TY - JOUR
T1 - Fine-tuning of key parameters to enhance biomass and nutritional polyunsaturated fatty acids production from Thraustochytrium sp.
AU - Chauhan, Ajeet Singh
AU - Patel, Anil Kumar
AU - Singhania, Reeta Rani
AU - Vadrale, Akash Pralhad
AU - Chen, Chiu Wen
AU - Giri, Balendu Sheker
AU - Chang, Jo Shu
AU - Dong, Cheng Di
N1 - Publisher Copyright:
© 2023 Elsevier Ltd
PY - 2024/2
Y1 - 2024/2
N2 - The escalating demand for long-chain polyunsaturated fatty acids (PUFAs) due to their vital health effects has deepened the exploration of sustainable sources. Thraustochytrium sp. stands out as a promising platform for omega-3 and 6 PUFA production. This research strategically optimizes key parameters: temperature, salinity, pH, and G:Y:P ratio and the optimized conditions for maximum biomass, total lipid, and DHA enhancement were 28 °C, 50 %, 6, and 10:1:2 respectively. Process optimization enhanced 32.30 and 31.92 % biomass (9.88 g/L) and lipid (6.57 g/L) yield. Notably, DHA concentration experienced a substantial rise of 69.91 % (1.63 g/L), accompanied by notable increases in EPA and DPA by 82.69 % and 31.47 %, respectively. MANOVA analysis underscored the statistical significance of the optimization process (p < 0.01), with all environmental factors significantly influencing biomass and lipid data (p < 0.05), particularly impacting DHA production. Thraustochytrium sp. can be a potential source of commercial DHA production with the fine-tuning of these key process parameters.
AB - The escalating demand for long-chain polyunsaturated fatty acids (PUFAs) due to their vital health effects has deepened the exploration of sustainable sources. Thraustochytrium sp. stands out as a promising platform for omega-3 and 6 PUFA production. This research strategically optimizes key parameters: temperature, salinity, pH, and G:Y:P ratio and the optimized conditions for maximum biomass, total lipid, and DHA enhancement were 28 °C, 50 %, 6, and 10:1:2 respectively. Process optimization enhanced 32.30 and 31.92 % biomass (9.88 g/L) and lipid (6.57 g/L) yield. Notably, DHA concentration experienced a substantial rise of 69.91 % (1.63 g/L), accompanied by notable increases in EPA and DPA by 82.69 % and 31.47 %, respectively. MANOVA analysis underscored the statistical significance of the optimization process (p < 0.01), with all environmental factors significantly influencing biomass and lipid data (p < 0.05), particularly impacting DHA production. Thraustochytrium sp. can be a potential source of commercial DHA production with the fine-tuning of these key process parameters.
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U2 - 10.1016/j.biortech.2023.130252
DO - 10.1016/j.biortech.2023.130252
M3 - Article
C2 - 38145766
AN - SCOPUS:85180925861
SN - 0960-8524
VL - 394
JO - Bioresource technology
JF - Bioresource technology
M1 - 130252
ER -
