Flow cytometric determination for dengue virus-infected cells: Its application for antibody-dependent enhancement study

Kao Jean Huang, Yu Ching Yang, Yee Shin Lin, Hsiao Sheng Liu, Trai Ming Yeh, Shun Hua Chen, Ching Chuan Liu, Huan Yao Lei

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The theory of antibody-dependent enhancement plays an important role in the dengue virus infection. However, its molecular mechanism is not clearly studied partially due to lack of a sensitive assay to determine the dengue virus-infected cells. We developed a flow cytometric assay with anti-dengue antibody intracellular staining on dengue virus-infected cells. Both anti-E and anti-prM Abs could enhance the dengue virus infection. The anti-prM Ab not only enhanced the dengue virus infected cell mass, but also increased the dengue virus protein synthesis within the cells. The effect of anti-prM Ab-mediated enhancement on dengue virus infection is serotype-independent. We concluded that the target cell-based flow cytometry with anti-dengue antibody intracellular staining on dengue virus-infected cells is a sensitive assay to detect the dengue virus infected cells and to evaluate the effect of enhancing antibody on dengue virus infection on cell lines or human primary monocytes.

Original languageEnglish
Pages (from-to)142-150
Number of pages9
JournalDengue Bulletin
Volume29
Publication statusPublished - 2005 Dec 1

All Science Journal Classification (ASJC) codes

  • Infectious Diseases
  • Virology

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