Abstract
Oxidative stress and lower levels of trophic factors involved in nigrostriatal dopaminergic neurodegeneration are a hallmark of Parkinson disease. Our previous studies found that fibroblast growth factor 9 (FGF9) prevented 1-methyl-4-phenylpyridinium (MPP+)-induced nigral dopaminergic neuron death and was involved in the neuroprotection of the antioxidant melatonin. However, the protective mechanisms mediated by FGF9 remain unclear. Herein, we explored whether FGF9 regulated the cellular antioxidant defense protecting dopaminergic neurons against MPP+ intoxication. We found that FGF9 treatment alone induced a decrease in hydrogen peroxide (H2O2) level, an increase in glutathione content, and an upregulation of γ-glutamylcysteine synthetase (γ-GCS) and heme oxygenase 1 (HO-1) expression in primary cortical neurons but not in astrocytes. Simultaneous treatment with FGF9 and MPP+ prevented MPP+-induced neuron death and H2O2 overproduction but did not affect the FGF9-increased γ-GCS and HO-1 protein expression. Inhibition of γ-GCS or HO-1 prevented the inhibitory effect of FGF9 on MPP+-induced H2O2 production and death in mesencephalic dopaminergic and cortical neurons. However, in the absence of MPP+, the FGF9-induced H2O2 reduction was blocked by HO-1 inhibitors, but not by γ-GCS inhibitors. These results indicate that FGF9 upregulates γ-GCS and HO-1 expression to protect cortical and dopaminergic neurons from MPP+-induced oxidative insult.
| Original language | English |
|---|---|
| Pages (from-to) | 1099-1108 |
| Number of pages | 10 |
| Journal | Free Radical Biology and Medicine |
| Volume | 49 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2010 Sep 1 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Physiology (medical)
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Fibroblast growth factor 9 upregulates heme oxygenase-1 and γ-glutamylcysteine synthetase expression to protect neurons from 1-methyl-4-phenylpyridinium toxicity. / Huang, Jui Yen; Chuang, Jih Ing.
In: Free Radical Biology and Medicine, Vol. 49, No. 6, 01.09.2010, p. 1099-1108.Research output: Contribution to journal › Article
TY - JOUR
T1 - Fibroblast growth factor 9 upregulates heme oxygenase-1 and γ-glutamylcysteine synthetase expression to protect neurons from 1-methyl-4-phenylpyridinium toxicity
AU - Huang, Jui Yen
AU - Chuang, Jih Ing
PY - 2010/9/1
Y1 - 2010/9/1
N2 - Oxidative stress and lower levels of trophic factors involved in nigrostriatal dopaminergic neurodegeneration are a hallmark of Parkinson disease. Our previous studies found that fibroblast growth factor 9 (FGF9) prevented 1-methyl-4-phenylpyridinium (MPP+)-induced nigral dopaminergic neuron death and was involved in the neuroprotection of the antioxidant melatonin. However, the protective mechanisms mediated by FGF9 remain unclear. Herein, we explored whether FGF9 regulated the cellular antioxidant defense protecting dopaminergic neurons against MPP+ intoxication. We found that FGF9 treatment alone induced a decrease in hydrogen peroxide (H2O2) level, an increase in glutathione content, and an upregulation of γ-glutamylcysteine synthetase (γ-GCS) and heme oxygenase 1 (HO-1) expression in primary cortical neurons but not in astrocytes. Simultaneous treatment with FGF9 and MPP+ prevented MPP+-induced neuron death and H2O2 overproduction but did not affect the FGF9-increased γ-GCS and HO-1 protein expression. Inhibition of γ-GCS or HO-1 prevented the inhibitory effect of FGF9 on MPP+-induced H2O2 production and death in mesencephalic dopaminergic and cortical neurons. However, in the absence of MPP+, the FGF9-induced H2O2 reduction was blocked by HO-1 inhibitors, but not by γ-GCS inhibitors. These results indicate that FGF9 upregulates γ-GCS and HO-1 expression to protect cortical and dopaminergic neurons from MPP+-induced oxidative insult.
AB - Oxidative stress and lower levels of trophic factors involved in nigrostriatal dopaminergic neurodegeneration are a hallmark of Parkinson disease. Our previous studies found that fibroblast growth factor 9 (FGF9) prevented 1-methyl-4-phenylpyridinium (MPP+)-induced nigral dopaminergic neuron death and was involved in the neuroprotection of the antioxidant melatonin. However, the protective mechanisms mediated by FGF9 remain unclear. Herein, we explored whether FGF9 regulated the cellular antioxidant defense protecting dopaminergic neurons against MPP+ intoxication. We found that FGF9 treatment alone induced a decrease in hydrogen peroxide (H2O2) level, an increase in glutathione content, and an upregulation of γ-glutamylcysteine synthetase (γ-GCS) and heme oxygenase 1 (HO-1) expression in primary cortical neurons but not in astrocytes. Simultaneous treatment with FGF9 and MPP+ prevented MPP+-induced neuron death and H2O2 overproduction but did not affect the FGF9-increased γ-GCS and HO-1 protein expression. Inhibition of γ-GCS or HO-1 prevented the inhibitory effect of FGF9 on MPP+-induced H2O2 production and death in mesencephalic dopaminergic and cortical neurons. However, in the absence of MPP+, the FGF9-induced H2O2 reduction was blocked by HO-1 inhibitors, but not by γ-GCS inhibitors. These results indicate that FGF9 upregulates γ-GCS and HO-1 expression to protect cortical and dopaminergic neurons from MPP+-induced oxidative insult.
UR - http://www.scopus.com/inward/record.url?scp=77955516215&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77955516215&partnerID=8YFLogxK
U2 - 10.1016/j.freeradbiomed.2010.06.026
DO - 10.1016/j.freeradbiomed.2010.06.026
M3 - Article
C2 - 20615462
AN - SCOPUS:77955516215
VL - 49
SP - 1099
EP - 1108
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
SN - 0891-5849
IS - 6
ER -