Flower color and pigmentation patterns in Phalaenopsis orchids

Chia Chi Hsu, Hong-Hwa Chen

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Orchidaceae, one of the largest plant families, is well known for its fascinating floral morphological features, specialized pollination, and distinctive ecological strategies. Phalaenopsis, with its longlasting flowers of various colors, has become important ornamental plants worldwide. However, the molecular mechanism of the anthocyanin biosynthesis is not well studied. In this chapter, the structural and regulatory genes involved in the flower color and anthocyanin biosynthetic pathway are identified and characterized in Phalaenopsis. The expression of three PeMYBs - PeMYB2, PeMYB11, and PeMYB12 - and three structural genes - PeF3H5, PeDFR1, and PeANS3 - was coincident with the anthocyanin accumulation. Transient assay of overexpression of the three PeMYBs verified that PeMYB2 resulted in anthocyanin accumulation, and these PeMYBs could activate the expression of three downstream structural genes, PeF3H5, PeDFR1, and PeANS3. In addition, these three PeMYBs participated in the distinct pigmentation patterning in a single flower, as revealed by virus-induced gene silencing. In the sepals/ petals, the silencing of PeMYB2, PeMYB11, and PeMYB12 resulted in the loss of the full-red pigmentation, red spots, and venation patterns, respectively. Moreover, different pigmentation patterning was regulated by PeMYBs in the sepals/petals and lip, PeMYB11 was responsive to the red spots in the callus of the lip, and PeMYB12 participated in the full pigmentation in the central lobe of the lip. The combined expression of these three PeMYBs in different ratios leads to a wealth of complicated floral pigmentation patterning in Phalaenopsis. This study will be beneficial for molecular breeding of new Phalaenopsis cultivars with various floral pigmentation patterns and investigation of the mechanism for many somaclonal variants with changed flower colors.

Original languageEnglish
Title of host publicationOrchid biotechnology III
PublisherWorld Scientific Publishing Co. Pte Ltd
Pages393-420
Number of pages28
ISBN (Electronic)9789813109230
ISBN (Print)9813109211, 9789813109216
DOIs
Publication statusPublished - 2017 Jan 25

Fingerprint

Orchidaceae
Anthocyanins
Phalaenopsis
Pigmentation
pigmentation
flower
Color
Genes
flowers
color
gene
anthocyanins
structural genes
lips
Lip
calyx
corolla
Biosynthesis
Viruses
pollination

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Agricultural and Biological Sciences(all)
  • Environmental Science(all)

Cite this

Hsu, C. C., & Chen, H-H. (2017). Flower color and pigmentation patterns in Phalaenopsis orchids. In Orchid biotechnology III (pp. 393-420). World Scientific Publishing Co. Pte Ltd. https://doi.org/10.1142/9789813109223_0019
Hsu, Chia Chi ; Chen, Hong-Hwa. / Flower color and pigmentation patterns in Phalaenopsis orchids. Orchid biotechnology III. World Scientific Publishing Co. Pte Ltd, 2017. pp. 393-420
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Hsu, CC & Chen, H-H 2017, Flower color and pigmentation patterns in Phalaenopsis orchids. in Orchid biotechnology III. World Scientific Publishing Co. Pte Ltd, pp. 393-420. https://doi.org/10.1142/9789813109223_0019

Flower color and pigmentation patterns in Phalaenopsis orchids. / Hsu, Chia Chi; Chen, Hong-Hwa.

Orchid biotechnology III. World Scientific Publishing Co. Pte Ltd, 2017. p. 393-420.

Research output: Chapter in Book/Report/Conference proceedingChapter

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Hsu CC, Chen H-H. Flower color and pigmentation patterns in Phalaenopsis orchids. In Orchid biotechnology III. World Scientific Publishing Co. Pte Ltd. 2017. p. 393-420 https://doi.org/10.1142/9789813109223_0019