Fast separation of bromelain by polyacrylic acid-bound iron oxide magnetic nanoparticles

Dong-Hwang Chen, Shih Hung Huang

Research output: Contribution to journalArticle

42 Citations (Scopus)


The adsorption of bromelain from an aqueous solution by polyacrylic acid (PAA)-bound iron oxide magnetic nanoparticles was studied. The magnetic composite nanoparticles were shown to be efficient for the separation of bromelain. Except at pH <3, the adsorption of bromelain increased with the decrease in solution pH and reached almost 100% at pH 3-5. The adsorbed bromelain could be desorbed by the addition of KCl and complete desorption was achieved at pH 7 when [KCl]>0.6 M. The adsorption behaviour followed the Langmuir isotherm with a maximum adsorption amount of 0.476 mg/mg and a Langmuir adsorption equilibrium constant of 58.4 ml/mg at pH 4 and 0.1 M phosphate. In addition, it was notable that both the adsorption and desorption of bromelain were quite fast and could be completed in about 1 min due to the absence of internal diffusion resistance. Bromelain retained 87.4% activity after adsorption/desorption.

Original languageEnglish
Pages (from-to)2207-2211
Number of pages5
JournalProcess Biochemistry
Issue number12
Publication statusPublished - 2004 Oct 29

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry
  • Engineering (miscellaneous)
  • Industrial and Manufacturing Engineering
  • Applied Microbiology and Biotechnology
  • Bioengineering

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