nucleic acids'. Together they form a unique fingerprint.

  • Cite this

    Chung, Y. C., Lin, Y-C., Liao, W. C., Chen, P. W., Sie, F. J., & Hu, C. M. (2009). Fast DNA hybridization chip by controling temperature and velocity of nucleic acids. In 2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009 (pp. 108-111). [5559109] https://doi.org/10.1109/NANOMED.2009.5559109