Fast volumetric imaging with patterned illumination via digital micro-mirror devicebased temporal focusing multiphoton microscopy

Chia Yuan Chang, Yvonne Yuling Hu, Chun Yu Lin, Cheng Han Lin, Hsin Yu Chang, Sheng Feng Tsai, Tzu Wei Lin, Shean Jen Chen

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Temporal focusing multiphoton microscopy (TFMPM) has the advantage of area excitation in an axial confinement of only a few microns; hence, it can offer fast three-dimensional (3D) multiphoton imaging. Herein, fast volumetric imaging via a developed digital micromirror device (DMD)-based TFMPM has been realized through the synchronization of an electron multiplying charge-coupled device (EMCCD) with a dynamic piezoelectric stage for axial scanning. The volumetric imaging rate can achieve 30 volumes per second according to the EMCCD frame rate of more than 400 frames per second, which allows for the 3D Brownian motion of one-micron fluorescent beads to be spatially observed. Furthermore, it is demonstrated that the dynamic HiLo structural multiphoton microscope can reject background noise by way of the fast volumetric imaging with high-speed DMD patterned illumination.

Original languageEnglish
Pages (from-to)1727-1736
Number of pages10
JournalBiomedical Optics Express
Volume7
Issue number5
DOIs
Publication statusPublished - 2016 May 1

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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