Electrospinning has been attributed to be one of the most effective method to prepare nano-fibers, and widely applied in assorted fields. The nanofiber membranes made by electrospinning feature high porosity and surface area, and are qualified for vascular grafts, biological scaffolds, and wound dressings. Chitosan is non-toxic and biodegradable, making it a good biocompatible material; in addition, it is also proved to be anti-bacterial and help cell growth in wounds. This research produced nanofiber membrane with polyethylene oxide (PEO) by electrospinning; the influence of the three parameters -mixture ratio of solution, electric field, and distance between the capillary tip and the collecting plate, on electrospinning was then explored. According to the results of the experiment, electrospinning formed the optimum nanofibers when the volume mixing ratio of PEO/chitosan was 60:40.