TY - JOUR
T1 - Five subgenotypes of hepatitis B virus genotype B with distinct geographic and virological characteristics
AU - Liu, Wen Chun
AU - Phiet, Pham Hoang
AU - Chiang, Tzen Yuh
AU - Sun, Koun Tem
AU - Hung, Kuo Hsiang
AU - Young, Kung Chia
AU - Wu, I. Chin
AU - Cheng, Pin Nan
AU - Chang, Ting Tsung
N1 - Funding Information:
This study is supported in part by the Republic of China (ROC) National Science Council (NSC 93-2314-B-006-090). We appreciate that Dr. Tomoyuki Sakamoto and Professor Masashi Mizokami, Departments of Clinical Molecular Informative Medicine, Nagoya City University Graduate School of Medical Sciences, provided the HBV/B 5 sequences.
PY - 2007/11
Y1 - 2007/11
N2 - Several hepatitis B virus (HBV) subgenotypes, HBV/A1, A2, Bj and Ba, have been reported with respect to clinical differences among patients infected with these subgenotypes. The population genetics and phylogeography of HBV were investigated based on the complete genome sequences of 484 isolates with 108 from our chronic hepatitis B patients and the remaining from the GenBank database. Besides genotypes A-H (HBV/A-H), five subgenotypes were identified among 169 HBV/B isolates by phylogenetic analysis and nucleotide divergence. There were 27 isolates of subgenotype B1 (HBV/B1) restricted to Japan, 104 isolates of HBV/B2 with the widest distribution in most Asian countries, 4 isolates of HBV/B3 restricted to Indonesia, 32 isolates of HBV/B4 restricted to Vietnam, and 7 isolates of HBV/B5 restricted to Philippines. HBV/B2-B5 isolates carried a recombination with HBV/C over the precore and core genes. In addition to the characteristics of HBV/B1-B5 at some cis-acting elements, the precore stop-codon mutant (G1896A) was significantly different among HBV/B1, HBV/B2, and HBV/B4 (70.3%, 31.7%, 53.0%, P = 0.001), while no such mutation was found in HBV/B3 and B5. Among characteristics of the HBV/B1-B5 amino acid sequences, serotype adw (K122) was exclusive among HBV/B1, HBV/B2, and HB V/B3 isolates, while serotype ayw (R122) was among the HBV/B4 and HBV/B5 isolates. Furthermore, distinct variations of T cell and B cell recognition epitopes within surface and core proteins were also found among these subgenotypes. In conclusion, subgenotypes HBV/B1-B5 exhibited distinct geographical distributions, virologic characteristics, and probable clinical implications.
AB - Several hepatitis B virus (HBV) subgenotypes, HBV/A1, A2, Bj and Ba, have been reported with respect to clinical differences among patients infected with these subgenotypes. The population genetics and phylogeography of HBV were investigated based on the complete genome sequences of 484 isolates with 108 from our chronic hepatitis B patients and the remaining from the GenBank database. Besides genotypes A-H (HBV/A-H), five subgenotypes were identified among 169 HBV/B isolates by phylogenetic analysis and nucleotide divergence. There were 27 isolates of subgenotype B1 (HBV/B1) restricted to Japan, 104 isolates of HBV/B2 with the widest distribution in most Asian countries, 4 isolates of HBV/B3 restricted to Indonesia, 32 isolates of HBV/B4 restricted to Vietnam, and 7 isolates of HBV/B5 restricted to Philippines. HBV/B2-B5 isolates carried a recombination with HBV/C over the precore and core genes. In addition to the characteristics of HBV/B1-B5 at some cis-acting elements, the precore stop-codon mutant (G1896A) was significantly different among HBV/B1, HBV/B2, and HBV/B4 (70.3%, 31.7%, 53.0%, P = 0.001), while no such mutation was found in HBV/B3 and B5. Among characteristics of the HBV/B1-B5 amino acid sequences, serotype adw (K122) was exclusive among HBV/B1, HBV/B2, and HB V/B3 isolates, while serotype ayw (R122) was among the HBV/B4 and HBV/B5 isolates. Furthermore, distinct variations of T cell and B cell recognition epitopes within surface and core proteins were also found among these subgenotypes. In conclusion, subgenotypes HBV/B1-B5 exhibited distinct geographical distributions, virologic characteristics, and probable clinical implications.
UR - http://www.scopus.com/inward/record.url?scp=35348869767&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=35348869767&partnerID=8YFLogxK
U2 - 10.1016/j.virusres.2007.07.016
DO - 10.1016/j.virusres.2007.07.016
M3 - Article
C2 - 17825452
AN - SCOPUS:35348869767
SN - 0168-1702
VL - 129
SP - 212
EP - 223
JO - Virus Research
JF - Virus Research
IS - 2
ER -
