TY - JOUR
T1 - Feasibility of rapid quantitation of stratum corneum lipid content by Fourier transform infrared spectrometry
AU - Tsai, Jui Chen
AU - Lo, Yu Li
AU - Lin, Ching Yu
AU - Sheu, Hamm Ming
AU - Lin, Jui Che
N1 - Funding Information:
Tsai Jui-Chen [email protected] 1 Lo Yu-Li 2 Lin Ching-Yu 1 5 Sheu Hamm-Ming 3 Lin Jui-Che 4 1 Institute of Clinical Pharmacy College of Medicine National Cheng Kung University Tainan 701 Taiwan 2 Department of Pharmacy Chia‒Nan University of Pharmacy and Science Tainan Hsien 717 Taiwan 3 Department of Dermatology College of Medicine Taiwan 4 Department of Chemical Engineering College of Engineering National Cheng Kung University 1 University Rd. Tainan 701 Taiwan 5 Pfizer Limited Tamsui Taiwan 2004 18 3 423 431 2004 Copyright © 2004 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The permeability barrier of skin resides in the stratum corneum, and its properties are mediated by a series of lipid multilayers, enriched in ceramides, cholesterol, and free fatty acids, segregated within the stratum corneum (SC) interstices. SC lipid content is usually determined by gravimetric methods in conjunction with high performance thin layer chromatography, but these methods are time‒consuming and involve hazardous solvents. The objective of the present study was to develop a method of measuring SC lipid content by Fourier transform infrared spectrometry (FTIR) that is fast and requires no solvents. The IR spectra of isolated porcine SC sheets were recorded using a FTIR spectrometer. SC lipid content was determined by gravimetric methods using chloroform–methanol extraction. The peak area of both the CH 2 symmetric (2850 cm −1 ) and asymmetric (2920 cm −1 ) stretching bands in the IR spectra of progressively solvent‒extracted porcine SC sheets decreased with increasing amount of SC lipids removed. When spectral analysis was performed by curve‒fitting using GRAMS/32 software between 3000 to 2800 cm −1 , peak area ratios of CH 2 to CH 3 asymmetric stretching bands in the IR spectra of 46 isolated porcine SC samples were correlated to SC lipid content ( R 2 =0.90), with the standard error of measurement of 1.91%. The study demonstrated the feasibility of using FTIR technique to rapidly and accurately measure SC lipid content. Stratum corneum infrared spectrometry lipid content quantitation deconvolution http://dx.doi.org/10.13039/501100001868 National Science Council Taiwan NSC 89-2320-B006-145
PY - 2004
Y1 - 2004
N2 - The permeability barrier of skin resides in the stratum corneum, and its properties are mediated by a series of lipid multilayers, enriched in ceramides, cholesterol, and free fatty acids, segregated within the stratum corneum (SC) interstices. SC lipid content is usually determined by gravimetric methods in conjunction with high performance thin layer chromatography, but these methods are time-consuming and involve hazardous solvents. The objective of the present study was to develop a method of measuring SC lipid content by Fourier transform infrared spectrometry (FTIR) that is fast and requires no solvents. The IR spectra of isolated porcine SC sheets were recorded using a FTIR spectrometer. SC lipid content was determined by gravimetric methods using chloroform-methanol extraction. The peak area of both the CH2 symmetric (2850 cm -1) and asymmetric (2920 cm-1) stretching bands in the IR spectra of progressively solvent-extracted porcine SC sheets decreased with increasing amount of SC lipids removed. When spectral analysis was performed by curve-fitting using GRAMS/32 software between 3000 to 2800 cm-1, peak area ratios of CH2 to CH3 asymmetric stretching bands in the IR spectra of 46 isolated porcine SC samples were correlated to SC lipid content (R2 = 0.90), with the standard error of measurement of 1.91%. The study demonstrated the feasibility of using FTIR technique to rapidly and accurately measure SC lipid content.
AB - The permeability barrier of skin resides in the stratum corneum, and its properties are mediated by a series of lipid multilayers, enriched in ceramides, cholesterol, and free fatty acids, segregated within the stratum corneum (SC) interstices. SC lipid content is usually determined by gravimetric methods in conjunction with high performance thin layer chromatography, but these methods are time-consuming and involve hazardous solvents. The objective of the present study was to develop a method of measuring SC lipid content by Fourier transform infrared spectrometry (FTIR) that is fast and requires no solvents. The IR spectra of isolated porcine SC sheets were recorded using a FTIR spectrometer. SC lipid content was determined by gravimetric methods using chloroform-methanol extraction. The peak area of both the CH2 symmetric (2850 cm -1) and asymmetric (2920 cm-1) stretching bands in the IR spectra of progressively solvent-extracted porcine SC sheets decreased with increasing amount of SC lipids removed. When spectral analysis was performed by curve-fitting using GRAMS/32 software between 3000 to 2800 cm-1, peak area ratios of CH2 to CH3 asymmetric stretching bands in the IR spectra of 46 isolated porcine SC samples were correlated to SC lipid content (R2 = 0.90), with the standard error of measurement of 1.91%. The study demonstrated the feasibility of using FTIR technique to rapidly and accurately measure SC lipid content.
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U2 - 10.1155/2004/401015
DO - 10.1155/2004/401015
M3 - Article
AN - SCOPUS:3943066988
SN - 0712-4813
VL - 18
SP - 423
EP - 431
JO - Spectroscopy
JF - Spectroscopy
IS - 3
ER -