First molecular identification of Vorticella sp. from freshwater shrimps in Tainan, Taiwan

Chen Chieh Liao, Jyh wei Shin, Lih Ren Chen, Lynn Ling-Huei Huang, Wei-Chen Lin

Research output: Contribution to journalArticle

Abstract

Freshwater shrimps are the most common crustaceans kept in an aquarium. This study was a survey seeking parasites infecting cultured freshwater atyid shrimps at aquarium stores in Tainan, Taiwan. We observed that atyid shrimps were infested with Vorticella and Scutariella. Scutariella is a common shrimp parasite; thus, we focused on Vorticella infection in the atyid shrimps. Vorticella aequilata-like pop TW, a freshwater peritrich ciliate, was isolated from the atyid shrimps. The morphological characteristics were investigated using live observations. Specimens from the population showed identical arrangement of the infraciliature and identical ITS1-5.8SITS2 region sequences. The zooids are bell-shaped, 40–58 μm wide and 47–70 μm in long in vivo. The food vacuole is variable in shape and is located in the middle of the cell. ITS1-5.8S-ITS2 sequences of Vorticella aequilata-like pop TW did not match any available sequences in GenBank. Phylogenetically, Vorticella aequilata-like pop TW clusters with the other Vorticella within the family Vorticellidae and nests with Vorticella aequilata in the subclade. Above all, the morphological characteristics and molecular analyses show that the investigated Vorticella is a Vorticella aequilata-like species. The phylogenetic analyses of ciliates based on the ITS1-5.8S-ITS2 sequences reveal that the Vorticella genus consists of Vorticella morphospecies and that taxonomic revision of the genus is needed. Morphometric criteria and molecular analysis were used to describe and identify the Vorticella specie and this study presents the first molecular identification analysis of the Vorticella species in the cultured atyid shrimps in Tainan, Taiwan.

Original languageEnglish
Pages (from-to)415-422
Number of pages8
JournalInternational Journal for Parasitology: Parasites and Wildlife
Volume7
Issue number3
DOIs
Publication statusPublished - 2018 Dec 1

Fingerprint

Fresh Water
Taiwan
shrimp
Parasites
Nucleic Acid Databases
Vacuoles
aquariums
Ciliophora
Food
parasites
Infection
Population
taxonomic revisions
vacuoles
Crustacea
nests
phylogeny
infection
cells

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Animal Science and Zoology
  • Infectious Diseases

Cite this

@article{af417c1ff802494cbbf99ebe8871f100,
title = "First molecular identification of Vorticella sp. from freshwater shrimps in Tainan, Taiwan",
abstract = "Freshwater shrimps are the most common crustaceans kept in an aquarium. This study was a survey seeking parasites infecting cultured freshwater atyid shrimps at aquarium stores in Tainan, Taiwan. We observed that atyid shrimps were infested with Vorticella and Scutariella. Scutariella is a common shrimp parasite; thus, we focused on Vorticella infection in the atyid shrimps. Vorticella aequilata-like pop TW, a freshwater peritrich ciliate, was isolated from the atyid shrimps. The morphological characteristics were investigated using live observations. Specimens from the population showed identical arrangement of the infraciliature and identical ITS1-5.8SITS2 region sequences. The zooids are bell-shaped, 40–58 μm wide and 47–70 μm in long in vivo. The food vacuole is variable in shape and is located in the middle of the cell. ITS1-5.8S-ITS2 sequences of Vorticella aequilata-like pop TW did not match any available sequences in GenBank. Phylogenetically, Vorticella aequilata-like pop TW clusters with the other Vorticella within the family Vorticellidae and nests with Vorticella aequilata in the subclade. Above all, the morphological characteristics and molecular analyses show that the investigated Vorticella is a Vorticella aequilata-like species. The phylogenetic analyses of ciliates based on the ITS1-5.8S-ITS2 sequences reveal that the Vorticella genus consists of Vorticella morphospecies and that taxonomic revision of the genus is needed. Morphometric criteria and molecular analysis were used to describe and identify the Vorticella specie and this study presents the first molecular identification analysis of the Vorticella species in the cultured atyid shrimps in Tainan, Taiwan.",
author = "Liao, {Chen Chieh} and Shin, {Jyh wei} and Chen, {Lih Ren} and Huang, {Lynn Ling-Huei} and Wei-Chen Lin",
year = "2018",
month = "12",
day = "1",
doi = "10.1016/j.ijppaw.2018.10.002",
language = "English",
volume = "7",
pages = "415--422",
journal = "International Journal for Parasitology: Parasites and Wildlife",
issn = "2213-2244",
publisher = "Elsevier BV",
number = "3",

}

First molecular identification of Vorticella sp. from freshwater shrimps in Tainan, Taiwan. / Liao, Chen Chieh; Shin, Jyh wei; Chen, Lih Ren; Huang, Lynn Ling-Huei; Lin, Wei-Chen.

In: International Journal for Parasitology: Parasites and Wildlife, Vol. 7, No. 3, 01.12.2018, p. 415-422.

Research output: Contribution to journalArticle

TY - JOUR

T1 - First molecular identification of Vorticella sp. from freshwater shrimps in Tainan, Taiwan

AU - Liao, Chen Chieh

AU - Shin, Jyh wei

AU - Chen, Lih Ren

AU - Huang, Lynn Ling-Huei

AU - Lin, Wei-Chen

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Freshwater shrimps are the most common crustaceans kept in an aquarium. This study was a survey seeking parasites infecting cultured freshwater atyid shrimps at aquarium stores in Tainan, Taiwan. We observed that atyid shrimps were infested with Vorticella and Scutariella. Scutariella is a common shrimp parasite; thus, we focused on Vorticella infection in the atyid shrimps. Vorticella aequilata-like pop TW, a freshwater peritrich ciliate, was isolated from the atyid shrimps. The morphological characteristics were investigated using live observations. Specimens from the population showed identical arrangement of the infraciliature and identical ITS1-5.8SITS2 region sequences. The zooids are bell-shaped, 40–58 μm wide and 47–70 μm in long in vivo. The food vacuole is variable in shape and is located in the middle of the cell. ITS1-5.8S-ITS2 sequences of Vorticella aequilata-like pop TW did not match any available sequences in GenBank. Phylogenetically, Vorticella aequilata-like pop TW clusters with the other Vorticella within the family Vorticellidae and nests with Vorticella aequilata in the subclade. Above all, the morphological characteristics and molecular analyses show that the investigated Vorticella is a Vorticella aequilata-like species. The phylogenetic analyses of ciliates based on the ITS1-5.8S-ITS2 sequences reveal that the Vorticella genus consists of Vorticella morphospecies and that taxonomic revision of the genus is needed. Morphometric criteria and molecular analysis were used to describe and identify the Vorticella specie and this study presents the first molecular identification analysis of the Vorticella species in the cultured atyid shrimps in Tainan, Taiwan.

AB - Freshwater shrimps are the most common crustaceans kept in an aquarium. This study was a survey seeking parasites infecting cultured freshwater atyid shrimps at aquarium stores in Tainan, Taiwan. We observed that atyid shrimps were infested with Vorticella and Scutariella. Scutariella is a common shrimp parasite; thus, we focused on Vorticella infection in the atyid shrimps. Vorticella aequilata-like pop TW, a freshwater peritrich ciliate, was isolated from the atyid shrimps. The morphological characteristics were investigated using live observations. Specimens from the population showed identical arrangement of the infraciliature and identical ITS1-5.8SITS2 region sequences. The zooids are bell-shaped, 40–58 μm wide and 47–70 μm in long in vivo. The food vacuole is variable in shape and is located in the middle of the cell. ITS1-5.8S-ITS2 sequences of Vorticella aequilata-like pop TW did not match any available sequences in GenBank. Phylogenetically, Vorticella aequilata-like pop TW clusters with the other Vorticella within the family Vorticellidae and nests with Vorticella aequilata in the subclade. Above all, the morphological characteristics and molecular analyses show that the investigated Vorticella is a Vorticella aequilata-like species. The phylogenetic analyses of ciliates based on the ITS1-5.8S-ITS2 sequences reveal that the Vorticella genus consists of Vorticella morphospecies and that taxonomic revision of the genus is needed. Morphometric criteria and molecular analysis were used to describe and identify the Vorticella specie and this study presents the first molecular identification analysis of the Vorticella species in the cultured atyid shrimps in Tainan, Taiwan.

UR - http://www.scopus.com/inward/record.url?scp=85055862838&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055862838&partnerID=8YFLogxK

U2 - 10.1016/j.ijppaw.2018.10.002

DO - 10.1016/j.ijppaw.2018.10.002

M3 - Article

VL - 7

SP - 415

EP - 422

JO - International Journal for Parasitology: Parasites and Wildlife

JF - International Journal for Parasitology: Parasites and Wildlife

SN - 2213-2244

IS - 3

ER -