Fibronectin in cell adhesion and migration via N-glycosylation

Cheng Te Hsiao, Hung Wei Cheng, Chi Ming Huang, Hao Ru Li, Meng Hsin Ou, Jie Rong Huang, Kay Hooi Khoo, Helen Wenshin Yu, Yin Quan Chen, Yang Kao Wang, Arthur Chiou, Jean Cheng Kuo

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Abstract

Directed cell migration is an important step in effective wound healing and requires the dynamic control of the formation of cell-extracellular matrix interactions. Plasma fibronectin is an extracellular matrix glycoprotein present in blood plasma that plays crucial roles in modulating cellular adhesion and migration and thereby helping to mediate all steps of wound healing. In order to seek safe sources of plasma fibronectin for its practical use in wound dressing, we isolated fibronectin from human (homo) and porcine plasma and demonstrated that both have a similar ability as a suitable substrate for the stimulation of cell adhesion and for directing cell migration. In addition, we also defined the N-glycosylation sites and N-glycans present on homo and porcine plasma fibronectin. These N-glycosylation modifications of the plasma fibronectin synergistically support the integrin-mediated signals to bring about mediating cellular adhesion and directed cell migration. This study not only determines the important function of N-glycans in both homo and porcine plasma fibronectin-mediated cell adhesion and directed cell migration, but also reveals the potential applications of porcine plasma fibronectin if it was applied as a material for clinical wound healing and tissue repair.

Original languageEnglish
Pages (from-to)70653-70668
Number of pages16
JournalOncotarget
Volume8
Issue number41
DOIs
Publication statusPublished - 2017 Jan 1

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All Science Journal Classification (ASJC) codes

  • Oncology

Cite this

Hsiao, C. T., Cheng, H. W., Huang, C. M., Li, H. R., Ou, M. H., Huang, J. R., Khoo, K. H., Yu, H. W., Chen, Y. Q., Wang, Y. K., Chiou, A., & Kuo, J. C. (2017). Fibronectin in cell adhesion and migration via N-glycosylation. Oncotarget, 8(41), 70653-70668. https://doi.org/10.18632/oncotarget.19969