Fluorescent immunosensing enhanced with a bead-based optoelectrokinetic platform

Han Sheng Chuang, Hsiao Neng Lin, Jen Yi Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Bead-based immunosensing is a promising technology in the point-of-care diagnostics due to its high flexibility. For dilute samples, functionalized particles can concentrate dispersed analytes and act as carriers for particle manipulation. To realize rapid and selective diagnosis, a bead-based optoelectrokinetic immunosensing technique, termed rapid electrokinetic patterning (REP), was employed herein to detect a biomarker, lipocalin 1 (LCN1), for diabetic retinopathy. The measurement was made in an aqueous droplet sandwiched between two parallel electrodes. With an electric field and a focused laser beam applying on the microchip simultaneously, the immunocomplexes in the droplet were further concentrated to enhance the fluorescent signal. The detection required only several tens of seconds. The limit of detection (LOD) reached nearly 110 pg/mL. The combined use of bead-based immunoassays and the optoelectrokinetic platform enabled a noninvasive measure to the early diagnosis of diabetic retinopathy.

Original languageEnglish
Title of host publicationProceedings of the 14th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2019
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages130-134
Number of pages5
ISBN (Electronic)9781728116297
DOIs
Publication statusPublished - 2019 Apr
Event14th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2019 - Bangkok, Thailand
Duration: 2019 Apr 112019 Apr 14

Publication series

NameProceedings of the 14th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2019

Conference

Conference14th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2019
CountryThailand
CityBangkok
Period19-04-1119-04-14

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Instrumentation

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