Fast DNA hybridization chip by controling temperature and velocity of nucleic acids

Yung Chiang Chung; Yu-Cheng Lin; Wei Chieh Liao; Po Wen Chen; Fong Jian Sie; Chia Ming Hu

doi:10.1109/NANOMED.2009.5559109

Fast DNA hybridization chip by controling temperature and velocity of nucleic acids

Yung Chiang Chung, Yu-Cheng Lin, Wei Chieh Liao, Po Wen Chen, Fong Jian Sie, Chia Ming Hu

Department of Engineering Science

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We demonstrate that the efficiency of DNA hybridization could be improved by introducing elevated temperature in the hot region and higher velocities in the cold region of a microfluidic chip. Compared with the conventional methods, this hybridization microchip was shown to increase the hybridization signal 4.6-fold within 30 minutes using a 1.4 kb target DNA as the test material. The increase in fluorescence intensity was apparent when the temperature was higher than 82°C, and the fluorescence intensity reached an asymptotic value as T > 90°C. A mathematical model was proposed to relate the fluorescence intensity of DNA hybridization with the temperature of hot region and the velocity of cold region. Based on these results, the new hybridization chip with the processes of temperature and velocity differences will provide additional efficiency in DNA detection.

Original language	English
Title of host publication	2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009
Pages	108-111
Number of pages	4
DOIs	https://doi.org/10.1109/NANOMED.2009.5559109
Publication status	Published - 2009
Event	2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009 - Tainan, Taiwan Duration: 2009 Oct 18 → 2009 Oct 21

Other

Other	2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009
Country/Territory	Taiwan
City	Tainan
Period	09-10-18 → 09-10-21

All Science Journal Classification (ASJC) codes

Molecular Medicine
Pharmacology

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10.1109/NANOMED.2009.5559109

Cite this

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title = "Fast DNA hybridization chip by controling temperature and velocity of nucleic acids",

abstract = "We demonstrate that the efficiency of DNA hybridization could be improved by introducing elevated temperature in the hot region and higher velocities in the cold region of a microfluidic chip. Compared with the conventional methods, this hybridization microchip was shown to increase the hybridization signal 4.6-fold within 30 minutes using a 1.4 kb target DNA as the test material. The increase in fluorescence intensity was apparent when the temperature was higher than 82°C, and the fluorescence intensity reached an asymptotic value as T > 90°C. A mathematical model was proposed to relate the fluorescence intensity of DNA hybridization with the temperature of hot region and the velocity of cold region. Based on these results, the new hybridization chip with the processes of temperature and velocity differences will provide additional efficiency in DNA detection.",

author = "Chung, {Yung Chiang} and Yu-Cheng Lin and Liao, {Wei Chieh} and Chen, {Po Wen} and Sie, {Fong Jian} and Hu, {Chia Ming}",

year = "2009",

doi = "10.1109/NANOMED.2009.5559109",

language = "English",

isbn = "9781424455287",

pages = "108--111",

booktitle = "2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009",

note = "2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009 ; Conference date: 18-10-2009 Through 21-10-2009",

}

Chung, YC, Lin, Y-C, Liao, WC, Chen, PW, Sie, FJ & Hu, CM 2009, Fast DNA hybridization chip by controling temperature and velocity of nucleic acids. in 2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009., 5559109, pp. 108-111, 2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009, Tainan, Taiwan, 09-10-18. https://doi.org/10.1109/NANOMED.2009.5559109

TY - GEN

T1 - Fast DNA hybridization chip by controling temperature and velocity of nucleic acids

AU - Chung, Yung Chiang

AU - Lin, Yu-Cheng

AU - Liao, Wei Chieh

AU - Chen, Po Wen

AU - Sie, Fong Jian

AU - Hu, Chia Ming

PY - 2009

Y1 - 2009

N2 - We demonstrate that the efficiency of DNA hybridization could be improved by introducing elevated temperature in the hot region and higher velocities in the cold region of a microfluidic chip. Compared with the conventional methods, this hybridization microchip was shown to increase the hybridization signal 4.6-fold within 30 minutes using a 1.4 kb target DNA as the test material. The increase in fluorescence intensity was apparent when the temperature was higher than 82°C, and the fluorescence intensity reached an asymptotic value as T > 90°C. A mathematical model was proposed to relate the fluorescence intensity of DNA hybridization with the temperature of hot region and the velocity of cold region. Based on these results, the new hybridization chip with the processes of temperature and velocity differences will provide additional efficiency in DNA detection.

AB - We demonstrate that the efficiency of DNA hybridization could be improved by introducing elevated temperature in the hot region and higher velocities in the cold region of a microfluidic chip. Compared with the conventional methods, this hybridization microchip was shown to increase the hybridization signal 4.6-fold within 30 minutes using a 1.4 kb target DNA as the test material. The increase in fluorescence intensity was apparent when the temperature was higher than 82°C, and the fluorescence intensity reached an asymptotic value as T > 90°C. A mathematical model was proposed to relate the fluorescence intensity of DNA hybridization with the temperature of hot region and the velocity of cold region. Based on these results, the new hybridization chip with the processes of temperature and velocity differences will provide additional efficiency in DNA detection.

UR - http://www.scopus.com/inward/record.url?scp=77957992143&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77957992143&partnerID=8YFLogxK

U2 - 10.1109/NANOMED.2009.5559109

DO - 10.1109/NANOMED.2009.5559109

M3 - Conference contribution

AN - SCOPUS:77957992143

SN - 9781424455287

SP - 108

EP - 111

BT - 2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009

T2 - 2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009

Y2 - 18 October 2009 through 21 October 2009

ER -

Fast DNA hybridization chip by controling temperature and velocity of nucleic acids

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