Fast DNA hybridization chip using denature and motion of nucleic acids

Yung Chiang Chung; Yu-Cheng Lin; Chaung Di Chueh; Chuan You Ye; Li Wei Lai; Wei Chieh Liao

doi:10.1109/IMPACT.2010.5699612

Fast DNA hybridization chip using denature and motion of nucleic acids

Yung Chiang Chung, Yu-Cheng Lin, Chaung Di Chueh, Chuan You Ye, Li Wei Lai, Wei Chieh Liao

Department of Engineering Science

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We demonstrate that the efficiency of DNA hybridization could be improved by introducing elevated temperature in the hot region and higher velocities in the cold region of a microfluidic chip. Compared with the conventional methods, this hybridization microchip was shown to increase the hybridization signal 4.6-fold within 30 minutes using a 1.4 kb target DNA as the test material. The increase in fluorescence intensity was apparent when the temperature was higher than 82°C, and the fluorescence intensity reached an asymptotic value as T > 90°C. A mathematical model was proposed to relate the fluorescence intensity of DNA hybridization with the temperature of hot region and the velocity of cold region. Based on these results, the new hybridization chip with the processes of temperature and velocity differences will provide additional efficiency in DNA detection.

Original language	English
Title of host publication	International Microsystems Packaging Assembly and Circuits Technology Conference, IMPACT 2010 and International 3D IC Conference, Proceedings
DOIs	https://doi.org/10.1109/IMPACT.2010.5699612
Publication status	Published - 2010
Event	2010 5th International Microsystems, Packaging, Assembly and Circuits Technology Conference, IMPACT 2010 and International 3D IC Conference - Taipei, Taiwan Duration: 2010 Oct 20 → 2010 Oct 22

Other

Other	2010 5th International Microsystems, Packaging, Assembly and Circuits Technology Conference, IMPACT 2010 and International 3D IC Conference
Country	Taiwan
City	Taipei
Period	10-10-20 → 10-10-22

All Science Journal Classification (ASJC) codes

Hardware and Architecture
Electrical and Electronic Engineering

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title = "Fast DNA hybridization chip using denature and motion of nucleic acids",

abstract = "We demonstrate that the efficiency of DNA hybridization could be improved by introducing elevated temperature in the hot region and higher velocities in the cold region of a microfluidic chip. Compared with the conventional methods, this hybridization microchip was shown to increase the hybridization signal 4.6-fold within 30 minutes using a 1.4 kb target DNA as the test material. The increase in fluorescence intensity was apparent when the temperature was higher than 82°C, and the fluorescence intensity reached an asymptotic value as T > 90°C. A mathematical model was proposed to relate the fluorescence intensity of DNA hybridization with the temperature of hot region and the velocity of cold region. Based on these results, the new hybridization chip with the processes of temperature and velocity differences will provide additional efficiency in DNA detection.",

author = "Chung, {Yung Chiang} and Yu-Cheng Lin and Chueh, {Chaung Di} and Ye, {Chuan You} and Lai, {Li Wei} and Liao, {Wei Chieh}",

year = "2010",

doi = "10.1109/IMPACT.2010.5699612",

language = "English",

isbn = "9781424497836",

booktitle = "International Microsystems Packaging Assembly and Circuits Technology Conference, IMPACT 2010 and International 3D IC Conference, Proceedings",

note = "2010 5th International Microsystems, Packaging, Assembly and Circuits Technology Conference, IMPACT 2010 and International 3D IC Conference ; Conference date: 20-10-2010 Through 22-10-2010",

}

Chung, YC, Lin, Y-C, Chueh, CD, Ye, CY, Lai, LW & Liao, WC 2010, Fast DNA hybridization chip using denature and motion of nucleic acids. in International Microsystems Packaging Assembly and Circuits Technology Conference, IMPACT 2010 and International 3D IC Conference, Proceedings., 5699612, 2010 5th International Microsystems, Packaging, Assembly and Circuits Technology Conference, IMPACT 2010 and International 3D IC Conference, Taipei, Taiwan, 10-10-20. https://doi.org/10.1109/IMPACT.2010.5699612

Fast DNA hybridization chip using denature and motion of nucleic acids. / Chung, Yung Chiang; Lin, Yu-Cheng; Chueh, Chaung Di; Ye, Chuan You; Lai, Li Wei; Liao, Wei Chieh.

International Microsystems Packaging Assembly and Circuits Technology Conference, IMPACT 2010 and International 3D IC Conference, Proceedings. 2010. 5699612.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Fast DNA hybridization chip using denature and motion of nucleic acids

AU - Chung, Yung Chiang

AU - Lin, Yu-Cheng

AU - Chueh, Chaung Di

AU - Ye, Chuan You

AU - Lai, Li Wei

AU - Liao, Wei Chieh

PY - 2010

Y1 - 2010

N2 - We demonstrate that the efficiency of DNA hybridization could be improved by introducing elevated temperature in the hot region and higher velocities in the cold region of a microfluidic chip. Compared with the conventional methods, this hybridization microchip was shown to increase the hybridization signal 4.6-fold within 30 minutes using a 1.4 kb target DNA as the test material. The increase in fluorescence intensity was apparent when the temperature was higher than 82°C, and the fluorescence intensity reached an asymptotic value as T > 90°C. A mathematical model was proposed to relate the fluorescence intensity of DNA hybridization with the temperature of hot region and the velocity of cold region. Based on these results, the new hybridization chip with the processes of temperature and velocity differences will provide additional efficiency in DNA detection.

AB - We demonstrate that the efficiency of DNA hybridization could be improved by introducing elevated temperature in the hot region and higher velocities in the cold region of a microfluidic chip. Compared with the conventional methods, this hybridization microchip was shown to increase the hybridization signal 4.6-fold within 30 minutes using a 1.4 kb target DNA as the test material. The increase in fluorescence intensity was apparent when the temperature was higher than 82°C, and the fluorescence intensity reached an asymptotic value as T > 90°C. A mathematical model was proposed to relate the fluorescence intensity of DNA hybridization with the temperature of hot region and the velocity of cold region. Based on these results, the new hybridization chip with the processes of temperature and velocity differences will provide additional efficiency in DNA detection.

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SN - 9781424497836

BT - International Microsystems Packaging Assembly and Circuits Technology Conference, IMPACT 2010 and International 3D IC Conference, Proceedings

T2 - 2010 5th International Microsystems, Packaging, Assembly and Circuits Technology Conference, IMPACT 2010 and International 3D IC Conference

Y2 - 20 October 2010 through 22 October 2010

ER -

Fast DNA hybridization chip using denature and motion of nucleic acids

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