Abstract
The theory of antibody-dependent enhancement plays an important role in the dengue virus infection. However, its molecular mechanism is not clearly studied partially due to lack of a sensitive assay to determine the dengue virus-infected cells. We developed a flow cytometric assay with anti-dengue antibody intracellular staining on dengue virus-infected cells. Both anti-E and anti-prM Abs could enhance the dengue virus infection. The anti-prM Ab not only enhanced the dengue virus infected cell mass, but also increased the dengue virus protein synthesis within the cells. The effect of anti-prM Ab-mediated enhancement on dengue virus infection is serotype-independent. We concluded that the target cell-based flow cytometry with anti-dengue antibody intracellular staining on dengue virus-infected cells is a sensitive assay to detect the dengue virus infected cells and to evaluate the effect of enhancing antibody on dengue virus infection on cell lines or human primary monocytes.
| Original language | English |
|---|---|
| Pages (from-to) | 142-150 |
| Number of pages | 9 |
| Journal | Dengue Bulletin |
| Volume | 29 |
| Publication status | Published - 2005 Dec |
All Science Journal Classification (ASJC) codes
- Infectious Diseases
- Virology
