TY - JOUR
T1 - Field-effect-transistor-based calcium ion sensors using Zn 3Ta2O5 sensing membrane
AU - Lee, Ching Ting
AU - Chiu, Ying Shuo
N1 - Funding Information:
This work was supported by the Ministry of Science and Technology Taiwan , Republic of China under MOST 103-2221-E-006-002- , NSC-102-2221-E-006-269 and the Advanced Optoelectronic Technology Center and Research Center Energy Technology and Strategy of the National Cheng Kung University.
PY - 2014/11
Y1 - 2014/11
N2 - To overcome the surface damage of ZnO-based biosensors operated in strong acidic environments, similar to the stomach environment, the Zn 3Ta2O5 stoichiometric thin films were applied in the field-effect-transistor (FET)-based calcium ion biosensors as the sensing membrane. Since the Zn3Ta2O5 sensing membrane possessed the property of high corrosion resistance and high enzyme immobilization, the resulting calcium ion biosensors can be stably operated in the strong acid environments. The sensing sensitivity of the Zn 3Ta2O5 FET-based biosensor was 17.1 μA/pCa within calcium ion concentration range from pCa = 1 to pCa = 6 under the solution of pH = 2 compared to the 12.8 μA/pCa within the pCa range from 1 to 5 of the FET-based biosensor using the Ta2O5 sensing membrane. The superior results were attributed to the better immobilization between the calmodulin (CaM) and the Zn3Ta2O5 sensing membrane. Consequently, the Michaelis-Menten constant of 1.08 mM for the Zn3Ta2O5 sensing membrane was much better than 1.45 mM of the Ta2O5 sensing membrane.
AB - To overcome the surface damage of ZnO-based biosensors operated in strong acidic environments, similar to the stomach environment, the Zn 3Ta2O5 stoichiometric thin films were applied in the field-effect-transistor (FET)-based calcium ion biosensors as the sensing membrane. Since the Zn3Ta2O5 sensing membrane possessed the property of high corrosion resistance and high enzyme immobilization, the resulting calcium ion biosensors can be stably operated in the strong acid environments. The sensing sensitivity of the Zn 3Ta2O5 FET-based biosensor was 17.1 μA/pCa within calcium ion concentration range from pCa = 1 to pCa = 6 under the solution of pH = 2 compared to the 12.8 μA/pCa within the pCa range from 1 to 5 of the FET-based biosensor using the Ta2O5 sensing membrane. The superior results were attributed to the better immobilization between the calmodulin (CaM) and the Zn3Ta2O5 sensing membrane. Consequently, the Michaelis-Menten constant of 1.08 mM for the Zn3Ta2O5 sensing membrane was much better than 1.45 mM of the Ta2O5 sensing membrane.
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U2 - 10.1016/j.snb.2014.07.055
DO - 10.1016/j.snb.2014.07.055
M3 - Article
AN - SCOPUS:84905367510
VL - 203
SP - 790
EP - 794
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
SN - 0925-4005
ER -