Flow cytometric analysis of DNA in esophageal cancer

D. C. Wu, L. T. Chen, M. S. Huang, C. M. Jan, W. M. Wang, Ching-Hsun Liu, H. J. Lin, C. Y. Chen

Research output: Contribution to journalArticle

Abstract

Malignancy is the first of the ten leading causes of death in Taiwan area, however, since 1978, esophageal cancer is on the first ten causes of malignancy. Esophageal cancer is an aggressive neoplasm with a generally poor prognosis. This is usually related to the advanced stages of the neoplasm at presentation and at diagnosis. In this study, the DNA content in tumor was analyzed by flow cytometry, and compared it with clinical- pathological data, especially the survival time. All thirty seven cases (36 male, 1 female, mean age: 56.5 +/- 10.3 y/o) received operation at Kaohsiung Medical College Hospital in the past 11 years and 29 cases (27 male, 2 female, mean age: 62.2 +/- 9.6 y/o) proved to be esophageal cancer by endoscopic biopsy were assayed in this study. The formalin fixed-paraffin embedded block is used to analyze DNA content by flow cytometry. After comparing these results with clinical-pathological data, there were no significant difference in sex, age, duration, tumor location and length. However, there is a significant correlation between ploidy and tumor cell differentiation, that is, most of the diploid cells were well differentiated while most of the aneuploid cells were poorly differentiated. The median survival time of diploid cases was 325 days which was longer than the 277 days in aneuploid group, but there was no significant difference between these two groups. The same result was noticed in DNA index. Multiple variables analysis of prognostic factors disclosed that there were significant difference in correation with age tumor location tumor length, and whether there was combined radiotherapy and operation. But there were no significant relationship between survival and DNA ploidy DNA index distant metastasis surrounding tissue invasion sex duration and tumor cell differentiation. These data suggest that DNA folw cytometry analysis alone, may not provide a sueful biologic basis for the variable prognosis seen with esophageal tumors.

Original languageEnglish
Pages (from-to)542-551
Number of pages10
JournalGaoxiong yi xue ke xue za zhi = The Kaohsiung journal of medical sciences
Volume8
Issue number10
Publication statusPublished - 1992 Jan 1

Fingerprint

Esophageal Neoplasms
DNA
Neoplasms
Ploidies
Aneuploidy
Diploidy
Cell Differentiation
Flow Cytometry
Taiwan
Sex Characteristics
Paraffin
Formaldehyde
Cause of Death
Radiotherapy
Neoplasm Metastasis
Biopsy

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Wu, D. C., Chen, L. T., Huang, M. S., Jan, C. M., Wang, W. M., Liu, C-H., ... Chen, C. Y. (1992). Flow cytometric analysis of DNA in esophageal cancer. Gaoxiong yi xue ke xue za zhi = The Kaohsiung journal of medical sciences, 8(10), 542-551.
Wu, D. C. ; Chen, L. T. ; Huang, M. S. ; Jan, C. M. ; Wang, W. M. ; Liu, Ching-Hsun ; Lin, H. J. ; Chen, C. Y. / Flow cytometric analysis of DNA in esophageal cancer. In: Gaoxiong yi xue ke xue za zhi = The Kaohsiung journal of medical sciences. 1992 ; Vol. 8, No. 10. pp. 542-551.
@article{0a21f531962a4007a7b7708dc70355f4,
title = "Flow cytometric analysis of DNA in esophageal cancer",
abstract = "Malignancy is the first of the ten leading causes of death in Taiwan area, however, since 1978, esophageal cancer is on the first ten causes of malignancy. Esophageal cancer is an aggressive neoplasm with a generally poor prognosis. This is usually related to the advanced stages of the neoplasm at presentation and at diagnosis. In this study, the DNA content in tumor was analyzed by flow cytometry, and compared it with clinical- pathological data, especially the survival time. All thirty seven cases (36 male, 1 female, mean age: 56.5 +/- 10.3 y/o) received operation at Kaohsiung Medical College Hospital in the past 11 years and 29 cases (27 male, 2 female, mean age: 62.2 +/- 9.6 y/o) proved to be esophageal cancer by endoscopic biopsy were assayed in this study. The formalin fixed-paraffin embedded block is used to analyze DNA content by flow cytometry. After comparing these results with clinical-pathological data, there were no significant difference in sex, age, duration, tumor location and length. However, there is a significant correlation between ploidy and tumor cell differentiation, that is, most of the diploid cells were well differentiated while most of the aneuploid cells were poorly differentiated. The median survival time of diploid cases was 325 days which was longer than the 277 days in aneuploid group, but there was no significant difference between these two groups. The same result was noticed in DNA index. Multiple variables analysis of prognostic factors disclosed that there were significant difference in correation with age tumor location tumor length, and whether there was combined radiotherapy and operation. But there were no significant relationship between survival and DNA ploidy DNA index distant metastasis surrounding tissue invasion sex duration and tumor cell differentiation. These data suggest that DNA folw cytometry analysis alone, may not provide a sueful biologic basis for the variable prognosis seen with esophageal tumors.",
author = "Wu, {D. C.} and Chen, {L. T.} and Huang, {M. S.} and Jan, {C. M.} and Wang, {W. M.} and Ching-Hsun Liu and Lin, {H. J.} and Chen, {C. Y.}",
year = "1992",
month = "1",
day = "1",
language = "English",
volume = "8",
pages = "542--551",
journal = "Kaohsiung Journal of Medical Sciences",
issn = "1607-551X",
publisher = "Elsevier (Singapore) Pte Ltd",
number = "10",

}

Wu, DC, Chen, LT, Huang, MS, Jan, CM, Wang, WM, Liu, C-H, Lin, HJ & Chen, CY 1992, 'Flow cytometric analysis of DNA in esophageal cancer', Gaoxiong yi xue ke xue za zhi = The Kaohsiung journal of medical sciences, vol. 8, no. 10, pp. 542-551.

Flow cytometric analysis of DNA in esophageal cancer. / Wu, D. C.; Chen, L. T.; Huang, M. S.; Jan, C. M.; Wang, W. M.; Liu, Ching-Hsun; Lin, H. J.; Chen, C. Y.

In: Gaoxiong yi xue ke xue za zhi = The Kaohsiung journal of medical sciences, Vol. 8, No. 10, 01.01.1992, p. 542-551.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Flow cytometric analysis of DNA in esophageal cancer

AU - Wu, D. C.

AU - Chen, L. T.

AU - Huang, M. S.

AU - Jan, C. M.

AU - Wang, W. M.

AU - Liu, Ching-Hsun

AU - Lin, H. J.

AU - Chen, C. Y.

PY - 1992/1/1

Y1 - 1992/1/1

N2 - Malignancy is the first of the ten leading causes of death in Taiwan area, however, since 1978, esophageal cancer is on the first ten causes of malignancy. Esophageal cancer is an aggressive neoplasm with a generally poor prognosis. This is usually related to the advanced stages of the neoplasm at presentation and at diagnosis. In this study, the DNA content in tumor was analyzed by flow cytometry, and compared it with clinical- pathological data, especially the survival time. All thirty seven cases (36 male, 1 female, mean age: 56.5 +/- 10.3 y/o) received operation at Kaohsiung Medical College Hospital in the past 11 years and 29 cases (27 male, 2 female, mean age: 62.2 +/- 9.6 y/o) proved to be esophageal cancer by endoscopic biopsy were assayed in this study. The formalin fixed-paraffin embedded block is used to analyze DNA content by flow cytometry. After comparing these results with clinical-pathological data, there were no significant difference in sex, age, duration, tumor location and length. However, there is a significant correlation between ploidy and tumor cell differentiation, that is, most of the diploid cells were well differentiated while most of the aneuploid cells were poorly differentiated. The median survival time of diploid cases was 325 days which was longer than the 277 days in aneuploid group, but there was no significant difference between these two groups. The same result was noticed in DNA index. Multiple variables analysis of prognostic factors disclosed that there were significant difference in correation with age tumor location tumor length, and whether there was combined radiotherapy and operation. But there were no significant relationship between survival and DNA ploidy DNA index distant metastasis surrounding tissue invasion sex duration and tumor cell differentiation. These data suggest that DNA folw cytometry analysis alone, may not provide a sueful biologic basis for the variable prognosis seen with esophageal tumors.

AB - Malignancy is the first of the ten leading causes of death in Taiwan area, however, since 1978, esophageal cancer is on the first ten causes of malignancy. Esophageal cancer is an aggressive neoplasm with a generally poor prognosis. This is usually related to the advanced stages of the neoplasm at presentation and at diagnosis. In this study, the DNA content in tumor was analyzed by flow cytometry, and compared it with clinical- pathological data, especially the survival time. All thirty seven cases (36 male, 1 female, mean age: 56.5 +/- 10.3 y/o) received operation at Kaohsiung Medical College Hospital in the past 11 years and 29 cases (27 male, 2 female, mean age: 62.2 +/- 9.6 y/o) proved to be esophageal cancer by endoscopic biopsy were assayed in this study. The formalin fixed-paraffin embedded block is used to analyze DNA content by flow cytometry. After comparing these results with clinical-pathological data, there were no significant difference in sex, age, duration, tumor location and length. However, there is a significant correlation between ploidy and tumor cell differentiation, that is, most of the diploid cells were well differentiated while most of the aneuploid cells were poorly differentiated. The median survival time of diploid cases was 325 days which was longer than the 277 days in aneuploid group, but there was no significant difference between these two groups. The same result was noticed in DNA index. Multiple variables analysis of prognostic factors disclosed that there were significant difference in correation with age tumor location tumor length, and whether there was combined radiotherapy and operation. But there were no significant relationship between survival and DNA ploidy DNA index distant metastasis surrounding tissue invasion sex duration and tumor cell differentiation. These data suggest that DNA folw cytometry analysis alone, may not provide a sueful biologic basis for the variable prognosis seen with esophageal tumors.

UR - http://www.scopus.com/inward/record.url?scp=0026937113&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026937113&partnerID=8YFLogxK

M3 - Article

VL - 8

SP - 542

EP - 551

JO - Kaohsiung Journal of Medical Sciences

JF - Kaohsiung Journal of Medical Sciences

SN - 1607-551X

IS - 10

ER -