TY - JOUR
T1 - Fermentative biohydrogen production from lactate and acetate
AU - Wu, Chao Wei
AU - Whang, Liang Ming
AU - Cheng, Hai Hsuan
AU - Chan, Kan Chi
N1 - Funding Information:
The authors would like to acknowledge the financial support from the National Science Council of Taiwan under Grant NSC 98-3114-E-006-013 and NSC100-3113-E-006-017, and NSC 101-3113-E-006-016.
PY - 2012/6
Y1 - 2012/6
N2 - In this study, a continuous-flow stirred tank reactor (CSTR) fed with lactate and acetate was operated to enrich hydrogen-producing bacteria. By varying the influent substrate concentrations and hydraulic retention times (HRT), the volumetric loading rate (VLR) of 55.64kg-COD/m 3/day seemed to be optimum for this enriched culture for fermentative hydrogen production from lactate and acetate. The results of batch experiments confirmed that the enriched culture tended to fulfill the e - equiv requirement for cell growth at a lower VLR condition (21.77kg-COD/m 3/day), while it could largely distribute the e - equiv for hydrogen production at a higher VLR condition. However, a maximum lactate/acetate concentration allowed for enriching this culture existed, especially at a lower HRT condition in which wash-out can be an issue for this enriched culture. Finally, the results of cloning and sequencing indicated that Clostridium tyrobutyricum was considered the major hydrogen-producing bacteria in the CSTR fed with lactate and acetate.
AB - In this study, a continuous-flow stirred tank reactor (CSTR) fed with lactate and acetate was operated to enrich hydrogen-producing bacteria. By varying the influent substrate concentrations and hydraulic retention times (HRT), the volumetric loading rate (VLR) of 55.64kg-COD/m 3/day seemed to be optimum for this enriched culture for fermentative hydrogen production from lactate and acetate. The results of batch experiments confirmed that the enriched culture tended to fulfill the e - equiv requirement for cell growth at a lower VLR condition (21.77kg-COD/m 3/day), while it could largely distribute the e - equiv for hydrogen production at a higher VLR condition. However, a maximum lactate/acetate concentration allowed for enriching this culture existed, especially at a lower HRT condition in which wash-out can be an issue for this enriched culture. Finally, the results of cloning and sequencing indicated that Clostridium tyrobutyricum was considered the major hydrogen-producing bacteria in the CSTR fed with lactate and acetate.
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U2 - 10.1016/j.biortech.2011.12.130
DO - 10.1016/j.biortech.2011.12.130
M3 - Article
C2 - 22318084
AN - SCOPUS:84860227186
SN - 0960-8524
VL - 113
SP - 30
EP - 36
JO - Bioresource technology
JF - Bioresource technology
ER -
