TY - JOUR
T1 - Fermentation strategies for the production of lipase by an indigenous isolate Burkholderia sp. C20
AU - Liu, Chien Hung
AU - Chen, Chun Yen
AU - Wang, Yao Wen
AU - Chang, Jo Shu
N1 - Funding Information:
The authors gratefully acknowledge the financial support from Taiwan's National Science Council under grant number NSC 98-2221-E-006-240-MY3 .
PY - 2011/12/15
Y1 - 2011/12/15
N2 - Burkholderia sp. C20 strain isolated from food wastes produces a lipase with hydrolytic activities towards olive oil. Fermentation strategies for efficient production of this Burkholderia lipase were developed using a 5-L bench top bioreactor. Critical factors affecting the fermentative lipase production were examined, including pH, aeration rate, agitation rate, and incubation time. Adjusting the aeration rate from 0.5 to 2. vvm gave an increase in the overall lipase productivity from 0.057 to 0.076. U/(ml. h), which was further improved to 0.09. U/(ml. h) by adjusting the agitation speed to 100. rpm. The production of Burkholderia lipase followed mixed growth-associated kinetics with a yield coefficient of 524. U/g-dry-cell-weight. The pH optimum for cell growth and lipase production was different at 7.0 and 6.0, respectively. Furthermore, stepwise addition of carbon substrate (i.e., olive oil) enhanced lipase production in both flask and bioreactor experiments.
AB - Burkholderia sp. C20 strain isolated from food wastes produces a lipase with hydrolytic activities towards olive oil. Fermentation strategies for efficient production of this Burkholderia lipase were developed using a 5-L bench top bioreactor. Critical factors affecting the fermentative lipase production were examined, including pH, aeration rate, agitation rate, and incubation time. Adjusting the aeration rate from 0.5 to 2. vvm gave an increase in the overall lipase productivity from 0.057 to 0.076. U/(ml. h), which was further improved to 0.09. U/(ml. h) by adjusting the agitation speed to 100. rpm. The production of Burkholderia lipase followed mixed growth-associated kinetics with a yield coefficient of 524. U/g-dry-cell-weight. The pH optimum for cell growth and lipase production was different at 7.0 and 6.0, respectively. Furthermore, stepwise addition of carbon substrate (i.e., olive oil) enhanced lipase production in both flask and bioreactor experiments.
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U2 - 10.1016/j.bej.2011.09.001
DO - 10.1016/j.bej.2011.09.001
M3 - Article
AN - SCOPUS:80054831779
SN - 1369-703X
VL - 58-59
SP - 96
EP - 102
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
IS - 1
ER -