Flow cytometric determination for dengue virus-infected cells

Its application for antibody-dependent enhancement study

Kao Jean Huang, Yu Ching Yang, Yee-Shin Lin, Hsiao-Sheng Liu, Trai-Ming Yeh, Shun-hua Chen, Ching-Chuan Liu, Huan Yao Lei

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The theory of antibody-dependent enhancement plays an important role in the dengue virus infection. However, its molecular mechanism is not clearly studied partially due to lack of a sensitive assay to determine the dengue virus-infected cells. We developed a flow cytometric assay with anti-dengue antibody intracellular staining on dengue virus-infected cells. Both anti-E and anti-prM Abs could enhance the dengue virus infection. The anti-prM Ab not only enhanced the dengue virus infected cell mass, but also increased the dengue virus protein synthesis within the cells. The effect of anti-prM Ab-mediated enhancement on dengue virus infection is serotype-independent. We concluded that the target cell-based flow cytometry with anti-dengue antibody intracellular staining on dengue virus-infected cells is a sensitive assay to detect the dengue virus infected cells and to evaluate the effect of enhancing antibody on dengue virus infection on cell lines or human primary monocytes.

Original languageEnglish
Pages (from-to)142-150
Number of pages9
JournalDengue Bulletin
Volume29
Publication statusPublished - 2005 Dec

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Antibody-Dependent Enhancement
Dengue Virus
Virus Diseases
Dengue
Anti-Idiotypic Antibodies
Staining and Labeling
Blocking Antibodies
Monocytes
Flow Cytometry

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

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abstract = "The theory of antibody-dependent enhancement plays an important role in the dengue virus infection. However, its molecular mechanism is not clearly studied partially due to lack of a sensitive assay to determine the dengue virus-infected cells. We developed a flow cytometric assay with anti-dengue antibody intracellular staining on dengue virus-infected cells. Both anti-E and anti-prM Abs could enhance the dengue virus infection. The anti-prM Ab not only enhanced the dengue virus infected cell mass, but also increased the dengue virus protein synthesis within the cells. The effect of anti-prM Ab-mediated enhancement on dengue virus infection is serotype-independent. We concluded that the target cell-based flow cytometry with anti-dengue antibody intracellular staining on dengue virus-infected cells is a sensitive assay to detect the dengue virus infected cells and to evaluate the effect of enhancing antibody on dengue virus infection on cell lines or human primary monocytes.",
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T2 - Its application for antibody-dependent enhancement study

AU - Huang, Kao Jean

AU - Yang, Yu Ching

AU - Lin, Yee-Shin

AU - Liu, Hsiao-Sheng

AU - Yeh, Trai-Ming

AU - Chen, Shun-hua

AU - Liu, Ching-Chuan

AU - Lei, Huan Yao

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