TY - GEN
T1 - Focal potassium microinjection in rat hippocampal slices inducing interictal activities
AU - Parittotokkaporn, Tassanai
AU - Sirirattanapan, Jarinratn
AU - Yu, Penning
AU - Hsiao, Min Chi
AU - Song, Dong
AU - Berger, Theodore W.
PY - 2011
Y1 - 2011
N2 - Focal potassium microinjection induces the incidence of epileptiform activities of rat hippocampal slices. We investigated the seizure responses of this activity in vitro using extracellular records from acute rat hippocampal slice. The electrophysiological recordings were performed on CA3 regions during microinjection of high potassium chloride. High potassium (8.5 mM) induces interictal field potentials generated spontaneously in the injected region of CA3. High-frequency bursting analogous to ictal seizure activity was locally obtained in the slice regarding to changes in cellular excitability synaptic signaling of potassium channel which may contribute to this phenomenon equivalent to the potassium bathed method. This study concludes that the focal potassium microinjection significantly initiates seizure at CA3 region and is a suitable model for investigating focal epileptogenesis in hippocampal slice.
AB - Focal potassium microinjection induces the incidence of epileptiform activities of rat hippocampal slices. We investigated the seizure responses of this activity in vitro using extracellular records from acute rat hippocampal slice. The electrophysiological recordings were performed on CA3 regions during microinjection of high potassium chloride. High potassium (8.5 mM) induces interictal field potentials generated spontaneously in the injected region of CA3. High-frequency bursting analogous to ictal seizure activity was locally obtained in the slice regarding to changes in cellular excitability synaptic signaling of potassium channel which may contribute to this phenomenon equivalent to the potassium bathed method. This study concludes that the focal potassium microinjection significantly initiates seizure at CA3 region and is a suitable model for investigating focal epileptogenesis in hippocampal slice.
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U2 - 10.1109/BMEiCon.2012.6172068
DO - 10.1109/BMEiCon.2012.6172068
M3 - Conference contribution
AN - SCOPUS:84860442705
SN - 9781457721908
T3 - BMEiCON-2011 - 4th Biomedical Engineering International Conference
SP - 269
EP - 272
BT - BMEiCON-2011 - 4th Biomedical Engineering International Conference
T2 - 4th Biomedical Engineering International Conference, BMEiCON-2011
Y2 - 29 January 2012 through 31 January 2012
ER -